Green Synthesized Iron Oxide Nanoparticles Effect on Fermentative Hydrogen Production by Clostridium acetobutylicum

Abstract

A green synthesis of iron oxide nanoparticles (FeNPs) was developed using Murraya koenigii leaf extract as reducing and stabilizing agent. UV-vis spectra show that the absorption band centred at a wavelength of 277 nm which corresponds to the surface plasmon resonances of synthesized FeNPs. Fourier transform infrared spectroscopy spectrum exhibits that the characteristic band at 580 cm(-1) is assigned to Fe-O of γ-Fe2O3. Transmission electron microscopy image confirms that the spherical with irregular shaped aggregates and average size of nanoparticles was found to be ∼59 nm. The effect of synthesized FeNPs on fermentative hydrogen production was evaluated from glucose by Clostridium acetobutylicum NCIM 2337. The hydrogen yield in control experiment was obtained as 1.74 ± 0.08 mol H2/mol glucose whereas the highest hydrogen yield in FeNPs supplemented experiment was achieved as 2.33 ± 0.09 mol H2/mol glucose at 175 mg/L of FeNPs. In addition, the hydrogen content and hydrogen production rate were also increased from 34 ± 0.8 to 52 ± 0.8 % and 23 to 25.3 mL/h, respectively. The effect of FeNPs was compared with supplementation of FeSO4 on fermentative process. The supplementation of FeNPs enhanced the hydrogen production in comparison with control and FeSO4. The supplementation of FeNPs led to the change of the metabolic pathway towards high hydrogen production due to the enhancement of ferredoxin activity. The fermentation type was shifted from butyrate to acetate/butyrate fermentation type at the addition of FeNPs.

Magnetically separable γ-Fe2O3 nanoparticles: An efficient catalyst for acylation of alcohols, phenols, and amines using sonication energy under solvent free condition

“…Iron oxide is an important inorganic material having magnetic properties as well as good stability in aqueous dispersion [2]. Particularly, Fe 2 O 3 nanoparticles find wide applications in gas sensing [3], Li-ion batteries [4,5], magnetizations and water treatment [6,7], hydrogen production [8], cancer treatment [9], surface enhanced Raman scattering and electrocatalysis [10], degradation of pharmaceutical compounds [11], supercapacitor [12] and as a heterogeneous catalyst [13][14][15].…”

Effects of phytogenic copper nanoparticles on fermentative hydrogen production by Enterobacter cloacae and Clostridium acetobutylicum

“…Phytofabrication of Cu-NPs using M. koenigii leaf extract M. koenigii leaf extract was prepared, as described earlier [15]. Briefly, 4.0 g of shade dried and finely powdered leaves were boiled for 5 min with 100 mL of distilled water and allowed to cool in room temperature.…”

“…The headspace biogas was analyzed using a gas chromatograph (GC-2014 Shimadzu) with a thermal conductivity detector (TCD). In addition, the volatile fatty acids (acetate, butyrate and propionate) and ethanol from liquid phase were estimated by the gas chromatograph with flame ionization detector (FID) and stabilwax-DA capillary column, as mentioned earlier [15].…”

“…Many metal and metal oxide NPs (Au, Ag, Fe, CeO 2 , ZnO, CuO, SiO 2 and TiO 2 ) found to inhibit the microorganisms [10]. However, some metal NPs such as Au [11], hematite [12], Ag [13] and iron [14,15] enhanced the fermentative hydrogen production process. Earlier, the addition of Cu 2þ in an anaerobic digestion inhibited the formation of volatile fatty acids including acetate, butyrate and propionate [16].…”

Biosynthesis of Iron Nanoparticles Using Tie Guanyin Tea Extract for Degradation of Bromothymol Blue

“…Since the inception of the new century, biosynthesis of nanoparticles as an emerging highlight of nanotechnology has received increasing attention due to a growing need to develop environmentally benign technologies in materials synthesis [21][22][23][24]. For example, a great deal of effort has been put into green synthesis of metal nanoparticles, using microorganisms, chitosan, cellulose nanocrystal, and plants [25][26][27][28][29][30][31][32].…”