Green Synthesized ZnO Nanoparticles Mediated by Streptomyces plicatus: Characterizations, Antimicrobial and Nematicidal Activities and Cytogenetic Effects

Abstract

Zinc oxide nanoparticles (ZnO-NPs) are regarded as one of the most promising kinds of materials in a variety of fields, including agriculture. Therefore, this study aimed to biosynthesize and characterize ZnO-NPs and evaluate their different biological activities. Seven isolates of actinomycetes were obtained and screened for ZnO-NPs synthesis. The isolate MK-104 was chosen and identified as the Streptomyces plicatus MK-104 strain. The biosynthesized ZnO-NPs exhibited an absorbance peak at 350 nm and were spherical in shape with an average size of 21.72 ± 4.27 nm under TEM. XRD and DLS methods confirmed these results. The biosynthesized ZnO-NPs demonstrated activity against plant pathogenic microbes such as Erwinia amylovora, Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, Fusarium moniliform and Alternaria alternata, with MIC values ranging from 15.6 to 500 µg/mL. Furthermore, ZnO-NPs had a significant effect on Meloidogyne incognita, with death percentages of 88.2, 93.4 and 96.72% after 24, 48 and 72 h of exposure, respectively. Vicia faba seeds were treated with five concentrations of ZnO-NPs (12.5, 25, 50, 100 and 200 µg/mL). Low-moderate ZnO-NP concentrations (12.5–50 µg/mL) were shown to promote seed germination and seedling development, while the mitotic index (MI) decreased as the dosage of ZnO-NPs increased. Micronuclei (MNs) and the chromosomal abnormality index increased as well.

WITHDRAWN: Cytotoxicity and wound healing efficacy of biosynthesis ZnO Nanoparticles Using Aspergillus niger against Multidrug Resistant Pseudomonas aeruginosa

“…are present in spectra with wavenumbers below 500 cm − 1 . The ZnO stretching mode is responsible for the 400-900 cm − 1 range of the peaks, demonstrating the formation and purity of the ZnO structure [32]. Our nding was in accordance with Chen et al [33] and Ashwini et al [34] who noticed that ZnO is present, as seen by the stretching at 485 cm − 1 .…”

New Gd(I)/Cs(III) complexes of benzil‐based thiocarbohydrazone macrocyclic ligand: Chemical synthesis, characterization, and study their biological effectiveness as antibacterial, antioxidant, and antiviral additives for polyurethane surface coating

“…The antibacterial activity of paint (P), gadolinium (G), paint + gadolinium (P + G), cesium (C), and paint + cesium (P + C) against bacterial pathogens was screened utilizing the agar well diffusion assay, [ 45 ] and the results were listed in Table 5 and depicted in Figure 7. The results indicated that paint alone showed no activity against the tested bacterial pathogens while gadolinium chelate affected all tested bacteria with an inhibition zone ranged from 12 to 16 mm.…”

“…The plates were left for 2 h at 4°C followed by incubation at 37°C for 18–24 h. After incubation, the inhibition zones were measured and recorded in duplicates. [ 45 ]…”

Black ZnO nanoparticles synthesized by a green chemistry process

“…However, for both processes, the requirement of complex equipment, hazardous substances, critical temperature and pressure process, time-consuming and energy-intensive procedures together with generation of environmental pollution through the production of by-products; has triggered the search for a more environmentally safe methodology [8,20]. In this sense, green synthesis has become a beneficial alternative to the production of nanomaterials using supplies such as plants, seeds, roots, peels extracts, fungi, algae or bacteria [22][23][24][25][26][27][28][29][30][31][32][33][34] as reduction reagents and stabilization of transition metal salts into metal and metal oxide nanoparticles. ZnO NPs have been successfully attained within the green chemistry approach with potential applications for industrial wastewater treatment [31,32,[35][36][37][38] biological control of plant diseases and plant growth promoter [29,39], anti-cancer drug or as antiangiogenic agent [27,28,33]; and also demonstrating a good biocompatibility, antibacterial, antimicrobial, antifungal activity [12,13,29,30].…”

“…In this sense, green synthesis has become a beneficial alternative to the production of nanomaterials using supplies such as plants, seeds, roots, peels extracts, fungi, algae or bacteria [22][23][24][25][26][27][28][29][30][31][32][33][34] as reduction reagents and stabilization of transition metal salts into metal and metal oxide nanoparticles. ZnO NPs have been successfully attained within the green chemistry approach with potential applications for industrial wastewater treatment [31,32,[35][36][37][38] biological control of plant diseases and plant growth promoter [29,39], anti-cancer drug or as antiangiogenic agent [27,28,33]; and also demonstrating a good biocompatibility, antibacterial, antimicrobial, antifungal activity [12,13,29,30]. The photocatalytic performance of ZnO NPs for degradation of malachite green (MG), methylene blue (MB), rhodamine B dyes and dibenzothiophene contaminant (DBT) has been positively tested [14,18,31,32,35].…”