Green synthesis of silver nanoparticles using  Capsicum frutescence  and its intensified activity against  E. coli

Shankar, Thangaraj; Karthiga, Perumal; Swarnalatha, K.; Rajkumar, Kalaiyar

doi:10.1016/j.reffit.2017.01.004

Cited by 52 publications

(36 citation statements)

References 21 publications

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“…We can conclude that using more than one plant extract increases the antimicrobial activity of the AgNPs. Further, the activity of AgNPs against the gram-positive bacteria S. aureus was extraordinary compared to the previous reports in the literature [16,19]. Synergistic antibacterial effects of AgNPs and naturally occurring compounds in capsicum, garlic and ginger possibly resulted in higher bactericidal activity.…”

Section: In Vitro Antimicrobial Activity Of Agnpsmentioning

confidence: 74%

“…All the selected strains were inoculated in Mueller Hinton Broth (MHB) cultures. A loopful of pathogenic cells from the stock cultures were transferred to test tubes containing 10 mL MHB and incubated at 37 • C for 24 h. Agar well diffusion method was carried out according to the standard method to assess the antibacterial activity of the synthesized AgNPs [16]. The standard inoculum using microbial suspensions containing 10 5…”

Section: In Vitro Antimicrobial Activity Of Agnpsmentioning

confidence: 99%

“…Capsaicin alkaloid and antioxidants of the capsicum extract act as reducing and stabilizing agents during the nanoparticle formation. The reduction of silver ions and the growth of nanoparticles happened within one hour due to excitation of surface plasmon vibrations in the nanoparticles [16]. Increasing the extract concentration above 5% v/v led to formation of a grayish dispersion, indicating the formation of silver colloid due to particle aggregation .…”

Section: Uv-vis Absorbance Spectrophotometrymentioning

confidence: 99%

“…The band at 1017 cm −1 belongs to C-O stretching of ethers in plant extract. Other phenolic and flavonoid constituents could react with silver ions during the formation of AgNPs [16].…”

Section: Fourier-transform Infrared (Ftir) Spectroscopymentioning

confidence: 99%

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Green Synthesis of Potent Antimicrobial Silver Nanoparticles Using Different Plant Extracts and Their Mixtures

et al. 2019

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Nano-sized metals have been introduced as a promising solution for microbial resistance to antimicrobial agents. Silver nanoparticles (AgNPs) have been proven to possess good antimicrobial activity. Green synthesis of AgNPs has been reported as safe, low cost and ecofriendly. This methodology uses extracts originating from different plants to reduce silver ions from AgNO 3 into nano-sized particles. In this study, extracts of several plants including ginger, garlic, capsicum and their mixtures were successfully used to produce AgNPs. Numerous spectroscopic, light scattering and microscopic techniques were employed to characterize the synthesized AgNPs. Agar well diffusion assay was performed to investigate the antimicrobial activity of AgNPs. The biosynthesized AgNPs have spherical shape with a size range of 20-70 nm. Garlic extract, pure or in mixture with ginger extract, generated AgNPs of the smallest size. The presence of the plant-origin capping agents surrounding AgNPs was proven by Fourier-transform infrared spectroscopy. The AgNPs, at a concentration of 50 µg/mL, demonstrated potent antimicrobial activity against Staphyloccocus aureus, Escherichia coli and Candida albicans as indicated by the zones of inhibitions. Our results revealed that AgNPs having potent antimicrobial activity could be prepared using different pure plant extracts and their mixtures.

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Section: In Vitro Antimicrobial Activity Of Agnpsmentioning

confidence: 74%

Section: In Vitro Antimicrobial Activity Of Agnpsmentioning

confidence: 99%

Section: Uv-vis Absorbance Spectrophotometrymentioning

confidence: 99%

“…The band at 1017 cm −1 belongs to C-O stretching of ethers in plant extract. Other phenolic and flavonoid constituents could react with silver ions during the formation of AgNPs [16].…”

Section: Fourier-transform Infrared (Ftir) Spectroscopymentioning

confidence: 99%

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Green Synthesis of Potent Antimicrobial Silver Nanoparticles Using Different Plant Extracts and Their Mixtures

et al. 2019

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“…The aqueous extract of C. cassia was prepared by adding a fine powder of the plant (10 g) to 300 mL of double-distilled water, which was allowed to boil for 10 minutes [16]. The resulting mixture was filtered and stored in a refrigerator at 4°C until analyzed.…”

