Green Tea Extract Treatment in Obese Mice with Nonalcoholic Steatohepatitis Restores the Hepatic Metabolome in Association with Limiting Endotoxemia‐TLR4‐NFκB‐Mediated Inflammation

Sasaki, Geoffrey Y.; Li, Jinhui; Cichon, Morgan J.; Riedl, Ken M.; Kopec, Rachel; Bruno, Richard S.

doi:10.1002/mnfr.201900811

Cited by 31 publications

(23 citation statements)

References 56 publications

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“…To take further insight into the HBV-speci c serum lipodomics, we integrated the differential lipids [26]. On the other hand, PCs take an essential place in the membrane integrity [27,28].…”

Section: Discussionmentioning

confidence: 99%

Chronic HBV Infection Associates with Improvement of Lipid Profile and Steatosis-Related Impairment in Nonalcoholic Fatty Liver Disease: A Lipidomic Analysis

Han

Xie

et al. 2020

Preprint

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BackgroundChronic hepatitis B virus (HBV) infection exerts an impact on lipid metabolism, but its interaction with dysmetabolism-based nonalcoholic fatty liver disease (NAFLD) remains uncertain. The present study performs lipidomic and pathological comparison in NAFLD patients with or without chronic HBV infection so as to highlight its effects on lipid metabolism and metabolic liver disease.MethodsBiopsy-proven Chinese NAFLD patients with (NAFLD-HBV group, n=21) or without chronic HBV infection (NAFLD group, n=41) were enrolled in the case-control study. Their serum lipid profile was subjected to individual investigation by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS). Steatosis, activity, and fibrosis (SAF) scoring revealed the NAFLD-specific pathological characteristics. The effects of chronic HBV infection on demographic and clinical manifestations, serum lipidomics, and pathological characteristics were statistically assessed.ResultsChronic HBV infection associated with global alteration of serum lipid profile in NAFLD patients. Upregulation of phosphatidylcholine (PCs), choline plasmalogen (PC-Os) and downregulation of free fatty acids (FFAs), lysophosphatidylcholine (LPCs) dominated the HBV-related lipidomic characteristics. Compared to those of NAFLD group, the levels of serum hepatoxic lipids (FFAs: FFA16:0, FFA16: 1, FFA18:1, FFA18:2) were significantly lowered in the NAFLD-HBV group. These low-level FFAs demonstrated correlation to statistical improvements in aspartate aminotransferase activity (FFA16:0, r = 0.34, P = 0.003; FFA16:1, r = 0.37, P < 0.001; FFA18:1, r = 0.33, P < 0.001; FFA18:2, r = 0.42, P < 0.001), hepatocyte steatosis (FFA16: 1, r = 0.39, P = 0.01; FFA18:1, r = 0.39, P = 0.015; FFA18:2, r = 0.33, P = 0.036), and ballooning (FFA16:0, r = 0.31, P = 0.037; FFA16:1, r = 0.45, P < 0.001; FFA18:1, r = 0.37, P = 0.005; FFA18:2, r=0.30, P = 0.013).ConclusionChronic HBV infection may present improvements in the serum lipidomics and steatosis-related pathological characteristics of NAFLD.Trial registrationTrial registration number: ChiCTR-DDT-13003983. Registered 13 May, 2013, http://www.chictr.org.cn/enIndex.aspx?proj=5584.

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Section: Discussionmentioning

confidence: 99%

Chronic HBV Infection Associates with Improvement of Lipid Profile and Steatosis-Related Impairment in Nonalcoholic Fatty Liver Disease: A Lipidomic Analysis

Han

Xie

et al. 2020

Preprint

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“…Interestingly, the plasma level of the flavan-3-ols metabolite, 5-(3′-hydroxyphenyl)-γ-valerolactone-4′-sulfate, was significantly positively correlated to flow-mediated vasodilatation. Noteworthy, mice on high-fat diet improved non-alcoholic fatty liver disease by lessening IR and endotoxin-toll-like receptor 4/NF- κ B pathway in response to raised hepatic catechin metabolites (namely phenyl- γ -valerolactones) following catechin-rich green tea extract [ 289 ].…”

Section: Polyphenol Metabolitesmentioning

confidence: 99%

Insight into Polyphenol and Gut Microbiota Crosstalk: Are Their Metabolites the Key to Understand Protective Effects against Metabolic Disorders?

