Green synthesis of ZnO nanoparticles using orange fruit peel extract for antibacterial activities

Thi, Tu Uyen Doan; Nguyen, Trung Thoai; Thi, Y Dang; Ta, Hanh Kieu Thi; Phan, Bach Thang; Pham, Ngoc Kim

doi:10.1039/d0ra04926c

Cited by 266 publications

(66 citation statements)

References 38 publications

(34 reference statements)

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“…1 filter paper according to water extraction to produce more concentrated antioxidants (polyphenols and flavonoids). 5,24 Synthesis and Characterization of ZnO NPs ZnO (41.3 mg) was poured into 250 mL dd H 2 O. FSE 10 mL was added, and the solution was thoroughly mixed for 5 min in a shaking incubator. The reaction mixture was maintained at 99°C for 1 h, when the color change from light yellow to dark yellow occurs, indicating the reduction in the number of zinc ions and the synthesis of ZnO NPs.…”

Section: Fennel Seed Extractionmentioning

confidence: 99%

“…The extracted mass was 16-18% of the total mass. [22][23][24] The present study aims to combine a comfortable, safe, and cheap method as the water extraction of fennel seed with the use of the FSE to produce ZnO NPs. As far as it is to our knowledge, this study established the biological activities of biosynthesized ZnO NPs from fennel extract and their therapeutic potential for the first time.…”

Section: Introductionmentioning

confidence: 99%

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<p>Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract</p>

AlSalhi¹,

Devanesan²,

Al-Qahtani³

et al. 2020

IJN

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To study the cytotoxic evaluation, antimicrobial and confocal analysis of zinc oxide nanoparticles (ZnO NPs) obtained from a novel plant product fennel (Foeniculum vulgare Mill.) seed extract (FSE). Methods: ZnO NPs were analyzed using UV-Vis spectroscopy, XRD, FTIR, TEM and EDX techniques. The MTT cell cytotoxicity assay measured the proliferation and survival of MCF-7 cells treated at different concentrations of FSE-derived ZnO NPs. The antimicrobial activity towards pathogenic bacteria and yeast strains was investigated. Results: The UV-Vis spectra showed two peaks at 438 nm and 446 nm, confirming nanoparticle formation. The SEM morphology results showed porous ranging from 23-51 nm. The antitumor activity value (IC 50) was at 50 µg/mL and 100 µg/mL. Besides, morphological changes of MCF-7, cells treated at different concentrations of FSE of ZnO NPs were observed in cell cultures transfected with a transient pCMV6-XL4-GFP-expressing vector containing C-terminal domain GFP-tagged proteins, which resulted in an apoptotic effect. Antimicrobial IZ ranged up No Inhibition to 18.00 ± 0.4. The IZ revealed at the highest concentration was E. faecium VRE and yeast Cryptococcus sp. (18.00 ± 0.4. mm), followed by S. aureus (17.00 ± 0.2 mm) and P. aeruginosa and the yeast C. parapsilosis (16 ± 0.4 mm). The IZ was equal to that caused by the nystatin to Cryptococcus sp., which was significantly highest than ampicillin treatments of S. aureus, P. aeruginosa, C. albicans, and C. parapsilosis. The MIC value of the FSE-derived ZnO NPs tested against E.faecium and C. albicans was 6.00 µg/mL (E. faecium and C. albicans). It was 32.00 µg/mL (S. aureus, S. typhimurium and Cryptococcus sp.), 64.00 µg/mL (P. aeruginosa), and 128 µg/mL (C. parapsilosis). Conclusion: As far as it is to our knowledge, this study established, for the first time, the biological activities of biosynthesized ZnO NPs from FSE and their synergistic therapeutic potential.

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Section: Fennel Seed Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

<p>Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract</p>

AlSalhi¹,

Devanesan²,

Al-Qahtani³

et al. 2020

IJN

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“…The effects of pH and annealing temperature on the particle size of ZnO during synthesis were effectively studied on Doan et al study [ 50 ]. The ZnO NPs that are used to test the antibacterial activity against S. aureus and E. coli are synthesized using orange fruit peel extract.…”

