Green Synthesis of Silver Nanoparticles via <i>Phormidium</i> sp. nov. (Cyanophyceae): Amelioration, Characterization and Assessment of the Antibacterial Potential Against Methicillin Resistant <i>Staphylococcus aureus</i>

El-Semary, Nermin A.; Mabrouk, Mona I.; Faraag, Ahmed Hassan Ibrahim; Kilany, Mona; Omran, Shymaa H.; Ghramh, Hamed A.; Alshehri, A.M.; Ibrahim, Essam H.; Morsy, Kareem; El‐kott, Attalla F.; Sayed, M. A.; Sayed, Yousef El

doi:10.1166/sam.2021.3863

Cited by 4 publications

(3 citation statements)

References 41 publications

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“…against multi-drug resistant Staphylococcus aureus (MRSA). Similarly, El- Semary et al (2021) showed the strong antimicrobial activity of silver nanoparticles from a filamentous cyanobacterium. Cyanobacteria grow rapidly in relatively inexpensive growth media.…”

Section: Introductionmentioning

confidence: 95%

Nitrogen-fixing Cyanothece sp. as a mixotroph and silver nanoparticle synthesizer: a multitasking exceptional cyanobacterium

Semary¹

2022

Braz. J. Biol

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To investigate the best carbon source for mixotrophic growth of Cyanothece sp. This cyanobacterium is also used as a source of biogenic silver nanoparticles. The study also investigates the antimicrobial activity of nanoparticles (both gold and silver) biosynthesized by this cyanobacterium. Those particles are tested solely and in combination as antimicrobial agents against two farm bacteria commonly found in Al Ahsa. Soil extract was prepared from pesticide-free soil whereas dextrose was prepared in 6 mM concentration and the cyanobacterial culture cell density was determined after two weeks. Cultures of Cyanothece sp. were incubated with silver nitrate until turning brown. The external solution of culture was analyzed using chemical analyses including UV- visible and FTIR to confirm nano silver formation. Previously-biosynthesized nano gold particles by Cyanothece sp. were used solely and in combination with newly biosynthesized nano silver particles for antimicrobial bioassay against the two farm bacterial pathogens. The best mixotrophic cyanobacterial growth was obtained for soil extract followed by dextrose which were significantly different from control. The synthesis of nanoparticles using this cyanobacterium was confirmed using UV-visible light spectrophotometry which detected the characteristic surface plasmon resonance peak in the range of 410-450 nm and the FTIR spectroscopy which showed the characteristic silver nanoparticles peak at 3.297 cm-1 which overlaps with -OH- in addition to the other functional groups associated with nano silver particles detected at 2,927, 1,631 and 1,383 cm−1. Silver nanoparticles showed the strong antimicrobial effect against both pathogens Staphylococcus aureus (MRSA) and Staphylococcus warneri with inhibition zone diameter 1.3 cm for both followed by the combination of silver and gold nanoparticles. Soil extract is a natural medium rich in all types of organic and inorganic nutrients which enhance algal mixotrophic growth. Biosynthesized silver nanoparticles showed the strongest antibacterial action against both pathogens most likely due to its ease of penetration, interaction with cellular components, generation of reactive oxygen species and induction of oxidative stress leading to bacterial death.

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Section: Introductionmentioning

confidence: 95%

Nitrogen-fixing Cyanothece sp. as a mixotroph and silver nanoparticle synthesizer: a multitasking exceptional cyanobacterium

Semary¹

2022

Braz. J. Biol

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“…Several bacteria have been reported for the biosynthesis of AgNPs, including Microvirga rosea [ 22 ], Bacillus sonorensis [ 23 ], Terrabacter humi [ 24 ], etc. There are many recent reports regarding the potent antimicrobial effect of biosynthesized AgNPs against human pathogens [ 25 – 27 ]. Due to the strong antimicrobial action of AgNPs, they are used in the development of footwear, cosmetics, wound dressings and other medical devices [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Bacterial mediated green synthesis of silver nanoparticles and their antibacterial and antifungal activities against drug-resistant pathogens

Huq,

Khan,

Alshehri

et al. 2023

R. Soc. open sci.

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In the healthcare sector, the production of bioactive silver nanoparticles (AgNPs) with antimicrobial properties is of great importance. In this study, a novel bacterial strain, Paenibacillus sp. MAHUQ-63, was identified as a potential candidate for facile and rapid biosynthesis of AgNPs. The synthesized AgNPs were used to control the growth of human pathogens, Salmonella Enteritidis and Candida albicans . The bacterial culture supernatant was used to synthesize the nanoparticles (NPs). Field emission transmission electron microscope examination showed spherical-shaped NPs with 15–55 nm in size. Fourier transform-infrared analysis identified various functional groups. The synthesized AgNPs demonstrated remarkable activity against S. Enteritidis and C. albicans . The zones of inhibition for 100 µl (0.5 mg ml −1 ) of AgNPs against S. Enteritidis and C. albicans were 18.0 ± 1.0 and 19.5 ± 1.3 mm, respectively. The minimum inhibitory concentrations were 25.0 and 12.5 µg ml −1 against S. Enteritidis and C. albicans , respectively. Additionally, the minimum bactericidal concentrations were 25.0 µg ml −1 against both pathogenic microbes. The field emission scanning electron microscopy analysis showed that the treatment of AgNPs caused morphological and structural damage to both S. Enteritidis and C. albicans . Therefore, these AgNPs can be used as a new and effective antimicrobial agent.

