Green Tea Extract in the Extender Improved the Post-Thawed Semen Quality and Decreased Amino Acid Mutation of Kacang Buck Sperm

Susilowati, Suherni; Mustofa, Imam; Wurlina, Wurlina; Hernawati, Tatik; Oktanella, Yudit; Soeharsono, Soeharsono; Purwanto, Djoko

doi:10.3390/vetsci9080403

Cited by 7 publications

(15 citation statements)

References 59 publications

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“…Egg yolk (5 mL; derived from laboratory chicken eggs) was added to 95 mL of skim milk solution, then added with 1 IU/mL of penicillin (Meiji Seika Pharma, Tokyo, Japan) and 1 μg/mL of streptomycin (Thermo Fisher Scientific, Singapore). 7 The solution was divided into five equal volumes without addition of EGCG CNPs for the control group (T0) and with addition of 0.5, 1.0, 1.5, and 2.0 μg of EGCG CNPs/ mL extender for T1, T2, T3 and T4 groups, respectively.…”

Section: Sm-ey Extendermentioning

confidence: 99%

“…The filled and sealed straws were chilled in liquid nitrogen vapor from 5°C to À 140°C for 10 min, and immediately stored in liquid nitrogen (À196°C) for 24 hours before evaluation were conducted. 7…”

Section: Frozen Semenmentioning

confidence: 99%

“…Six replicates randomly were used to assess sperm IPM, viability, progressive motility, MDA levels, catalase levels, and DPPH scavenging, respectively, according to methods reported in a previous study. 7…”

Section: Evaluation Of Post-thawed Sperm Qualitymentioning

confidence: 99%

“…Sperm with an IPM showed a curved tail, whereas those with a damaged plasma membrane showed a straight tail. 7 Viability A drop of semen sample and a drop of nigrosine (Sigma-Aldrich) were mixed and smeared on a glass slide, after which the slide was dried over a flame. The slide was then examined under a light microscope (Olympus BX-53, Tokyo, Japan) at 400Â magnification to evaluate the percentage of live sperm in 100 spermatozoa.…”

Section: Ipmmentioning

confidence: 99%

“…5 Therefore, antioxidants are needed to counteract the effects of ROS and thereby increase post-thawed semen quality. 6 In our previous studies, green tea extract was used to improve semen quality and decrease nucleotide mutations in mtDNA 3 and protein-encoded mtDNA, 7 presumably due to an increase in the antioxidant capacity.…”

Section: Introductionmentioning

confidence: 99%

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Epigallocatechin-3-gallate chitosan nanoparticles in an extender improve the antioxidant capacity and post-thawed quality of Kacang goat semen

Mustofa

Susilowati²,

Suprayogi³

et al. 2023

F1000Res

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Background and Aim: The Kacang goat (Capra hircus) is an indigenous livestock species in Indonesia that is at risk of extinction due to cross-breeding. Artificial insemination (AI) techniques are expected to increase the population of these goats. This study aimed to determine the addition of epigallocatechin-3-gallate chitosan nanoparticles (EGCG CNPs) to skim milk–egg yolk (SM–EY) extender to obtain the best possible quality of post-thawed Kacang buck semen for AI. Materials and Methods: Fresh Kacang buck semen was diluted in SM–EY without or with the addition of 0.5, 1.0, 1.5, or 2.0 µg of EGCG CNPs/mL extender. Extended semen was packaged in French mini straws, frooze, and stored in liquid nitrogen at −196℃ for 24 hours. Six replicates from each treatment group were thawed for catalase, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, malondialdehyde (MDA), sperm intact plasma membrane (IPM), viability and motility analyses. Results: Post-thawed semen that was previously frozen without EGCG CNPs in the extender (control group) exhibited the lowest levels of catalase, DPPH, sperm viability, sperm motility, IPM, and the highest levels of MDA. However, the addition of EGCG CNPs at doses of 1.5 µg/mL extender increased post-thawed catalase, DPPH, sperm IPM, viability, and sperm motility and decreased MDA levels (p < 0.05) than those of control group. Conclusion: This study was the first in which EGCG CNPs were used in SM–EY extender, and the addition of only 1.0 µg/mL of EGCG CNPs in this extender increased the antioxidant capacity and post-thawed quality of Kacang buck semen.

