Green synthesized selenium nanoparticles for ovarian cancer cell apoptosis

Amiri, Hamed; Hashemy, Seyed Isaac; Sabouri, Zahra; Javid, Hossein; Darroudi, Majid

doi:10.1007/s11164-021-04424-8

Cited by 22 publications

(6 citation statements)

References 59 publications

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“…Critically, in all of the above-mentioned papers, selenium nanoparticles were prepared by employing the chemical synthetic route. It is noteworthy that biogenic selenium nanoparticles, specifically, have been demonstrated to destroy ovarian cancer cells by triggering apoptosis [ 76 ].…”

Section: Discussionmentioning

confidence: 99%

Anticancer Activity of Biogenic Selenium Nanoparticles: Apoptotic and Immunogenic Cell Death Markers in Colon Cancer Cells

Spyridopoulou

Aindelis

Pappa

et al. 2021

Cancers

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Colorectal cancer is a health problem with high mortality rates and prevalence. Thus, innovative treatment approaches need to be developed. Biogenic nanoparticles are nanomaterials that can be synthesised in biological systems and, compared to chemically synthesised nanoparticles, have better bioavailability while being more cost-effective, eco-friendlier, and less toxic. In our previous studies, the probiotic strain Lactobacillus casei ATCC 393 was used to synthesise selenium nanoparticles (SeNps), which were shown to inhibit colon cancer cell growth in vitro and in vivo. Herein, we have further investigated SeNps’ pro-apoptotic activity and their ability to induce immunogenic cell death (ICD) in colon cancer cells. The SeNps’ effect on Caco-2 cells growth was examined along with their potential to induce caspase activation. Moreover, the expression of typical pro-apoptotic and ICD markers were examined in SeNps-treated HT29 and CT26 cells by flow cytometry, Western blot, ELISA and fluorescence microscopy. Elevated caspase-3 activation and surface phosphatyldoserine, that subsided upon co-incubation with a pan-caspase inhibitor, were detected in SeNps-treated cells. Furthermore, nanoparticles induced modulation of the expression of various apoptosis-related proteins. We also report the detection of biomarkers involved in ICD, namely the translocation of calreticulin and ERp57, the release of HMGB1 and ATP, and the secretion of pro-inflammatory cytokines from SeNps-treated cells. Moreover, RAW246.7 macrophages exhibited a higher rate of phagocytosis against treated CT26 when compared to control cells. Taken together, our findings indicate that treatment with SeNps might be an efficient strategy to destroy tumour cells by inducing apoptotic cell death and triggering immune responses.

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Section: Discussionmentioning

confidence: 99%

Anticancer Activity of Biogenic Selenium Nanoparticles: Apoptotic and Immunogenic Cell Death Markers in Colon Cancer Cells

Spyridopoulou

Aindelis

Pappa

et al. 2021

Cancers

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“…Therefore, many anti-tumor agents have been developed to induce the apoptotic process in cancer cells by targeting apoptosis-related genes [73,74]. Meanwhile, several studies have suggested that metallic NPs can show anti-cancer activity against various cancer cells via increasing the expression of proapoptotic factors and reducing the expression of anti-apoptotic factors.…”

Section: Real-time Pcrmentioning

confidence: 99%

Green synthesis of cerium oxide nanoparticles using Falcaria Vulgaris leaf extract and its anti-tumoral effects in prostate cancer

Taherzadeh

Amiri

Ebrahimi

et al. 2023

Preprint

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Over recent years, metal oxide nanoparticles have been extensively investigated due to their potential biomedical applications. This study aimed to describe a simple and eco-friendly method for the green synthesis of cerium oxide nanoparticles (CeO2 NPs) using Falcaria vulgaris (F. vulgaris) leaf extract as a capping agent and to evaluate their anti-cancer effects on human prostate cancer cells (PC3). The phyto-synthesized CeO2 NPs were characterized by UV–visible spectroscopy, X-ray diffraction, transmission electron microscope, Fourier transform infrared spectroscopy, scanning electron microscope, and energy-dispersive X-ray spectroscopy. The UV-Visible spectrum showed a peak at 313 nm with a band gap energy of 3 eV. Microscopic results revealed that CeO2 NPs mainly were spherical. In addition, the XRD pattern confirmed the crystalline structure of green synthesized CeO2 NPs with an average crystalline size of around 19.5 nm. In biological experiments, the results of the resazurin assay indicated the significant cytotoxic activity of CeO2 NPs against PC3 cells with an IC50 at 113.6 μg/ mL. The real-time PCR results revealed that CeO2 NPs effectively regulate PC3 cell proliferation, apoptosis, and migration by modifying the expression of cell cycle-related proteins (c-Myc, cyclin D1, p21), and apoptosis-related genes (Bcl-2 and Bax), and migration-related genes (MMP-2 and MMP-9). Overall, these findings suggest that the green fabricated CeO2 NPs from F. Vulgaris can be used as a novel therapeutic agent for prostate cancer treatment.

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“…Combining different metals to enhance the efficacy as well as the characteristics of the combined drug. Thorough our literature study, Se-NPs have shown a novel potential to cause the intracellular ROS reaction against ovarian cancer cells (A2780) that induces apoptosis due to the upregulation of P53 and Bax genes ( Amiri et al, 2021 ). The same also induces DNA damage in HepG2 cells, resulting in cell cycle arrest, which inhibited the proliferation of cancer cells ( Li et al, 2018 Fouda et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Padina boergesenii mediated synthesis of Se-ZnO bimetallic nanoparticles for effective anticancer activity

Thirupathi,

Pongen,

Kaveriyappan

et al. 2024

Front. Microbiol.

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IntroductionEvaluating the anticancer property of Padina boergesenii mediated bimetallic nanoparticles.MethodsThe present study focuses on synthesizing Se-ZnO bimetallic nanoparticles from an aqueous algal extract of brown algae Padina boergesenii.Synthesized Se-ZnO NPs were characterized by UV, FTIR, SEM-EDS and HRTEM for confirmation along with the anticancer activity by MTT assay.ResultsThe UV gave an absorbance peak at 342 and 370 nm, and the FTIR showed functional groups involved in synthesizing Se-ZnO NPs. The TEM micrographs indicated the crystalline nature and confirmed the size of the Se-ZnO NPs to be at an average size of 26.14 nm. Anticancer efficacy against the MCF-7 breast and HepG2 (hepatoblastoma) cell lines were also demonstrated, attaining an IC50 value of 67.9 µg and 74.9 µg/ml respectively, which caused 50% cell death.DiscussionThis work aims to highlight an effective method for delivering bioactive compounds extracted from brown algae and emphasize its future therapeutic prospects. The potential of Selenium-Zinc oxide nanoparticles is of great interest due to the biocompatibility and low toxicity aspects of selenium combined with the cost-effectiveness and sustainability of zinc metal. The presence of bioactive compounds contributed to the stability of the nanoparticles and acted as capping properties.

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Green synthesized selenium nanoparticles for ovarian cancer cell apoptosis

Cited by 22 publications

References 59 publications

Anticancer Activity of Biogenic Selenium Nanoparticles: Apoptotic and Immunogenic Cell Death Markers in Colon Cancer Cells

Anticancer Activity of Biogenic Selenium Nanoparticles: Apoptotic and Immunogenic Cell Death Markers in Colon Cancer Cells

Green synthesis of cerium oxide nanoparticles using Falcaria Vulgaris leaf extract and its anti-tumoral effects in prostate cancer

Padina boergesenii mediated synthesis of Se-ZnO bimetallic nanoparticles for effective anticancer activity

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