Green synthesis of zinc oxide nanoparticles using Sea Lavender (Limonium pruinosum L. Chaz.) extract: characterization, evaluation of anti-skin cancer, antimicrobial and antioxidant potentials

Naiel, Bassant; Fawzy, Manal; Halmy, Marwa Waseem A.; Mahmoud, Alaa El Din

doi:10.1038/s41598-022-24805-2

Cited by 72 publications

(42 citation statements)

References 87 publications

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“…The SEM analysis was conducted to find the size and surface morphology of pumpkin seed-derived ZnO NP at different magnification levels and the presence of certain elemental composition in ZnO NP was further confirmed by using EDX analysis. where a similar result was also shown in literature (Naiel et al, 2022).…”

Section: Scanning Electron Microscope (Sem) and Energy Dispersive X-r...supporting

confidence: 90%

Evaluation of the cytotoxicity, antioxidant activity, and molecular docking of biogenic zinc oxide nanoparticles derived from pumpkin seeds

Kadir,

Murugan,

Khan

et al. 2023

Microscopy Res & Technique

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This study aimed to investigate the characterization of zinc oxide nanoparticles (ZnONPs) produced from Cucurbita pepo L. (pumpkin seeds) and their selective cytotoxic effectiveness on human colon cancer cells (HCT 116) and African Green Monkey Kidney, Vero cells. The study also investigated the antioxidant activity of ZnONPs. The study also examined ZnONPs' antioxidant properties. This was motivated by the limited research on the comparative cytotoxic effects of ZnO NPs on normal and HCT116 cells. The ZnO NPs were characterized using Fourier‐transform infrared spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Transmission Electron Microscope/Selected Area Electron Diffraction (TEM/SAED), and Scanning Electron Microscope‐Energy Dispersive X‐ray (SEM–EDX) for determination of chemical fingerprinting, heat stability, size, and morphology of the elements, respectively. Based on the results, ZnO NPs from pumpkins were found to be less than 5 μm and agglomerates in nature. Furthermore, the ZnO NPs fingerprinting and SEM–EDX element analysis were similar to previous literature, suggesting the sample was proven as ZnO NPs. The ZnO NPs also stable at a temperature of 380°C indicating that the green material is quite robust at 60–400°C. The cell viability of Vero cells and HCT 116 cell line were measured at two different time points (24 and 48 h) to assess the cytotoxicity effects of ZnO NP on these cells using AlamarBlue assay. Cytotoxic results have shown that ZnO NPs did not inhibit Vero cells but were slightly toxic to cancer cells, with a dose–response curve IC50 = ~409.7 μg/mL. This green synthesis of ZnO NPs was found to be non‐toxic to normal cells but has a slight cytotoxicity effect on HCT 116 cells. A theoretical study used molecular docking to investigate nanoparticle interaction with cyclin‐dependent kinase 2 (CDK2), exploring its mechanism in inhibiting CDK2's role in cancer. Further study should be carried out to determine suitable concentrations for cytotoxicity studies. Additionally, DPPH has a significant antioxidant capacity, with an IC50 of 142.857 μg/mL.Research Highlights Pumpkin seed extracts facilitated a rapid, high‐yielding, and environmentally friendly synthesis of ZnO nanoparticles. Spectrophotometric analysis was used to investigate the optical properties, scalability, size, shape, dispersity, and stability of ZnO NPs. The cytotoxicity of ZnO NPs on Vero and HCT 116 cells was assessed, showing no inhibition of Vero cells and cytotoxicity of cancer cells. The DPPH assay was also used to investigate the antioxidant potential of biogenic nanoparticles. A molecular docking study was performed to investigate the interaction of ZnO NPs with CDK2 and to explore the mechanism by which they inhibit CDK2's role in cancer.

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Section: Scanning Electron Microscope (Sem) and Energy Dispersive X-r...supporting

confidence: 90%

Evaluation of the cytotoxicity, antioxidant activity, and molecular docking of biogenic zinc oxide nanoparticles derived from pumpkin seeds

Kadir,

Murugan,

Khan

et al. 2023

Microscopy Res & Technique

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“…Our results agree with that of a prior report which specified that the bio-prepared ZnO-NPs produced Limonium pruinosum L. Chaz. extract and recorded a relative IC 50 of 568.59 μg/mL against the WI-38 cell line [ 57 ]. The US Food and Drug Administration recommends that zinc oxide nanoparticles be considered safe (i.e., generally recognized as safe) [ 58 ].…”

