Green tea component epigallocatechin-3-gallate decreases expression of osteopontin via a decrease in mRNA half-life in cell lines of metastatic hepatocellular carcinoma

Zapf, Matthew; Kothari, Anai N.; Weber, Cynthia E.; Arffa, Matthew; Wai, Philip Y.; Driver, Joseph; Gupta, Gopal N.; Kuo, Paul C.; Mi, Zhiyong

doi:10.1016/j.surg.2015.06.011

Cited by 17 publications

(12 citation statements)

References 28 publications

(35 reference statements)

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“…Our cell migration assay demonstrated that miR-221 is necessary for EGCG-induced delay in wound closure, and miR-221 can mimic the effects of EGCG. Previous work from our lab has demonstrated that EGCG treatment at these concentrations does not affect cell viability [25], and therefore should have minimal consequences on wound closure. Alamar Blue bioassay also demonstrated that miR-221 modulation does not affect cell viability when concomitantly treated with TAA with or without EGCG (S1 Fig).…”

Section: Discussionmentioning

confidence: 99%

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Epigallocatechin-3-Gallate Upregulates miR-221 to Inhibit Osteopontin-Dependent Hepatic Fibrosis

et al. 2016

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Osteopontin (OPN) promotes hepatic fibrosis, and developing therapies targeting OPN expression in settings of hepatic injury holds promise. The polyphenol epigallocatechin-3-gallate (EGCG), found in high concentrations in green tea, downregulates OPN expression through OPN mRNA degradation, but the mechanism is unknown. Previous work has shown that microRNAs can decrease OPN mRNA levels, and other studies have shown that EGCG modulates the expression of multiple microRNAs. In our study, we first demonstrated that OPN induces hepatic stellate cells to transform into an activated state. We then identified three microRNAs which target OPN mRNA: miR-181a, miR-10b, and miR-221. In vitro results show that EGCG upregulates all three microRNAs, and all three microRNAs are capable of down regulating OPN mRNA when administered alone. Interestingly, only miR-221 is necessary for EGCG-mediated OPN mRNA degradation and miR-221 inhibition reduces the effects of EGCG on cell function. In vivo experiments show that thioacetamide (TAA)-induced cell cytotoxicity upregulates OPN expression; treatment with EGCG blocks the effects of TAA. Furthermore, chronic treatment of EGCG in vivo upregulates all three microRNAs equally, suggesting that in more chronic treatment all three microRNAs are involved in modulating OPN expression. We conclude that in in vitro and in vivo models of TAA-induced hepatic fibrosis, EGCG inhibits OPN-dependent injury and fibrosis. EGCG works primarily by upregulating miR-221 to accelerate OPN degradation. EGCG may therefore have utility as a protective agent in settings of liver injury.

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Section: Discussionmentioning

confidence: 99%

“…EGCG-mediated OPN mRNA degradation leads to decreased wound closure [25]. However, the mechanism and the effects of EGCG on OPN in vivo have not been evaluated.…”

Section: Introductionmentioning

confidence: 99%

Epigallocatechin-3-Gallate Upregulates miR-221 to Inhibit Osteopontin-Dependent Hepatic Fibrosis

et al. 2016

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“…For these reasons, EGCG is able to diminish tumor angiogenesis and stall growth [ 72 ]. In addition, there is strong evidence that EGCG is capable of diminishing migration and metastasis formation of tumors [ 73 , 74 ]. Previous studies report that EGCG promotes a reduction in the migration and metastasis formation of tumor cells with tumor size reduction, accomplishing a more reliable and efficient chemotherapy [ 75 – 77 ].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Potential of Epigallocatechin Gallate Nanodelivery Systems

Granja

Frias

Neves

et al. 2017

BioMed Research International

128

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Nowadays, the society is facing a large health problem with the rising of new diseases, including cancer, heart diseases, diabetes, neurodegenerative diseases, and obesity. Thus, it is important to invest in substances that enhance the health of the population. In this context, epigallocatechin gallate (EGCG) is a flavonoid found in many plants, especially in tea. Several studies support the notion that EGCG has several benefits in fighting cancer, heart diseases, diabetes, and obesity, among others. Nevertheless, the poor intestinal absorbance and instability of EGCG constitute the main drawback to use this molecule in prevention and therapy. The encapsulation of EGCG in nanocarriers leads to its enhanced stability and higher therapeutic effects. A comprehensive review of studies currently available on the encapsulation of EGCG by means of nanocarriers will be addressed.

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“…Also, the catechins EGCG downregulates OPN expression by reducing the half-life of EGCG mRNA, EGCG-mediated mRNA degradation leads to reduced closure of wounds (Zapf et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Gene Set Enrichment Analysis (GSEA) of Differentially Expressed Genes in Green Tea against Methionine-Choline Deficient Diet in High-Fat Patients in the Development of Non-Alcoholic Fatty Liver Disease (NAFLD)

Saha¹,

Ahammad²,

Barmon³

2018

Preprint

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The most common liver disorder nowadays is non-alcoholic fatty liver disease(NAFLD) and it is a progressive disease that rises in severity from steatosis to nonalcoholic steatohepatitis(NASH), fibrosis and cirrhosis to increase risk of developing hepatocellular carcinoma. It is a cause of great concern as there is an estimated seventy million Americans who are currently affected by NAFLD, and this is expected to only increase because of its association with obesity and diabetes and also a lack of therapies to keep its development and progression in check. In this particular study we performed a gene set enrichment analysis(GSEA) of differentially expressed genes in a green tea against methionine-choline deficient diet in high-fat patients in the development of non-alcoholic fatty liver disease(NAFLD). The downregulated genes were used to perform an enrichment analysis and in the ARCHS4 TFs Coexpression database the most significant gene was found to be KLF5_human_tf_ARCHS4_coexpression. In the ARCHS4 Kinases Coexpression pathway database STYK1_human_kinase_ARCHS4 Coexpression was found to be the most significant gene. And finally for the upregulated genes a similar enrichment analysis was performed and in the humancy database γ-linolenate biosynthesis_Homo sapiens_PWY-6000 gene was discovered to be the most significant one. This study has used bioinformatics tools and the Enrichr software to perform a comparative analysis of differentially expressed gene sets for high-fat patients having a diet consisting of green tea against a methionine-choline deficient diet. Green tea is known to contain several antioxidants and polyphenols which provide protection against many liver diseases such as non-alcoholic fatty liver disease(NAFLD). The present study simply tries to build awareness of this to the general public and allow them to learn more about certain diets which have protective effects against liver diseases. Hopefully by implementing these in their daily lifestyles the public can gain some form of protection against these types of liver disorders.

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Green tea component epigallocatechin-3-gallate decreases expression of osteopontin via a decrease in mRNA half-life in cell lines of metastatic hepatocellular carcinoma

Cited by 17 publications

References 28 publications

Epigallocatechin-3-Gallate Upregulates miR-221 to Inhibit Osteopontin-Dependent Hepatic Fibrosis

Epigallocatechin-3-Gallate Upregulates miR-221 to Inhibit Osteopontin-Dependent Hepatic Fibrosis

Therapeutic Potential of Epigallocatechin Gallate Nanodelivery Systems

Gene Set Enrichment Analysis (GSEA) of Differentially Expressed Genes in Green Tea against Methionine-Choline Deficient Diet in High-Fat Patients in the Development of Non-Alcoholic Fatty Liver Disease (NAFLD)

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