Green synthesis of ZnO nanoparticles using whey as an effective chelating agent

Soares, Victor Alves; Xavier, Mary; Rodrigues, Eriverton da Silva; Oliveira, Carlos Alberto de; Farias, Patrícia M. A.; Stingl, A.; Ferreira, Nilson S.; Silva, Marcelo Souza da

doi:10.1016/j.matlet.2019.126853

Cited by 44 publications

(19 citation statements)

References 17 publications

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“…The peaks showed the characteristic hexagonal wurtzite structure of ZnO (JCPDS card no. 36-1451) [42]. The Wurtzite structure was prevalent because it is stable in ambient conditions.…”

Section: Discussionmentioning

confidence: 99%

Trichoderma harzianum-Mediated ZnO Nanoparticles: A Green Tool for Controlling Soil-Borne Pathogens in Cotton

Zaki

Ouf

Albarakaty

et al. 2021

JoF

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ZnO-based nanomaterials have high antifungal effects, such as inhibition of growth and reproduction of some pathogenic fungi, such as Fusarium sp., Rhizoctonia solani and Macrophomina phaseolina. Therefore, we report the extracellular synthesis of ZnONPs using a potential fungal antagonist (Trichoderma harzianum). ZnONPs were then characterized for their size, shape, charge and composition by visual analysis, UV–visible spectrometry, X-ray diffraction (XRD), Zeta potential, transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive X-ray analysis (EDX). The TEM test confirmed that the size of the produced ZnONPs was 8–23 nm. The green synthesized ZnONPs were characterized by Fourier transform infrared spectroscopy (FTIR) studies to reveal the functional group attributed to the formation of ZnONPs. For the first time, trichogenic ZnONPs were shown to have fungicidal action against three soil–cotton pathogenic fungi in the laboratory and greenhouse. An antifungal examination was used to evaluate the bioactivity of the mycogenic ZnONPs in addition to two chemical fungicides (Moncut and Maxim XL) against three soil-borne pathogens, including Fusarium sp., Rhizoctonia solani and Macrophomina phaseolina. The findings of this study show a novel fungicidal activity in in vitro assay for complete inhibition of fungal growth of tested plant pathogenic fungi, as well as a considerable reduction in cotton seedling disease symptoms under greenhouse conditions. The formulation of a trichogenic ZnONPs form was found to increase its antifungal effect significantly. Finally, the utilization of biocontrol agents, such as T. harzianum, could be a safe strategy for the synthesis of a medium-scale of ZnONPs and employ it for fungal disease control in cotton.

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“…The peaks showed the characteristic hexagonal wurtzite structure of ZnO (JCPDS card no. 36-1451) [42]. The Wurtzite structure was prevalent because it is stable in ambient conditions.…”

Section: Discussionmentioning

confidence: 99%

Trichoderma harzianum-Mediated ZnO Nanoparticles: A Green Tool for Controlling Soil-Borne Pathogens in Cotton

Zaki

Ouf

Albarakaty

et al. 2021

JoF

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“…Moreover, when they performed charge–discharge up to 400 cycles, a power retention of 92% was observed. In another work by Silva and co-workers [ 38 ], it was reported for the first time the facile green sol-gel synthesis of ZnO NPs using whey as a chelating agent, characterizing the samples using different analytical techniques.…”

Section: Green Synthesis Methods Of Tio 2 and Zno Nanostructuresmentioning

confidence: 99%

Green Synthesis and Applications of ZnO and TiO2 Nanostructures

et al. 2021

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Over the last two decades, oxide nanostructures have been continuously evaluated and used in many technological applications. The advancement of the controlled synthesis approach to design desired morphology is a fundamental key to the discipline of material science and nanotechnology. These nanostructures can be prepared via different physical and chemical methods; however, a green and ecofriendly synthesis approach is a promising way to produce these nanostructures with desired properties with less risk of hazardous chemicals. In this regard, ZnO and TiO2 nanostructures are prominent candidates for various applications. Moreover, they are more efficient, non-toxic, and cost-effective. This review mainly focuses on the recent state-of-the-art advancements in the green synthesis approach for ZnO and TiO2 nanostructures and their applications. The first section summarizes the green synthesis approach to synthesize ZnO and TiO2 nanostructures via different routes such as solvothermal, hydrothermal, co-precipitation, and sol-gel using biological systems that are based on the principles of green chemistry. The second section demonstrates the application of ZnO and TiO2 nanostructures. The review also discusses the problems and future perspectives of green synthesis methods and the related issues posed and overlooked by the scientific community on the green approach to nanostructure oxides.

