Green Synthesis of Silver Nanoparticles Using Brain Heart Infusion (BHI) Culture Medium and Evaluation of Their Antimicrobial and Anti-biofilm Activity Against Acinetobacter baumannii

Solouki, Malihe; Moniri, Rezvan; Fathizadeh, Hadis; Kashi, Fereshteh Jookar; Moosavi, Gholam Abbas; Nazari-Alam, Ali

doi:10.4274/mjima.galenos.2021.2021.50

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“…For the synthesis of AgNPs, Luria Bertani medium was used. It is considered as a new method to date ( 9 ). The advantage of this synthesis method over the chemical and physical method is the production and stability of silver nanoparticles in one step, low toxicity, and saving in time and cost.…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial effect of silver and gold nanoparticles in combination with linezolid on Enterococcus biofilm

Sabzi¹,

Moniri²,

Sehat³

et al. 2022

IJM

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Background and Objectives: In the past few years, application of new antimicrobial e.g. nanoparticles (NPs) to treat infec- tion caused by drug-resistantbacteria has increased. This study aimed to determine antimicrobial property of silver nanopar- ticles (AgNPs) and gold nanoparticles (AuNPs) in combination with linezolid on Enterococcus biofilm. Materials and Methods: A total of forty-eight isolates of Enterococcus spp. were collected and confirmed by PCR method. The synthesis of biocompatibleAgNPs was performed, then analyzed by Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Transmission ElectronMicroscopy. We carried out minimum inhibitory concen- tration (MIC) and biofilm forming capacity of AgNPs and AuNPs with linezolid. Results: Twenty-two E. faecium isolates and twenty- six E. faecalis investigated in this study. Strong biofilm formation was seen in 12 (25%) of isolates, andothers isolates (75%) formed moderate biofilm. AgNPs and Au-NPs size were 26 nm and 20 nm respectively. The MIC of AgNPs was 23.2 μg/ml, and AuNPs were 92.1 μg/ml and the lowest MIC was obtained 2 μg/ml in linezolid. Biofilmformation inhibitory activity by AuNPs + Linezolide and AgNPs + Linezolide 70 to 80 percent increased in average. Conclusion: The antibiofilm activity of AgNPs and AuNPs increased when both agents were used in combination with linezolid in comparison with each agent alone.

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Section: Discussionmentioning

confidence: 99%

“…Then it centrifuged and washed with alcohol; next, it centrifuged twice at 10,000 rpm for 20 minutes. After sonication, it dried with a vacuum drier for an overnight at 50°C ( 9 ).…”

Section: Methodsmentioning

confidence: 99%