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“…Due to the antibacterial action of silver nanoparticles, they are incorporated in footwear, cosmetics, wound dressings and plastics [ 11 ]. The antimicrobial effect of silver nanoparticles, biosynthesized by algae against human bacterial pathogens has been previously reported [ 14 , 15 ]. The cyanobacterium under study is unicellular, photosynthetic prokaryotic microorganism.…”

Section: Introductionmentioning

confidence: 99%

Multidrug-Resistant Bacterial Pathogens and Public Health: The Antimicrobial Effect of Cyanobacterial-Biosynthesized Silver Nanoparticles

Semary

Bakir

2022

Antibiotics

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Background: Cyanobacteria are considered as green nano-factories. Manipulation of the size of biogenic silver nanoparticles is needed to produce particles that suit the different applications such as the use as antibacterial agents. The present study attempts to manipulate the size of biosynthesized silver nanoparticles produced by cyanobacteria and to test the different-sized nanoparticles against pathogenic clinical bacteria. Methods: Cyanothece-like. coccoid unicellular cyanobacterium was tested for its ability to biosynthesize nanosilver particles of different sizes. A stock solution of silver nitrate was prepared from which three different concentrations were added to cyanobacterial culture. UV-visible spectroscopy and FTIR were conducted to characterize the silver nanoparticles produced in the cell free filtrate. Dynamic Light Scattering (DLS) was performed to determine the size of the nanoparticles produced at each concentration. The antimicrobial bioassays were conducted on broad host methicillin-resistant Staphylococcus aureus (MRSA), and Streptococcus sp., was conducted to detect the nanoparticle size that was most efficient as an antimicrobial agent. Results. The UV-Visible spectra showed excellent congruence of the plasmon peak characteristic of nanosilver at 450 nm for all three different concentrations, varying peak heights were recorded according to the concentration used. The FTIR of the three solutions revealed the absence of characteristic functional groups in the solution. All three concentrations showed spectra at 1636 and 2050–2290 nm indicating uniformity of composition. Moreover, DLS analysis revealed that the silver nanoparticles produced with lowest concentration of precursor AgNO3 had smallest size followed by those resulting from the higher precursor concentration. The nanoparticles resulting from highest concentration of precursor AgNO3 were the biggest in size and tending to agglomerate when their size was above 100 nm. The three types of differently-sized silver nanoparticles were used against two bacterial pathogenic strains with broad host range; MRSA-(Methicillin-resistant Staphylococcus aureus) and Streptococcus sp. The three types of nanoparticles showed antimicrobial effects with the smallest nanoparticles being the most efficient in inhibiting bacterial growth. Discussion: Nanosilver particles biosynthesized by Cyanothece-like cyanobacterium can serve as antibacterial agent against pathogens including multi-drug resistant strains. The most appropriate nanoparticle size for efficient antimicrobial activity had to be identified. Hence, size-manipulation experiment was conducted to find the most effective size of nanosilver particles. This size manipulation was achieved by controlling the amount of starting precursor. Excessive precursor material resulted in the agglomeration of the silver nanoparticles to a size greater than 100 nm. Thereby decreasing their ability to penetrate into the inner vicinity of microbial cells and consequently decreasing their antibacterial potency. Conclusion: Antibacterial nanosilver particles can be biosynthesized and their size manipulated by green synthesis. The use of biogenic nanosilver particles as small as possible is recommended to obtain effective antibacterial agents.

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Green Synthesis of Silver Nanoparticles via Phormidium sp. nov. (Cyanophyceae): Amelioration, Characterization and Assessment of the Antibacterial Potential Against Methicillin Resistant Staphylococcus aureus

Cited by 4 publications

References 41 publications

Nitrogen-fixing Cyanothece sp. as a mixotroph and silver nanoparticle synthesizer: a multitasking exceptional cyanobacterium

Nitrogen-fixing Cyanothece sp. as a mixotroph and silver nanoparticle synthesizer: a multitasking exceptional cyanobacterium

Bacterial mediated green synthesis of silver nanoparticles and their antibacterial and antifungal activities against drug-resistant pathogens

Multidrug-Resistant Bacterial Pathogens and Public Health: The Antimicrobial Effect of Cyanobacterial-Biosynthesized Silver Nanoparticles

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