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Section: Sm-ey Extendermentioning

confidence: 99%

Section: Frozen Semenmentioning

confidence: 99%

Section: Evaluation Of Post-thawed Sperm Qualitymentioning

confidence: 99%

Section: Ipmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Epigallocatechin-3-gallate chitosan nanoparticles in an extender improve the antioxidant capacity and post-thawed quality of Kacang goat semen

Mustofa

Susilowati²,

Suprayogi³

et al. 2023

F1000Res

Self Cite

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Calliandra honey and nutritional deficiency influence to spermatozoa motility and viability in Rattus norvegiccus

Adikara,

Wurlina,

Mulyati

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Nutritional deficiency or malnutrition is one of the severe health problems that causes changes in body weight, body composition, and physical function. The potential of calliandra honey to spermatozoa motility and viability, in the male white rat (Rattus norvegiccus) undergoing nutritional deficiency will be investigated in this research. 24 male white rats with body weights 250-300 grams were used and divided into four groups with each group containing six white rats. The first group was the negative control (C-) group, C+ was the group with fasting condition but without honey administration, T1 and T2 were the fasting treatment in ten days with 30% and 50% honey, respectively. The result was significance differences between C-group with C+ or T1 and T2 (p<0.05). The T1 and T2 can increase the motility and viability of spermatozoa and the difference significant with C+ (p<0.05), but it cannot restore the motility and viability of spermatozoa to normal conditions. It can be concluded that the administration of Calliandra honey in the T1 and T2 with concentrations of 30% and 50% can already improve the quality of spermatozoa which can be seen from the motility and viability of spermatozoa.

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Investigation of the effects of syringic acid supplemented to Tris semen diluent on ram semen freezability

Avdatek

̇İnanç

Gülhan

et al. 2023

Reprod Domestic Animals

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To the best of our knowledge, no research has been conducted to test the effects of syringic acid (SA) on ram semen freezing within the scope of natural antioxidants added to semen extenders. Therefore, this study had two main objectives. First, to test whether adding SA to ram semen freezing extender has a protective effect and contributes positively to sperm kinetic, plasma and acrosome integrity, mitochondrial membrane potential, lipid peroxidation, oxidant and antioxidant and DNA damage parameters after thawing. Second, it was to determine at what concentration the SA supplemented to the extender could be applied by in vitro studies by preserving the fertilization ability of frozen semen at the highest level. In the study, six individuals of Sönmez rams were used. The semen was collected from the rams using an artificial vagina and pooled. The pooled semen was divided into five different groups and extended with 0, 0.5, 1, 2 and 4 mM SA (control C, SA0.5, SA1, SA2 and SA4, respectively). After dilution, the semen samples were kept at 4°C for 3 h, then loaded into 0.25 mL straws and frozen in liquid nitrogen vapour. The SA1 and SA2 groups were higher plasma membrane and acrosome integrity (PMAI), high mitochondrial membrane potential (HMMP), plasma membrane integrity and motility compared to other groups (p < .05). It was observed that SA supplemented to the Tris extender significantly reduced DNA damage, and the lowest values were obtained especially in the SA1 and SA2 treatments (p < .05). Also, lowest MDA level was determined at the SA1 and this was statistically significant compared to SA4 and C (p < .05). In conclusion, it was revealed that SA added to Tris semen extender at 1 and 2 mM treatment doses increased progressive and total motility and preserved PMAI, plasma membrane integrity, HMMP and DNA integrity.

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Green Tea Extract in the Extender Improved the Post-Thawed Semen Quality and Decreased Amino Acid Mutation of Kacang Buck Sperm

Cited by 7 publications

References 59 publications

Epigallocatechin-3-gallate chitosan nanoparticles in an extender improve the antioxidant capacity and post-thawed quality of Kacang goat semen

Epigallocatechin-3-gallate chitosan nanoparticles in an extender improve the antioxidant capacity and post-thawed quality of Kacang goat semen

Calliandra honey and nutritional deficiency influence to spermatozoa motility and viability in Rattus norvegiccus

Investigation of the effects of syringic acid supplemented to Tris semen diluent on ram semen freezability

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