Section: Preparation Of Aqueous Plants Extractmentioning

confidence: 99%

Histological and biochemical effects of Cinnamomum cassia nanoparticles in kidneys of diabetic Sprague-Dawley rats

Kouame

Akang

Moodley

et al. 2019

Bosn J of Basic Med Sci

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This study investigated the antidiabetic activity of Cinnamomum cassia (C. cassia, Cc) silver nanoparticles (CcAgNPS) and effects of C. cassia on the kidneys of rats with induced type 2 diabetes. Twenty-four Sprague-Dawley rats weighing 250 ± 20 g were induced with diabetes by intraperitoneal injection of streptozotocin (STZ, 60 mg/kg). Animals were randomly assigned to one of four groups (n = 6) and treated for eight weeks with normal saline (control, group A), 5 mg/kg of CcAgNPs (group B), 10 mg/kg of CcAgNPs (group C), or 200 mg/kg of Cc (group D). Body weight and fasting blood glucose (FBG) was measured weekly and fortnightly, respectively. At the end of experiments, animals were euthanized, blood and kidney tissue samples were collected for biochemistry (oxidative stress markers and renal function parameters) and kidneys were harvested for histology (PAS and HE staining). Body weight was significantly higher in group B and C vs. control (p < 0.05), while no significant differences were observed in the kidney-to-body weight ratio between groups. FBG, glutathione, malondialdehyde, alanine aminotransferase, aspartate aminotransferase, serum urea and creatinine were significantly lower in group B, C and/or D vs. control (all p < 0.05). In group A, severe distortion of the glomerular network was observed, marked by the loss of capsular integrity, thickened basement membrane, tubular cells with pyknotic nuclei, vacuolization, and interstitial space with infiltrations. These adverse effects were mitigated by 5 mg/kg and 10 mg/kg of CcAgNPs. Our study confirms structural and functional damage to kidneys caused by diabetes. CcAgNPs have a regenerative potential in diabetes-induced kidney damage and may be used as an antidiabetic agent.

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Green synthesis of silver nanoparticles by using eugenol and evaluation of antimicrobial potential

Tekin

Guldu

Derviş

et al. 2019

Applied Organom Chemis

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The importance of green synthesis was revealed with advantages such as: eliminating the use of expensive chemicals; consume less energy; and generate environmentally benign products. With this aim, silver nanoparticles (AgNPs) were synthesized by using isolated eugenol from clove extract. Its antimicrobial potential was determined on three different microorganisms. Clove was extracted and eugenol was isolated from this extract. Green synthesis was performed and an anti‐microbial study was performed. All extraction and isolation analyses were performed by high‐performance liquid chromatography (HPLC); identification and confirmation were achieved using liquid chromatography–mass spectrometry (LC–MS); and scanning electron microscopy was used for characterization. Both HPLC and LC–MS analyses showed that eugenol obtained purely synthesized AgNPs and 20‐25‐nm‐sized and homogeneous shaped particles seen in images. The antimicrobial effects of AgNPs at eight concentrations were determinated against Staphylococcus aureus, Escherichia coli and Candida albicans, and maximum inhibition zone diameters were found as 2.6 cm, 2.4 cm and 1.5 cm, respectively. The results of the antimicrobial study showed that eugenol as a biological material brought higher antimicrobial effect to AgNPs in comparison to the other materials found in the literature.

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Green synthesis of silver nanoparticles using Capsicum frutescence and its intensified activity against E. coli

Cited by 52 publications

References 21 publications

Green Synthesis of Potent Antimicrobial Silver Nanoparticles Using Different Plant Extracts and Their Mixtures

Green Synthesis of Potent Antimicrobial Silver Nanoparticles Using Different Plant Extracts and Their Mixtures

Histological and biochemical effects of Cinnamomum cassia nanoparticles in kidneys of diabetic Sprague-Dawley rats

Green synthesis of silver nanoparticles by using eugenol and evaluation of antimicrobial potential

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