Green Tea Polyphenols Alleviate Obesity in Broiler Chickens through the Regulation of Lipid-Metabolism-Related Genes and Transcription Factor Expression

Huang, Jinbao; Zhang, Yong; Zhou, Yibin; Zhang, Zhengzhu; Xie, Zhongwen; Zhang, Jinsong; Wan, Xiaochun

doi:10.1021/jf402004x

Cited by 98 publications

(97 citation statements)

References 42 publications

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“…PPAR-α, as a member of nuclear receptors, is highly expressed in the liver and plays an important role in regulating glucose and lipid metabolism or enhancing the related-gene expression in fatty acid oxidation (Lee et al, 1995;Zhang et al, 2011;Huang et al, 2013). LPL, on the other hand, catalyzes triglycerides from circulating chylomicron and very low-density lipoprotein to generate fatty acids and glycerol for tissue utilization or storage (Zhao et al, 2013).…”

Section: Effect Of Cottonseed Meal Fermented With Yeast On the Lipid-mentioning

confidence: 99%

“…Nutritionists have tried to mitigate this problem using nutritional strategies, such as the dietary supplementation of plant extracts and probiotics, and some researchers have demonstrated that this is an efficient means of preventing excessive fat deposition. Huang et al (2013) reported that the dietary supplementation of green tea polyphenols (extracted from tea plants) reduced abdominal and subcutaneous fat deposition in broiler chickens by regulating the expression of key genes related to lipid metabolism. Furthermore, Aluwong et al (2013) showed that the supplementation of a yeast probiotic in broiler feeds significantly decreased abdominal fat weight by 16.67% in the group fed 1.5% and by 28.626% in the group fed 2.0% compared with the controls.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Cottonseed Meal Fermented with Yeast on the Lipid-related Gene Expression in Broiler Chickens

Nie

et al. 2015

Rev. Bras. Cienc. Avic.

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Fermented cottonseed meal (FCSM) is widely used in poultry diets in China. This study was conducted to investigate the effect of FCSM on lipid-related gene expression in broilers. Initially, 180 broiler chickens (21-days-old, equal number of males and females) were randomly divided into three groups, with six pens per group and 10 birds per pen. The chickens in the control group were fed a diet containing unfermented cottonseed meal, and those in the treatment groups were fed with diets including either CSM fermented by Candida tropicalis (Ct group) or CSM fermented by Candida tropicalis plus Saccharomyces cerevisae (Ct-Sc group) until 64 days old. The results revealed that, compared with the control group (p<0.05), the mRNA expression of peroxisome proliferator-activated receptor alpha (PPAR-α) and lipoprotein lipase (LPL) were upregulated in the livers of Ct-Sc males. The expression of PPAR-α was also upregulated in the livers of Ct females. The expression levels of acetyl CoA carboxylase (ACC) and LPL in the liver of males and the expression of PPAR-α in the liver of females were significantly different between the Ct and Ct-Sc groups (p<0.05). However, gene expressions of fatty acid synthase (FAS) and liver fatty acid-binding protein (L-FABP) in the liver were not altered when the broilers were fed FCSM-supplemented diets (p>0.05). Likewise, the expressions of peroxisome proliferator-activated receptor gamma (PPAR-γ) and LPL in the abdominal fat were not altered by the FCSMsupplemented diets (p>0.05). The results in this study indicate that CSM fermented by Candida tropicalis and Saccharomyces cerevisiae effectively regulated the genes involved in fatty acid β-oxidation and triglyceride hydrolysis in male broiler chickens. Furthermore, the effects of the FCSM-supplemented diets were significantly different between bird sexes and between yeast strains used in the fermentation process.

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Section: Effect Of Cottonseed Meal Fermented With Yeast On the Lipid-mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Cottonseed Meal Fermented with Yeast on the Lipid-related Gene Expression in Broiler Chickens

Nie

et al. 2015

Rev. Bras. Cienc. Avic.