Section: Introductionmentioning

confidence: 99%

A Review on Enhancing the Antibacterial Activity of ZnO: Mechanisms and Microscopic Investigation

et al. 2020

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Metal oxide nanomaterials are one of the preferences as antibacterial active materials. Due to its distinctive electronic configuration and suitable properties, ZnO is one of the novel antibacterial active materials. Nowadays, researchers are making a serious effort to improve the antibacterial activities of ZnO by forming a composite with the same/different bandgap semiconductor materials and doping of ions. Applying capping agents such as polymers and plant extract that control the morphology and size of the nanomaterials and optimizing different conditions also enhance the antibacterial activity. Forming a nanocomposite and doping reduces the electron/hole recombination, increases the surface area to volume ratio, and also improves the stability towards dissolution and corrosion. The release of antimicrobial ions, electrostatic interaction, reactive oxygen species (ROS) generations are the crucial antibacterial activity mechanism. This review also presents a detailed discussion of the antibacterial activity improvement of ZnO by forming a composite, doping, and optimizing different conditions. The morphological analysis using scanning electron microscopy, field emission-scanning electron microscopy, field-emission transmission electron microscopy, fluorescence microscopy, and confocal microscopy can confirm the antibacterial activity and also supports for developing a satisfactory mechanism. Graphical abstract Graphical abstract showing the metal oxides antibacterial mechanism and the fluorescence and scanning electron microscopic images.

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“…Comparing with chemical reagents, toxic free and eco-friendly plant extracts are cost competitive agents for preparing ZnO NPs [216][217][218][219][220]. The plant-based organic compounds are abundant and renewable bioresources.…”

Section: Biosynthesismentioning

confidence: 99%

“…They also act as a capping agent/stabilizer for forming ZnO NPs. In some cases, hydroxyl and carboxylic groups of citrus fruit peel extracts (e.g., flavonol and carotenoid) serve as natural ligand agents for zinc ions from zinc salt precursor [217][218][219]. For instance, Fumar et al proposed the reactions between functional groups of grapefruit peel extract and zinc ions from zinc sulfate precursor in forming a zinc-ligand complex as given in Figure 14a [217].…”

Section: Biosynthesismentioning

confidence: 99%

Recent Advances in Zinc Oxide Nanostructures with Antimicrobial Activities

Liao

Tjong

2020

IJMS

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This article reviews the recent developments in the synthesis, antibacterial activity, and visible-light photocatalytic bacterial inactivation of nano-zinc oxide. Polycrystalline wurtzite ZnO nanostructures with a hexagonal lattice having different shapes can be synthesized by means of vapor-, liquid-, and solid-phase processing techniques. Among these, ZnO hierarchical nanostructures prepared from the liquid phase route are commonly used for antimicrobial activity. In particular, plant extract-mediated biosynthesis is a single step process for preparing nano-ZnO without using surfactants and toxic chemicals. The phytochemical molecules of natural plant extracts are attractive agents for reducing and stabilizing zinc ions of zinc salt precursors to form green ZnO nanostructures. The peel extracts of certain citrus fruits like grapefruits, lemons and oranges, acting as excellent chelating agents for zinc ions. Furthermore, phytochemicals of the plant extracts capped on ZnO nanomaterials are very effective for killing various bacterial strains, leading to low minimum inhibitory concentration (MIC) values. Bioactive phytocompounds from green ZnO also inhibit hemolysis of Staphylococcus aureus infected red blood cells and inflammatory activity of mammalian immune system. In general, three mechanisms have been adopted to explain bactericidal activity of ZnO nanomaterials, including direct contact killing, reactive oxygen species (ROS) production, and released zinc ion inactivation. These toxic effects lead to the destruction of bacterial membrane, denaturation of enzyme, inhibition of cellular respiration and deoxyribonucleic acid replication, causing leakage of the cytoplasmic content and eventual cell death. Meanwhile, antimicrobial activity of doped and modified ZnO nanomaterials under visible light can be attributed to photogeneration of ROS on their surfaces. Thus particular attention is paid to the design and synthesis of visible light-activated ZnO photocatalysts with antibacterial properties

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Green synthesis of ZnO nanoparticles using orange fruit peel extract for antibacterial activities

Abstract: This paper presents an efficient, environmentally friendly, and simple approach for the green synthesis of ZnO nanoparticles (ZnO NPs) using orange fruit peel extract.

Cited by 266 publications

References 38 publications

<p>Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract</p>

<p>Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract</p>

A Review on Enhancing the Antibacterial Activity of ZnO: Mechanisms and Microscopic Investigation

Recent Advances in Zinc Oxide Nanostructures with Antimicrobial Activities

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