et al. 2020

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Lifestyle factors, especially diet and nutrition, are currently regarded as essential avenues to decrease modern-day cardiometabolic disorders (CMD), including obesity, metabolic syndrome, type 2 diabetes, and atherosclerosis. Many groups around the world attribute these trends, at least partially, to bioactive plant polyphenols given their anti-oxidant and anti-inflammatory actions. In fact, polyphenols can prevent or reverse the progression of disease processes through many distinct mechanisms. In particular, the crosstalk between polyphenols and gut microbiota, recently unveiled thanks to DNA-based tools and next generation sequencing, unravelled the central regulatory role of dietary polyphenols and their intestinal micro-ecology metabolites on the host energy metabolism and related illnesses. The objectives of this review are to: (1) provide an understanding of classification, structure, and bioavailability of dietary polyphenols; (2) underline their metabolism by gut microbiota; (3) highlight their prebiotic effects on microflora; (4) discuss the multifaceted roles of their metabolites in CMD while shedding light on the mechanisms of action; and (5) underscore their ability to initiate host epigenetic regulation. In sum, the review clearly documents whether dietary polyphenols and micro-ecology favorably interact to promote multiple physiological functions on human organism.

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“…In a typical green tea extract (GTE), EGCG is believed to be the major factor responsible for the biological effects [ 2 , 3 , 4 , 5 ]. Among the many biological effects attributed to green tea, catechins were found to be associated with potential anticancer activity [ 6 , 7 , 8 ], to interact with gut microbiota [ 9 , 10 ], and to protect against nonalcoholic steatohepatitis [ 11 , 12 ]. More interestingly, for the purpose of the present work, green tea catechins improved cardiovascular and metabolic health [ 13 , 14 , 15 , 16 ], one aspect of which was the provision of protection against diet-induced obesity [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Standardized Green Tea Extract and Its Main Component, EGCG, on Mitochondrial Function and Contractile Performance of Healthy Rat Cardiomyocytes

et al. 2020

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We recently showed that the long-term in vivo administration of green tea catechin extract (GTE) resulted in hyperdynamic cardiomyocyte contractility. The present study investigates the mechanisms underlying GTE action in comparison to its major component, epigallocatechin-3-gallate (EGCG), given at the equivalent amount that would be in the entirety of GTE. Twenty-six male Wistar rats were given 40 mL/day of a tap water solution with either standardized GTE or pure EGCG for 4 weeks. Cardiomyocytes were then isolated for the study. Cellular bioenergetics was found to be significantly improved in both GTE- and EGCG-fed rats compared to that in controls as shown by measuring the maximal mitochondrial respiration rate and the cellular ATP level. Notably, the improvement of mitochondrial function was associated with increased levels of oxidative phosphorylation complexes, whereas the cellular mitochondrial mass was unchanged. However, only the GTE supplement improved cardiomyocyte mechanics and intracellular calcium dynamics, by lowering the expression of total phospholamban (PLB), which led to an increase of both the phosphorylated-PLB/PLB and the sarco-endoplasmic reticulum calcium ATPase/PLB ratios. Our findings suggest that GTE might be a valuable adjuvant tool for counteracting the occurrence and/or the progression of cardiomyopathies in which mitochondrial dysfunction and alteration of intracellular calcium dynamics constitute early pathogenic factors.

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Green Tea Extract Treatment in Obese Mice with Nonalcoholic Steatohepatitis Restores the Hepatic Metabolome in Association with Limiting Endotoxemia‐TLR4‐NFκB‐Mediated Inflammation

Abstract: Green tea extract treatment in obese mice with nonalcoholic steatohepatitis restores the hepatic metabolome in association with limiting endotoxemia-TLR4-NFκB-mediated inflammation

Cited by 31 publications

References 56 publications

Chronic HBV Infection Associates with Improvement of Lipid Profile and Steatosis-Related Impairment in Nonalcoholic Fatty Liver Disease: A Lipidomic Analysis

Chronic HBV Infection Associates with Improvement of Lipid Profile and Steatosis-Related Impairment in Nonalcoholic Fatty Liver Disease: A Lipidomic Analysis

Insight into Polyphenol and Gut Microbiota Crosstalk: Are Their Metabolites the Key to Understand Protective Effects against Metabolic Disorders?

Effects of Standardized Green Tea Extract and Its Main Component, EGCG, on Mitochondrial Function and Contractile Performance of Healthy Rat Cardiomyocytes

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