Green synthesized AgNPs from Periploca hydaspidis Falc. and its biological activities

Abstract

Characterization of bio‐synthesized silver nanoparticles (AgNPs) using Periploca hydaspidis (PHAgNPs) whole plant extract for the first time via UV–Visible spectroscopy, XRD, FTIR, DLS, and SEM analysis techniques was done. A rich variety of phytochemicals in P. hydaspidis aqueous extract (PHA) functioned as possible reducing and capping agents for AgNPs synthesis. In vitro antioxidant activities (DPPH, Iron chelating, Hydroxyl ion, Nitric oxide, and β‐carotene bleaching assays) of PHAgNPs revealed least IC50 values especially in hydroxyl ion (39.08 ± 0.88 μg/mL) and nitric oxide (37.53 ± 2.24 μg/mL) scavenging assays relative to standard controls (ascorbic acid, rutin, and gallic acid) and PHA. In addition, visible inhibition zone diameters were formed around discs against all pathogenic microbial strains including multi‐drug resistant strains (MDR's). MIC and MBC/MFC were depicted least in PHAgNPs with maximum bactericidal/fungicidal effects. MTT assay displayed a significant antiproliferative potential of PHAgNPs against HCCLM3, MCF‐7, MDA‐MB 231, and HEPG2 cancer cell lines, where least IC50 values were recorded against HEPG2 (12.97 ± 0.04 μg/mL) and MCF‐7 (5.73 ± 0.22 μg/mL). Furthermore, PHAgNPs considerably (p > 0.001) prevented the migration of MCF‐7 cancer cells in vitro whereas in in vivo wound healing assay, faster skin regeneration, and epithelization in wound biopsies was observed via histological analysis. PHAgNPs treated group rats significantly increased (p < 0.05) the wound contraction rate, hydroxyproline content and hemostatic potential compared to control and PHA‐treated groups.

Use of Metallic Nanoparticles Synthesized from Plant Extracts in the Wound Healing – a Review

“…Measurements performed at 0, 24, and 48 hours of incubation AgNPs were reported as a stimulant for collagen production and deposition in the wound site. Wound closure rates of 23.32% ± 2.29 and 17.21% ± 1.00 were observed at a concentration of 25 μg/mL after 24 h and 48 h of incubation, respectively [80] Cotyledon orbiculata A cut was performed on the animal's back and treated with the plant extract or the AgNPs Plant extract (20%, 35%, and 75%) and AgNPs (30%, 60%, and 100%), presented a higher wound closure rate when compared to the negative control (25%, 45%, and 85%) for specific experimental days, in this case 5, 10, and 15 days, respectively [82] 4. Conclusions Several biomedical applications of metallic NPs have been extensively studied.…”

Silver Nanoparticles Inhibit Metastasis of 4T1 Tumor in Mice after Intragastric but Not Intravenous Administration

“…Several studies have also tested a potential anticancer activity of different AgNPs formulations. A majority of these studies are limited to in vitro models and, in most cases, report that AgNPs are cytotoxic to many cancer cell lines and inhibit their migration in vitro [ 4 , 5 , 6 , 7 , 8 ]. There are fewer studies analyzing AgNPs anticancer activity in animal models, but also reveal inhibition of tumor growth in vivo [ 9 , 10 , 11 , 12 , 13 ].…”