Green tea and glycine aid in the recovery of tendinitis of the Achilles tendon of rats

Vieira, Cristiano Pedrozo; Guerra, Flávia Da Ré; Oliveira, Letícia Prado; Almeida, Marcos dos Santos de; Marcondes, Maria Cristina Cintra Gomes; Pimentell, E R

doi:10.3109/03008207.2014.983270

Cited by 12 publications

(24 citation statements)

References 55 publications

(68 reference statements)

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“…The effects of glycine in vivo model of induced‐tendinitis include de novo synthesis of collagen, non‐collagenous proteins, glycosaminoglycans (GAGs), and the activation of MMP‐2 and inhibition of MMP‐9. Moreover, biomechanical properties are improved after the treatment of animals with glycine (Vieira et al, , ). Nevertheless, the molecular mechanism of glycine effects in tenocytes in vitro under inflammatory conditions it not yet known.…”

Section: Introductionmentioning

confidence: 99%

Glycine improves the remodeling process of tenocytes in vitro

Vieira

Viola

Carneiro

et al. 2018

Cell Biology International

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Tendinitis changes the biochemical and morphological properties of the tendon, promoting an increase of activity of metalloproteinases and disorganization of collagen bundles. Tenocytes, the primary cells in tendon, are scattered throughout the collagenic fibers, and are responsible of tendon remodeling and tissue repair in pathological condition. In vivo, glycine, component of the typical Gly-X-Y collagen tripeptide, showed beneficial effects in biochemical and biomechanical properties of Achilles tendon with tendinitis. In this study, we analyzed the effect of glycine in tenocytes subjected to inflammation. Tenocytes from Achilles tendon of rats were treated with TNF-α (10 ng/mL) with and without previous treatment with glycine (20 mM). Cell proliferation and migration were evaluated, as well as the expression of matrix molecules such as glycosaminoglycans, metalloproteinases (MMPs), TIMPs, and collagen I. Glycine can revert the inflammation due to the action of TNF-α by controlling the MMPs quantity and activity. These data indicated that the molecules involved to remodeling process of extracellular matrix are modulated both by TNF-α and the availability of collagen precursors; in fact, this study indicates the glycine can be useful for treatment of inflammation and for modulating tenocytes metabolism in tendons.

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Section: Introductionmentioning

confidence: 99%

Glycine improves the remodeling process of tenocytes in vitro

Vieira

Viola

Carneiro

et al. 2018

Cell Biology International

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“…It was apparent that the components involved in the remodeling process of the extracellular matrix in the MTJ of the Achilles tendon, mainly in the compaction of the collagen fibers and MMPs activities, are involved during tendon recovery. After induction of tendinitis and treatment with green tea and glycine, the extracellular matrix components of the MTJ exhibit different profiles when compared with results already published for the entire Achilles tendon (Vieira et al, ). The present study demonstrates some potentially important differences that are region‐specific in the tendon.…”

Section: Resultsmentioning

confidence: 75%

“…To induce tendinitis, the animals were first anesthetized with isoflurane (Forame) and given an intratendineous injection of 10 μL of collagenase (10 mg/mL; Sigma, C6885) dissolved in sterile buffered saline (Lake et al, ; Vieira et al, ) in the osteotendinous region of Achilles tendon. After the induction of tendinitis, the animals of the treated groups received green tea orally and a glycine diet during 7 days.…”

Section: Methodsmentioning

confidence: 99%

“…Tendinitis is characterized by the pronounced disorganization of the collagen fibers through the degradation of the collagen molecules, and alterations in biomechanical and biochemical processes, mainly in the activity of the enzymes found in the extracellular matrix (Vieira et al, ). Tendinitis is the first step in a remodeling process, and must be controlled to avoid a more severe inflammation associated with excessive degradation, such as tendinosis.…”

Section: Introductionmentioning

confidence: 99%

“…Numerous researchers are seeking practical and viable alternatives for the treatment of tendon pathologies. The inclusion of glycine and green tea in the diet has been tested as a treatment of collagenase‐induced inflammations of the Achilles tendon, with beneficial results being found in biomechanical features and the activity of MMP such as MMP‐2 and MMP‐9 (Vieira et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Green Tea and Glycine Modulate the Activity of Metalloproteinases and Collagen in the Tendinitis of the Myotendinous Junction of the Achilles Tendon

Vieira

Oliveira

Guerra

et al. 2016

The Anatomical Record

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The myotendinous junction (MTJ) is the weakest element in the muscle-tendon unit of the heel, and thus the most susceptible to injuries. The scarcity of adequate treatments means that tendinitis is a major concern to athletes and other groups who depend on their physical fitness, although green tea and glycine have both been shown to have beneficial effects on the inflammation. The present study investigated the remodeling effects of green tea and glycine in the MTJ of rats with tendinitis. For this, male Wistar rats were divided into five groups: animals without tendinitis and animals with tendinitis; animals with tendinitis supplied with green tea; animals with tendinitis supplied with a glycine diet; animals with tendinitis supplied with a green tea and glycine diet. Tendinitis was induced and the treatment with green tea (700 mg/kg/day) and a 5% glycine diet lasted 7 days. The treatments regulated the activity of metalloproteinases (MMP)-2, -8, and -9, and induced the synthesis of type I collagen, glycosaminoglycans, and noncollagenous proteins. Changes were also noted in the compaction of the collagen molecules and the amount of tenocytes. When combined, green tea and glycine modulated the inflammatory process and induced the synthesis of the elements involved in the post-lesion recovery of the tissue. The data from the MTJ were different when compared with results already published using the whole Achilles tendon. These data indicate that each region of the inflamed tendon can exhibit different responses

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A Natural Hydrogel with Prohealing Properties Enhances Tendon Regeneration

Dang

Chen

Sefat

et al. 2022

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Tendon regeneration and reduction of peritendinous adhesion remain major clinical challenges. This study addresses these challenges by adopting a unique hydrogel derived from the skin secretion of Andrias davidianus (SSAD) and taking advantage of its biological effects, adhesiveness, and controllable microstructures. The SSAD‐derived hydrogel contains many cytokines, which could promote tendon healing. In vitro, leach liquid of SSAD powder could promote tendon stem/progenitor cells migration. In vivo, the SSAD‐derived hydrogel featuring double layers possesses strong adhesiveness and could reconnect ruptured Achilles tendons of Sprague‐Dawley rats without suturing. The intimal SSAD‐derived hydrogel, with a pore size of 241.7 ± 21.0 µm, forms the first layer of the hydrogel to promote tendon healing, and the outer layer SSAD‐derived hydrogel, with a pore size of 3.3 ± 1.4 µm, reducing peritendinous adhesion by serving as a dense barrier. Additionally, the SSAD‐derived hydrogel exhibits antioxidant and antibacterial characteristics, which further contribute to the reduction of peritendinous adhesion. In vivo studies suggest that the SSAD‐derived hydrogel reduces peritendinous adhesion, increases collagen fiber deposition, promotes cell proliferation, and improves the biomechanical properties of the regenerated tendons, indicating better functional restoration. The SSAD‐derived bilayer hydrogel may be a feasible biomaterial for tendon repair in the future.

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Green tea and glycine aid in the recovery of tendinitis of the Achilles tendon of rats

Cited by 12 publications

References 55 publications

Glycine improves the remodeling process of tenocytes in vitro

Glycine improves the remodeling process of tenocytes in vitro

Green Tea and Glycine Modulate the Activity of Metalloproteinases and Collagen in the Tendinitis of the Myotendinous Junction of the Achilles Tendon

A Natural Hydrogel with Prohealing Properties Enhances Tendon Regeneration

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