Green tea polyphenol extract attenuates lung injury in experimental model of carrageenan-induced pleurisy in mice

Abstract

Here we investigate the effects of the green tea extract in an animal model of acute inflammation, carrageenan-induced pleurisy. We report here that green tea extract (given at 25 mg/kg i.p. bolus 1 h prior to carrageenan), exerts potent anti-inflammatory effects in an animal model of acute inflammation in vivo.Injection of carrageenan (2%) into the pleural cavity of mice elicited an acute inflammatory response characterized by fluid accumulation in the pleural cavity that contained many neutrophils (PMNs), an infiltration of PMNs in lung tissues and increased production of nitrite/nitrate, tumour necrosis factor alpha. All parameters of inflammation were attenuated by green tea extract treatment. Furthermore, carrageenan induced an up-regulation of the adhesion molecule ICAM-1, as well as nitrotyrosine and poly (ADP-ribose) synthetase (PARS) formation, as determined by immunohistochemical analysis of lung tissues. Staining for the ICAM-1, nitrotyrosine, and PARS was reduced by green tea extract.Our results clearly demonstrate that treatment with green tea extract exerts a protective effect and offers a novel therapeutic approach for the management of lung injury.

Inhibition property of green tea extract in relation to reserpine-induced ribosomal strips of rough endoplasmic reticulum (rER) of the rat kidney proximal tubule cells

“…Our observation suggests that green tea extract, when administered with reserpine, may result in kidney remedial, which, therefore, appears to be responsible for reports exist in literature, supporting the role of green tea in oxidative stress-damage recovery in animal models (Das et al, 2002;Baltaziak et al, 2004;Chen et al, 2004;Di Paola et al, 2005;Hisamura, et al, 2006;Itoh et al, 2005;Yokozawa et al, 2005;Upaganlawar et al, 2006). Green tea extract represents a rich source of natural polyphenols that has in vivo protective effects on liver and serum.…”

Proinflammatory Role of Glucocorticoid-Induced TNF Receptor-Related Gene in Acute Lung Inflammation

“…P-selectin (CD62P; BD Pharmingen), myeloperoxidase, ICAM-1 (CD54; BD Pharmingen), and nitrotyrosine Abs (Upstate Biotechnology) were used, as previously described (33). The polyclonal anti-GITR (R&D Systems) and anti-ZO-1 Abs were used 1/100 in the same experimental conditions as the others.…”

“…Tissue sections were prepared, as previously described (33). P-selectin (CD62P; BD Pharmingen), myeloperoxidase, ICAM-1 (CD54; BD Pharmingen), and nitrotyrosine Abs (Upstate Biotechnology) were used, as previously described (33).…”

“…For TNF-␣ and IL-1␤, ELISA tests were conducted, as previously described (33). PGE 2 was measured by RIA without prior extraction or purification (34).…”

Identification, quantitation, and method validation for flavan‐3‐ols in fermented ready‐to‐drink teas from the Italian market using HPLC‐UV/DAD and LC‐MS/MS

“…A consistent amount is active and shows an interesting bioavailability ranging from 4.5% for gallic acid to 8.7% for (À)-epigallocatechingallate (EGCG) and EGC. The biological activities of tea polyphenols include antioxidative capability on LDL, antiatherosclerotic, hypocholesterolaemic, antihypertensive, antiallergic, antimicrobial, and anticarcinogenic properties [7][8][9][10][11]. Several studies [12,13] demonstrated that tea catechins can scavenge reactive oxygen species (ROS), thanks to the phenolic hydroxyl group present in the flavan-3-ol structure, and also prevent their formation by inhibiting the xanthine oxidase [14].…”