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“…The CE-ZnONPs showed scattering of X-rays in many directions that means the ZnONPs synthesized without UV irradiation were not of defined crystallinity (Figure5A). However, the XRD diffractogram observed at 2θ for the CE-UV-A-ZnONPs showed characteristic peaks at 31.75°, 34.30°, 36.05°, 47.25°, 56.75°, 62.55° and 67.90° (Figure5B), which correspond to the 100, 002, 101, 102, 110, 103 and 200 reflection planes and the hexagonal structure of ZnONPs (JCPDS 36-1451)[55]. Similarly, the XRD diffractogram of CE-UV-C-ZnONPs showed diffraction peaks at 31.65°, 34.55°, 36.10°, 47.70°, 56.45°, 63.00° and 68.1° (Figure5C), which correspond with the 100, 002, 101, 102, 110, 103, and 200 reflection planes and the hexagonal structure of ZnONPs.…”

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confidence: 99%

Effect of UV Irradiation (A and C) on Casuarina equisetifolia-Mediated Biosynthesis and Characterization of Antimicrobial and Anticancer Activity of Biocompatible Zinc Oxide Nanoparticles

et al. 2021

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The green synthesis of nanoparticles has emerged as a simple, safe, sustainable, reliable and eco-friendly protocol. Among different types of NPs, green-synthesized zinc oxide NPs (ZnONPs) show various promising biological uses due to their interesting magnetic, electrical, optical and chemical characteristics. Keeping in view the dependence of the therapeutic efficacy of NPs on their physico-chemical characteristics, the green synthesis of ZnONPs using Casuarina equisetifolia leaf extract under UV-A and UV-C light was carried out in this study. UV-irradiation helped to control the size and morphology of ZnONPs by exciting the electrons in the photoactive compounds of plant extracts to enhance the bio-reduction of ZnO into ZnONPs. C. equisetifolia leaf extract was found enriched with phenolic (2.47 ± 0.12 mg GAE/g DW) and flavonoid content (0.88 ± 0.28 mg QE/g DW) contributing to its 74.33% free-radical scavenging activity. FTIR spectra showed the involvement of polyphenols in the bio-reduction, stabilization and capping of ZnONPs. Moreover, SEM-EDX and XRD analyses showed great potential of UV-C light in yielding smaller (34–39 nm) oval-shaped ZnONPs, whereas UV-A irradiation resulted in the formation of fairly spherical 67–71 nm ZnONPs and control ZnONPs were of mixed shape and even larger size (84–89 nm). Green-synthesized ZnONPs, notably CE-UV-C-ZnONPs, showed promising anti-bacterial activities against Bacillus subtilis, Pseudomonas fluorescens and Pseudomonas aeruginosa. Moreover, ZnONPs also enhanced ROS production which led to a significant loss of mitochondrial membrane potential and activated caspase-3 gene expression and caspase-3/7 activity in human hepatocellular carcinoma (HepG2) cells. CE-UV-C-ZnONP treatment reduced HepG2 cell viability to as low as 36.97% owing to their unique shape and smaller size. Lastly, ZnONPs were found to be highly biocompatible towards brine shrimp and human red blood cells suggesting their bio-safe nature. This research study sheds light on the plausible role of UV radiation in the green synthesis of ZnONPs with reasonable control over their size and morphology, thus improving their biological efficacy.

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Green synthesis of ZnO nanoparticles using whey as an effective chelating agent

Cited by 44 publications

References 17 publications

Trichoderma harzianum-Mediated ZnO Nanoparticles: A Green Tool for Controlling Soil-Borne Pathogens in Cotton

Trichoderma harzianum-Mediated ZnO Nanoparticles: A Green Tool for Controlling Soil-Borne Pathogens in Cotton

Green Synthesis and Applications of ZnO and TiO2 Nanostructures

Effect of UV Irradiation (A and C) on Casuarina equisetifolia-Mediated Biosynthesis and Characterization of Antimicrobial and Anticancer Activity of Biocompatible Zinc Oxide Nanoparticles

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