Green tea proanthocyanidins inhibit cyclooxygenase-2 expression in LPS-activated mouse macrophages: Molecular mechanisms and structure–activity relationship

Hou, De‐Xing; Masuzaki, Satoko; Hashimoto, Fumio; Uto, Takuhiro; Tanigawa, Shunsuke; Fujii, Makoto; Sakata, Yusuke

doi:10.1016/j.abb.2007.01.009

Cited by 81 publications

(70 citation statements)

References 40 publications

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“…On the other hand, consistent with our findings, no activity of daidzein in the low micromolar range was observed in the study mentioned before [39]. Anti-inflammatory effects of proanthocyanidins have been reported in rats and mice [41], in human studies [42], as well as in inflammatory cell models [43][44][45]. In our study, HPE proanthocyanidins either oligomers or polymers were found to inhibit the LPS-induced release of NO, Fig.…”

Section: Discussionsupporting

confidence: 92%

Polymeric proanthocyanidins from Sicilian pistachio (Pistacia vera L.) nut extract inhibit lipopolysaccharide-induced inflammatory response in RAW 264.7 cells

et al. 2011

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Background Positive effects of pistachio nut consumption on plasma inflammatory biomarkers have been described; however, little is known about molecular events associated with these effects. Purpose We studied the anti-inflammatory activity of a hydrophilic extract from Sicilian Pistacia L. (HPE) in a macrophage model and investigated bioactive components relevant to the observed effects. Methods HPE oligomer/polymer proanthocyanidin fractions were isolated by adsorbance chromatography, and components quantified as anthocyanidins after acidic hydrolysis. Isoflavones were measured by gradient elution HPLC analysis. RAW 264.7 murine macrophages were pre-incubated with either HPE (1-to 20-mg fresh nut equivalents) or its isolated components for 1 h, then washed before stimulating with lipopolysaccharide (LPS) for 24 h. Cell viability and parameters associated with Nuclear Factor-jB (NF-jB) activation were assayed according to established methods including ELISA, Western blot, or cytofluorimetric analysis. Results HPE suppressed nitric oxide (NO) and tumor necrosis factor-a (TNF-a) production and inducible NO-synthase levels dose dependently, whereas inhibited prostaglandin E 2 (PGE 2 ) release and decreased cyclooxygenase-2 content, the lower the HPE amount the higher the effect. Cytotoxic effects were not observed. HPE also caused a dose-dependent decrease in intracellular reactive oxygen species and interfered with the NF-jB activation.Polymeric proanthocyanidins, but not isoflavones, at a concentration comparable with their content in HPE, inhibited NO, PGE 2 , and TNF-a formation, as well as activation of IjB-a. Oligomeric proanthocyanidins showed only minor effects. Conclusions Our results provide molecular evidence of anti-inflammatory activity of pistachio nut and indicate polymeric proanthocyanidins as the bioactive components. The mechanism may involve the redox-sensitive transcription factor NF-jB. Potential effects associated with pistachio nut consumption are discussed in terms of the proanthocyanidin bioavailability.

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Section: Discussionsupporting

confidence: 92%

Polymeric proanthocyanidins from Sicilian pistachio (Pistacia vera L.) nut extract inhibit lipopolysaccharide-induced inflammatory response in RAW 264.7 cells

et al. 2011

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“…9) They are widely distributed in food plants, and their biological activities depend on their chemical structures and concentration. PAs have been extracted from cacao, 22) lowbush blueberry, 22) apple, 23) grape, 24) wine, 24) green tea leaves, 25,26) and persimmon fruits. 27) PAs are known to have high structural diversity and complexity, and are distinguished by their structural characteristics, such as flavanol units, stereochemistry of C3 and C4, interflavonol linkages between C4 and C8 or C6, degree of polymerization, and acyl substituents at C3-O-.…”

Section: Discussionmentioning

confidence: 99%

Major Water-Soluble Polyphenols, Proanthocyanidins, in Leaves of Persimmon (Diospyros kaki) and Their α-Amylase Inhibitory Activity

Kawakami

Aketa

Nakanami

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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“…Compounds isomeric with catechin dimers were detected for precursor ions at m/z 577 (8-11), 593 (12)(13)(14) and 609 (15)(16). Another series, with isomeric forms, were identified as esterified catechingallate and glucuronide dimers for ions at m/z 729 (17-18), 745 (19)(20)(21)(22), 761 (23)(24)(25)(26), 785 (27)(28), 881 (29)(30)(31), 897 (32)(33), 913 (34)(35) and 937 (36)(37). The retention time of these isomeric compounds were listed in Table 1.…”

Section: Hplc/esi-it-ms Analysismentioning

confidence: 99%

“…The activities increase proportionally with the raise of the DP [23]. Proanthocyanidins is very important in human health protection and disease prevention; they have been reported to be strong antioxidants [24,25], anticancer [26], anti-ulcerogenic [27], antiprotozoal [28], cicatrizing [29], anti-inflammatory [30] agents and other beneficial properties that can suggest the application of species with tannins in pharmacologic studies, like anti-HIV and anti-HSV effects [31] and pancreatic lipase inhibition properties [32].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Proanthocyanidins from Parkia biglobosa (Jacq.) G. Don. (Fabaceae) by Flow Injection Analysis — Electrospray Ionization Ion Trap Tandem Mass Spectrometry and Liquid Chromatography/Electrospray Ionization Mass Spectrometry

et al. 2013

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Abstract:The present study investigates the chemical composition of the African plant Parkia biglobosa (Fabaceae) roots and barks by Liquid Chromatography -Electrospray Ionization and Direct Injection Tandem Mass Spectrometry analysis. Mass spectral data indicated that B-type oligomers are present, namely procyanidins and prodelphinidins, with their gallate and glucuronide derivatives, some of them in different isomeric forms. The analysis evidenced the presence of up to 40 proanthocyanidins, some of which are reported for the first time. In this study, the antiradical activity of extracts of roots and barks from Parkia biglobosa was evaluated using DPPH method and they showed satisfactory activities.

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Green tea proanthocyanidins inhibit cyclooxygenase-2 expression in LPS-activated mouse macrophages: Molecular mechanisms and structure–activity relationship

Cited by 81 publications

References 40 publications

Polymeric proanthocyanidins from Sicilian pistachio (Pistacia vera L.) nut extract inhibit lipopolysaccharide-induced inflammatory response in RAW 264.7 cells

Polymeric proanthocyanidins from Sicilian pistachio (Pistacia vera L.) nut extract inhibit lipopolysaccharide-induced inflammatory response in RAW 264.7 cells

Major Water-Soluble Polyphenols, Proanthocyanidins, in Leaves of Persimmon (Diospyros kaki) and Their α-Amylase Inhibitory Activity

Characterization of Proanthocyanidins from Parkia biglobosa (Jacq.) G. Don. (Fabaceae) by Flow Injection Analysis — Electrospray Ionization Ion Trap Tandem Mass Spectrometry and Liquid Chromatography/Electrospray Ionization Mass Spectrometry

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