Green‐synthesized Zinc oxide nanoparticle, an efficient safe anticancer compound for human breast MCF7 cancer cells

Abstract

There are many types of researches investigating anticancer therapeutics for breast cancer therapy. Zinc oxide nanoparticles (ZnONPs) as an efficient drug delivery system, has been widely being used in various biomedical applications. In the current study, we synthesized ZnONP applying Rheum rhaponticum Waste (RRW) as a novel bio‐platform to investigate its anticancer impacts on MCF7 breast cancer cells compared with normal Human HFF and HDF cells. In this regard, RRW was triggered to synthesize the ZnONPs. Then, they were characterized by XRD, FTIR, TEM, and SEM analysis. Next, the MCF‐7, HFF, and HDF cell lines were cultured and treated as the following plane: Incubation of all cell lines for 72, 48, and 24 hours at the presence of different ZnONPs doses. Finally, the cell morphology, BCL2‐ BAX genes expression profile and AO/PI‐fluorescent cell staining on the 48‐hour incubated cells were analyzed to check the ZnONP apoptotic activity. Moreover, the ZnONP antioxidant activity was analyzed by a DPPH antioxidant test. We produced the 30 nm ZnONPs which significantly increased the BAX and decreased the BCL‐2 gene expression. According to the results including the Sub G1 enhancement peaks, apoptotic hallmarks, MTT assay, and the AO/PI‐fluorescent stained cells, ZnONPs can specifically induce apoptotic death in MCF7 breast cancer cells compared with normal HFF and HDF cells. The IC50 values of MCF‐7 in 72, 48, and 24 hr were measured at 8, 11, and 12 μg/ml in 72, 48, and 24 hr, respectively. This is while the mentioned values in the normal cells (HFF, HDF) were estimated at higher treatment doses. In conclusion, we suggest that the ZnONPs have the potential to be applied as a safe cell‐specific apoptosis inducer in breast cancer treatment. However, there are many challenges that need to be clarified for applying them as an efficient anticancer agent.

3D imaging of ZnO NP distribution and ROS accumulation in MCF-7 cells and quantification of retention dynamics using laser scanning confocal microscopy

“…Fluorescent nanoformulations have been an attractive research topic in the field of cancer research both for imaging and therapeutic purposes. Specifically, ZnO NPs are one of the potential anticancer agents against MCF-7 cells and can be used for treatment of breast cancer (Sadhukhan et al, 2019;Hong et al, 2011;Ma et al, 2015;Sureshkumar et al, 2017;Gupta et al, 2015;Kavithaa et al, 2016;Salari et al, 2020;Boroumand Moghaddam et al, 2017;Farasat et al, 2020;Lestari et al, 2018;Wahab et al, 2014). However, quantitative imaging of spatial distribution of NPs in living cells over days was not investigated much.…”

ZnO nanoparticles stimulate oxidative stress to induce apoptosis of B16F10 melanoma cells: In vitro and in vivo studies

“…The live and dead cells were measured by the Acridine Orange (AO) and Propidium Iodide (PI) [27]. B16F10 and 3T3L1 cells (5×10 4 cells/well) were seeded in 12-well plates and incubated for 12 h. After exposing to different concentrations (20, 30 and 35 μg ml −1 ) of ZnO NPs for 24 h, the cells were washed and stained with AO/PI (UE, China) for 5 min in the dark.…”