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“…Real-time qPCR was used to verify six LM proteins of differential abundance at the mRNA level by the method of Huang et al (2013) with slight modifications.…”

Section: Validation Of Proteins Of Differential Abundancementioning

confidence: 99%

“…The primer sequences are shown in Table S1. The relative fold-change was calculated by the 2 −ΔΔC T method (Huang et al, 2013).…”

Section: Validation Of Proteins Of Differential Abundancementioning

confidence: 99%

iTRAQ-based quantitative proteomic analysis of longissimus muscle from growing pigs with dietary supplementation of non-starch polysaccharide enzymes

Zhang

Gao

et al. 2015

J. Zhejiang Univ. Sci. B

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Non-starch polysaccharide enzymes (NSPEs) have long been used in the feed production of monogastric animals to degrade non-starch polysaccharide to oligosaccharides and promote growth performance. However, few studies have been conducted on the effect of such enzymes on skeletal muscle in monogastric animals. To elucidate the mechanism of the effect of NSPEs on skeletal muscle, an isobaric tag for relative and absolute quantification (iTRAQ) for differential proteomic quantitation was applied to investigate alterations in the proteome in the longissimus muscle (LM) of growing pigs after a 50-d period of supplementation with 0.6% NSPEs in the diet. A total of 51 proteins were found to be differentially expressed in the LM between a control group and the NSPE group. Functional analysis of the differentially expressed protein species showed an increased abundance of proteins related to energy production, protein synthesis, muscular differentiation, immunity, oxidation resistance and detoxification, and a decreased abundance of proteins related to inflammation in the LM of the pigs fed NSPEs. These findings have important implications for understanding the mechanisms whereby dietary supplementation with NSPEs enzymes can promote growth performance and improve muscular metabolism in growing pigs.

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“…In particular, green tea polyphenols (GTPs) have been shown to inhibit adipogenesis and induce apoptosis in mouse 3T3-L1 preadipocytes [17,18] . Similarly, GTPs have been shown to reduce obesity and serum lipid levels in broiler chickens by suppressing fatty acid synthesis and stimulating lipolysis [19] . This study was conducted to examine the effects of different green tea extracts (GTEs) on body and organ weight and expression of key adipogenic transcripts in broiler chickens.…”

Section: Introductionmentioning

confidence: 99%

Potent Anti-Adipogenic Effect of Green Tea and Green Tea Extracts in Chicken

Kim¹,

Regassa²,

#³

et al. 2017

JDO

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This study was conducted to examine the effect of green tea on body, adipose tissue, and liver weights and adipose tissue weight to body weight ratio and the adipogenic differentiation and expression of adipogenic transcripts in chicken (Gallus gallus) preadipocytes. In experiment one, chicks were weighed and randomly assigned to a control diet (CTRL) and green tea treatment (GT 1%, w/w) for 38 days. In experiment 2, preadipocytes were isolated from 20 wk old chicken and treated with an adipogenic cocktail (DMIOA) containing 500 nM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine, 20 µg/mL insulin, and 300 μM OA, DMIOA+30 µg of extract B, E, H, Mc, T, and W, respectively, for 48 h. Data were analysed using the General Liner Model procedure of the Statistics Analysis System (SAS) Institute version 9.4, and differences were considered significant at P < 0.05. Gene expression was measured using quantitative real-time PCR. GT 1% significantly reduced body (-9%; P = 0.0447), liver (-20%; P = 0.0206), and abdominal fat weight (-44%; P = 0.0055) compared with the control (CTRL) group (GT 0%). The abdominal fat/body weight ratio of green tea supplemented group (-36%; P = 0.0125) was also significantly lower than that of control group. In cell culture study, all green tea extracts inhibited C/EBPα and β mRNA expression compared to DMIOA. DMIOA+B, T, or W reduced mRNA expression of FABP4 by three-fold compared to DMIOA. Although all green tea extracts reduced adipocyte formation, T and W had the strongest anti-adipogenic effects. These results demonstrate that supplementation of green tea could be an effective strategy in the control of obesity in chickens.

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Green Tea Polyphenols Alleviate Obesity in Broiler Chickens through the Regulation of Lipid-Metabolism-Related Genes and Transcription Factor Expression

Cited by 98 publications

References 42 publications

Effect of Cottonseed Meal Fermented with Yeast on the Lipid-related Gene Expression in Broiler Chickens

Effect of Cottonseed Meal Fermented with Yeast on the Lipid-related Gene Expression in Broiler Chickens

iTRAQ-based quantitative proteomic analysis of longissimus muscle from growing pigs with dietary supplementation of non-starch polysaccharide enzymes

Potent Anti-Adipogenic Effect of Green Tea and Green Tea Extracts in Chicken

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