Green Synthesis of Silver Nanoparticles Using Artocarpus hirsutus Seed Extract and its Antibacterial Activity

Abstract

Aim: To evaluate the antibacterial activity of Artocarpus hirsutus mediated seed extract for nanoparticle synthesis. Background: Gastro-intestinal bacteria are known for causing deadly infections in humans. They also possess multi-drug resistance and interfere with clinical treatments. Applied nanotechnology has been known to combat such infectious agents with little interference from their special attributes. Here we synthesize silver nanoparticles from Artocarpus hirsutus seed extract against two gastro-intestinal bacterial species: Enterobacter aerogenes and Listeria monocytogenes. Objective: • To collect, dried and process seeds of Artocarpus hirsutus for nanoparticle synthesis. • To evaluate the morphological interaction of silver nanoparticles with bacteria. Methodology: Artocarpus hirsutus seeds were collected, processed and further silver nanoparticles were synthesized by co-precipitation method. The synthesized nanoparticles were characterized using XRD, UV, FTIR, SEM. These nanoparticles were employed to study the antibacterial activity of nanoparticles against Enterobacter aerogenes and Listeria monocytogenes using well diffusion method. Further, morphological interaction of silver nanoparticles on bacteria were studied using SEM. Result: Silver nanoparticles were synthesized using Artocarpus hirsutus seed extract and characterization studies confirmed that silver nanoparticles were spherical in shape with 25-40 nm size. Antibacterial study exhibited better activity against Enterobacter aerogenes with maximum zone of inhibition than on Listeria monocytogenes. SEM micrographs indicated that Enterobacter aerogenes bacteria were more susceptible to silver nanoparticles due to the absence of cell wall. Also the size and charge of silver nanoparticles enables easy penetration of the bacterial cell wall. Conclusion: In this study, silver nanoparticles were synthesized using the seed extract of Artocarpus hirsutus for the first time exploiting the fact that Moraceae species have high phytonutrient content which aided in nanoparticle synthesis. This nanoparticle can be employed for large scale synthesis which when coupled with pharmaceutical industry can be used to overcome the problems associated with conventional antibiotics to treat gastro-intestinal bacteria.

Cassia fistula nutrition rich flower tea derived biotic nanoparticles synthesis, characterization and their antioxidant and anti-hyperglycaemic properties

“…The plant sources or plant source derivatives associated with nanoparticles are considered as a novel therapy due to their natural care, healthier effectiveness without any side impact, simple way of synthesis and, most importantly less cost. 3 , 4 …”

“…The plant sources or plant source derivatives associated with nanoparticles are considered as a novel therapy due to their natural care, healthier effectiveness without any side impact, simple way of synthesis and, most importantly less cost. 3,4 Nanotechnology is the most advanced technology and it has numerous applications, including several industrial fields and also in human health drug developments because of its nanosize, it has several positive approaches. Nanotechnology has been designated as the next revolution to hit many industries and it can be achieved accredited by scientists to the next step with the existing performance.…”

Anticancer mechanism of artonin E and related prenylated flavonoids from the medicinal plant Artocarpus elasticus

“…In addition, an extract of the stem bark of A. elasticus has been used as a reducing and stabilizing agent for the synthesis of silver nanoparticles (Abdullah et al 2015). The same type of antibacterial silver nanoparticles have been obtained with seed extract of A. hirsutus (Shobana et al 2020).…”

Microwave-assisted green synthesis ofDesmodium triquetrum-mediated silver nanoparticles: enhanced antibacterial, antibiofilm, and cytotoxicity activities against human breast cancer cell lines

“…14 Plant-mediated synthesis provides major benefits, including its ease of monitoring, low time consumption, low cost, and effective replacement of chemicals in the reduction process, and is the safest choice to produce silver nanoparticles. 5,15,16 Recent studies have reported that parts of the plant used for biosynthesized silver nanoparticles are roots, 17 leaves, [18][19][20] seeds 21,22 and bark. 23 Plant extracts contain many bioactive compounds (e.g.…”

“…Recent studies have reported that parts of the plant used for biosynthesized silver nanoparticles are roots, 17 leaves, 18–20 seeds 21,22 and bark. 23 Plant extracts contain many bioactive compounds ( e.g.…”