Section: Resultsmentioning

confidence: 99%

Synergistic Anticandidal Activities of Greenly Synthesized ZnO Nanomaterials with Commercial Antifungal Agents against Candidal Infections

et al. 2023

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The high occurrence of mycological resistance to conventional antifungal agents results in significant illness and death rates among immunodeficient patients. In addition, the underprivileged therapeutic results of conventional antifungal agents, besides the potential toxicity resulting from long term therapy necessitate the fabrication of efficient antimicrobial combinations. Hence, the objective of the present investigation is to synthesize, characterize and investigate the anticandidal action of green zinc oxide nanoparticles (ZnO-NPs) formulated using Camellia sinensis leaf extract against three candidal pathogens. The eco-friendly synthesized ZnO-NPs were characterized utilizing different physicochemical methods and their anticandidal potency was tested utilizing a disk diffusion assay. In this setting, the size of the biofabricated ZnO-NPs was detected using transmission electron microscope (TEM) micrographs, recording an average particle size of 19.380 ± 2.14 nm. In addition, zeta potential analysis revealed that the ZnO-NPs surface charge was −4.72 mV. The biogenic ZnO-NPs reveal the highest anticandidal activity against the C. tropicalis strain, demonstrating relative suppressive zones measured at 35.16 ± 0.13 and 37.87 ± 0.24 mm in diameter for ZnO-NPs concentrations of 50 and 100 μg/disk, respectively. Excitingly, Candida glabrata showed a high susceptibility to the biofabricated ZnO nanomaterials at both ZnO-NPs’ concentrations (50 and 100 μg/disk) compared to the control. Moreover, the biosynthesized ZnO-NPs revealed potential synergistic effectiveness with nystatin and terbinafine antifungal agents against the concerned strains. The maximum synergistic efficiency was noticed against the C. glabrata strain, demonstrating relative synergistic percentages of 23.02 and 45.9%, respectively. The biogenic ZnO-NPs revealed no hemolytic activity against human erythrocytes revealing their biosafety and hemocompatibility. Finally, the high anticandidal effectiveness of biogenic ZnO-NPs against the concerned candidal pathogens, as well as potential synergistic patterns with conventional antifungal agents such as nystatin and terbinafine, emphasize the prospective application of these combinations for the fabrication of biocompatible and highly efficient antifungal agents.

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“…Obtaining AgNPs is of interest not only as a method and assessment of the level of reducing power, but also as an independent biotechnological process of creating a product (nanoparticles), which can find wide practical applications due to having anticancer effect, antibacterial, antioxidant, anti-inflammatory and anti-hyperglycemic activity [18][19][20][21][22][23][24][25], as well as anti-quorum sensing potential [26]. Advances of such "green" silver, zink, gold, platinum, nickel, aluminum and iron nanoparticles are widely researched [27][28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Optical Characteristics of Silver Nanoparticles Obtained Using Artemisia tilesii Ledeb. "Hairy" Root Extracts With High Flavonoid Content

Bohdanovych

Матвєєва

2023

Innov Biosyst Bioeng

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Background. "Hairy" roots can be used for production of plant-derived secondary metabolites, such as flavonoids, which have antioxidant and reducing properties. It seems promising to use the process of silver nanoparticles formation as a method of determining the level of reducing power. This approach allows simultaneously to obtain nanoparticles with different biological activity and evaluate the reducing potential of different plants. Objective. The aim of the study was to determine the dependence of optical properties (ultraviolet/visible spectroscopy, UV-Vis spectra) of solutions of silver nanoparticles obtained using wormwood "hairy" root extracts on the content of flavonoids as compounds with reducing activity. Methods. Four Artemisia tilesii “hairy” root lines from the collection of the Institute of Cell Biology and Genetic Engineering NAS of Ukraine were grown for five weeks in liquid 1/2 MS medium with 20 g/l sucrose. "Hairy" roots were homogenized in 70% ethanol, the extracts were centrifuged, total flavonoid content was determined, and extracts were used for the silver nanoparticles preparation via reduction of Ag+ to Ag0 from AgNO3. UV-Vis spectra at 300–600 nm range were recorded right after colloid solution preparation, in five, and in nine days. Results. Total flavonoid content varied from 4.01 ± 0.39 to 15.37 ± 1.08 mg RE/g FW. The UV-Vis spectra curves of absorption increased with the course of time, mostly from day 0 to day 5. At this period, absorption at 370–500 nm wavelength increased almost twofold. The peak absorption of all samples was detected at 440 nm, and the maximum values at the wavelength of 440 nm correlated with the content of flavonoids. This correlation did not change over time. Conclusions. The optical properties of silver nanoparticles colloid solutions obtained using A. tilesii "hairy" root extracts correlated with the total flavonoid content of the samples. Even though the absorbance of the colloid solutions increased with time, those increases correlated with flavonoid content as well. The determination of the optical properties of AgNPs colloid solution can be used as a convenient way of quickly comparing the reducing ability of extracts both right after the formation of a colloidal solution and after some time of its storage.

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Green synthesis of zinc oxide nanoparticles using Sea Lavender (Limonium pruinosum L. Chaz.) extract: characterization, evaluation of anti-skin cancer, antimicrobial and antioxidant potentials

Cited by 72 publications

References 87 publications

Evaluation of the cytotoxicity, antioxidant activity, and molecular docking of biogenic zinc oxide nanoparticles derived from pumpkin seeds

Evaluation of the cytotoxicity, antioxidant activity, and molecular docking of biogenic zinc oxide nanoparticles derived from pumpkin seeds

Synergistic Anticandidal Activities of Greenly Synthesized ZnO Nanomaterials with Commercial Antifungal Agents against Candidal Infections

Optical Characteristics of Silver Nanoparticles Obtained Using Artemisia tilesii Ledeb. "Hairy" Root Extracts With High Flavonoid Content

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