Green Tea Inhibits Uterine Contractility in Ex Vivo (Non-Pregnant) Mice Models

Abstract

Green tea is widely known for its beneficial biologic effect and there have been some reports on the beneficial effect on the reproductive system. There have however been no reports on green tea effects on uterine contractility. This study is therefore aimed at the investigation of green tea extract on the amplitude and frequency of uterine contractility. Green tea bags were macerated in boiling water for 5 min and concentrated to dryness. The extract (0.33 -1333.21 µg/mL) was tested on the isolated mouse uterus. The contractility parameters investigated included spontaneous contraction, oxytocin (60 pg/mL) induced contraction and high KCl (80 mM)-induced contraction. High resolution mass spectrometric (HRMS) determination of secondary metabolites was also performed on the extract. The extract inhibited both the amplitude and frequency of uterine contractility studied, however minimal inhibitory effect was observed with KCl-induced contraction. The HRMS analysis revealed the presence of twenty-five (25) significant compounds, 23 of which were identified and 2 were unknown. Compounds were observed to belong to a diverse range of phytochemical classes including pteridine, flavonoids, cyclitols, and coumarins, with the majority of detected compounds belonging to the flavonoid class. The results obtained in this study have shown that green tea extract inhibits uterine contractility possibly due to the presence of the flavonoids, and through interaction with calcium and/or prostaglandins.

Elucidating Flavonoid and Antioxidant Activity in Edible and Medicinal Herbs Woodwardia japonica (L.f.) Sm. Based on HPLC-ESI-TOF-MS and Artificial Neural Network Model: Response to Climatic Factors

“…With UV λmax at 283 nm, molecular ions at m / z [M-H] − 463.0882, [M+H] + 465.1026 and [2M-H] − 927.1837, C 21 H 20 O 12 was automatically matched to by the software, and peak 6 was tentatively identified as isotrifolin [ 22 ]. Peak 7 with UV λmax at 265, 285 and 350 nm, and molecular ions at m / z [M-H] − 609.1461, [M+H] + 611.1621.…”

An Immunoinformatic of Epigallocatechin-3-O-gallate as Adjuvant Therapy of Periodontitis: An in-silico Study

“…Furthermore, EGCG can develop mineralized bone nodules. [8][9][10] Thus, this study aims to investigate the immunoinformatic of EGCG as an adjuvant periodontitis therapy in silico.…”