Green synthesis of silver nanoparticles using Alysicarpus monilifer leaf extract and its antibacterial activity against MRSA and CoNS isolates in HIV patients

Abstract

The emergence of multi-drug-resistant microorganisms in hospital environments is a global public health problem and threat to everyone, especially HIV-infected patients.Methicillin-resistant Staphylococcus aureus (MRSA) and coagulase-negative Staphylococci (CoNS) are the major causative agents associated with morbidity and mortality in HIV patients. Therefore, control of MRSA and CoNS-related infections in HIV patients is a worldwide concern. To investigate novel, potent, and cost-effective therapeutic approaches, the current study reports a simple and rapid synthesis of silver nanoparticles (AgNPs) using aqueous leaf extract of Alysicarpus monilifer and its antibacterial efficacy against multi-drug-resistant MRSA and CoNS isolates from HIV patients. The greensynthesized AgNPs were characterized using ultraviolet-visible spectroscopy, transmission electron microscopy, energy dispersive X-ray analysis, selected area electron diffraction pattern, X-ray diffraction patterns, and Fourier transform infrared spectroscopy. Stable, well-defined AgNPs, mostly spherical in shape with mean size of 15 ± 2 nm, were obtained within an hour. Moreover, green synthesized AgNPs revealed significant dose-dependent antibacterial action against MRSA and CoNS isolates. This study concludes that biogenic AgNPs have demonstrated to be potent antibacterial agents in comparison with conventional antibiotics.

Green synthesis of silver nanoparticles using water extract of Salvia leriifolia: Antibacterial studies and applications as catalysts in the electrochemical detection of nitrite

“…On the other hand, the comparison between FT‐IR spectrum of the leaf extract and nanoparticles shows that there is a considerable similarity in their spectra (Figure ). According to Su et al study on Salvia miltiorrhiza , there is no difference between the FT‐IR spectrum of the plant and synthesized nanoparticles . For the FT‐IR spectrum of Pogostemon benghalensis nanoparticles, the strong IR bands were observed at 3382, 2922, 1614, and 1384 cm −1 which refers to ‐OH and aliphatic ‐C‐H stretching, C = C stretching and ‐C‐O stretching, respectively, which are found as functional groups in most natural products …”

“…It can be obviously seen that the S.l ‐AgNPs are distributed uniformly and having an average size of 27.18 nm in diameter. To further reveal, EDX of S.l ‐AgNPs was also analyzed as shown in Figure b, it is clear that the element Ag was present . On the other hand, nanoparticles aggregation phenomenon is an important and fundamental subject when associated with many applications of NPs, such as in optical technological field and sensing process.…”

“…Arokiyaraj et al reported the synthesized AgNPs that was obtained using the floral extract of Chrysanthemum indicum exhibited higher fatality than the plant extract in larvicidal and pupicidal activity against the mosquito larvae and pupae of Anopheles stephensi . According to previous studies, the formation of free radicals by nanoparticles, membrane permeability to cause cellular leakage, interaction with protein, DNA, or enzymes to inactive microorganisms are the reasons of AgNPs ability to prevent cell growth and replication ability of the microorganisms.…”

Synthesis of silver nanoparticles using Peganum harmala extract as a green route

“…Its formation was also confirmed by UV-vis spectroscopy. The color change is caused by coherent excitation of the SPR in metallic nanoparticles (41).…”

Electrochemical properties of biogenic silver nanoparticles synthesized using Hagenia abyssinica (Brace) JF. Gmel. medicinal plant leaf extract

“…Many plants parts or whole plants have been used for the green synthesis of Ag NPs due to the presence of large number of bioactive compounds in plants. The extracts of plants and various parts of plants, Aloe fleurentiniorum plant [3], Coffea arabica seed [4], Jatropa Gossypifolia and Jatropa Glandulifera leaf [5], M. balbisiana (banana), A. indica (neem) and O. tenuiflorum (black tulsi) leaves [6], Alysicarpus monilifer leaves [7], Atrocarpus altilis leaves [8], Pimpinella anisum seeds [9] Aloe Vera plant [10], Azadirachta indica [11], olive leaf [12], banana peels 9 [13], Skimmia laureola leaves [14], Ziziphora tenuior (Zt) plant [15], Acacia rigidula leaves [16], R. officinalis, U. dioica and V. vitis-idaea [17] and Pistacia atlantica leaves [18] have been efficiently applied for this purpose.…”