Green Synthesis of Silver Nanoparticles Using Ocimum basilicum L. and Hibiscus sabdariffa L. Extracts and Their Antibacterial Activity in Combination with Phage ZCSE6 and Sensing Properties

Abdelsattar, Abdallah S.; Hakim, Toka A.; Rezk, Nouran; Farouk, Warda M.; Hassan, Yara Y.; Gouda, Shrouk Mohamed; El‐Shibiny, Ayman

doi:10.1007/s10904-022-02234-y

Cited by 20 publications

(10 citation statements)

References 66 publications

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“…This decreased the capacity of bacteria to develop resistance against the synthesized NPs in addition to the ongoing stress on the bacteria which were caused by AgNPs at a concentration. The findings of this study are in agreement with what was reported in a previous study 38 .…”

Section: Anti-bacterial Results Of Agnpssupporting

confidence: 94%

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The effect of different aqueous solutions ratios of Ocimum basilicum utilized in AgNPs synthesis on the inhibition of bacterial growth

Qaeed

Hendi

Obaid

et al. 2023

Sci Rep

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This study examined the effect of varying concentrations of Ocimum basilicum aqueous extract, which was done via the green synthesis of Silver nanoparticles (AgNPs), on the identification of the most effective concentration for bacteria inhibitory activity. Different concentrations of the aqueous Ocimum basilicum extract (0.25, 0.50, 0.75 and 1.00 mM) were used as reducing and stabilizing agent to synthesize AgNPs by means of the reduction method. The crystal structure and morphology of the NPs were characterized UV–Vis spectra, X-ray diffraction (XRD) and scanning electron microscopy (SEM). The antibacterial efficacy of AgNPs was studied against E. coli ATCC 35218 using well diffusion, MIC, MBC, and time-kill curve. The dark yellow color of the Ocimum basilicum aqueous solution indicates the successful synthesis process of the AgNPs. UV-spectra of the AgNPs display a gradual increase of absorption in sequence with concentration increase of aqueous Ocimum basilicum extract solution from 0.25 to 1.00 mM. This, in turn, led to a shift in the wavelength from 488 to 497 nm, along with a change in the nanoparticle size from 52 to 8 nm. The tests also showed a high activity of the particles against bacteria (E. coli), ranging between 15.6 and 62.5 µg/ml. Based on AgNPs, it was confirmed that an aqueous Ocimum basilicum extract can be used as an effective, reducing and stabilizing agent for the synthesis of different sizes of AgNPs based on the solvent concentration. The AgNPs also proved to be effective in inhibiting and killing bacteria.

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Section: Anti-bacterial Results Of Agnpssupporting

confidence: 94%

“…By using AgNPs with phage of ZCSE6, AgNPs were made via green synthesis using Ocimum Basilicum. Through this study, a synergistic activity was obtained against the growth of microbes, where the researcher found that the MIC and MBC values, which were 6.25 and 12.5 μg/ml, respectively, decreased to 1.5 μg/ml 38 .…”

mentioning

confidence: 88%

The effect of different aqueous solutions ratios of Ocimum basilicum utilized in AgNPs synthesis on the inhibition of bacterial growth

Qaeed

Hendi

Obaid

et al. 2023

Sci Rep

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“…In summary, silver microcompounds presented different characteristics due to the honey sample, method of biogenic synthesis used and application of a heat treatment. They presented similar Minimal Inhibitory Concentrations similar to smaller nanoparticles and much lower than concentrations found cytotoxic in our studies, as well as in literature [67][68][69][70][71][72][73][74][75][76][77][84][85][86][87][88][107][108][109][110][111][112] . Most microcompounds demonstrated themselves to be effective bactericidal agents at 125 µM.…”

Section: Discussionsupporting

confidence: 91%

Comparison of the Antibacterial Effect of Different Biological Silver Nanoparticles Synthetized and Integrated with Honeys

Clébis¹,

Scandorieiro²,

Spinosa

et al. 2022

nefm

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This study compares the morphologies, zeta potentials, and antibacterial effects a total 12 different microcompounds containing honey and silver nanoparticles, in a novel study of the difference between honey samples in nanoparticle synthesis, as well as the antibacterial interaction that those honey samples can have with the silver nanoparticles synthetized using them. Microcompounds were synthetized by combining silver nitrate solution with a honey sample and performing one of methods of biogenic synthesis: sunlight exposure, basification to pH 5 or basification to pH 10. Samples of each microcompound were also submitted to heat treatment, obtaining thus heated variants. Morphology and size data were obtained by Dynamic Light Scattering (DLS) analysis and Scanning Electron Microscopy (SEM); while zeta potential was measured by Electrophoretic Light Scattering. Broth microdilution, time-kill curves and SEM were used to access the antibacterial effect. Mean diameter of particles inside all microcompounds varied between 100 nm and 150 nm; and the zeta potential varied depending on the honey used. Minimal Inhibitory Concentrations (MIC) of microcompounds were between 15 μM and 500 μM. Time-kill curves showed that microcompounds had a faster and stronger effect against Escherichia coli than Staphylococcus aureus. Microcompounds obtained by basification to pH 5 or by sunlight were bactericidal, as they were capable of inhibiting bacterial growth (resulting in an antibacterial efficiency of 100% in 24 hours) at 125 μM against S. aureus and 62.5 μM against E. coli. SEM micrographs showed bacterial cells with lower cell density, blebs and other alteration after microcompound treatment.

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“…The MIC value for the AgNPs was determined as 3.12 µg mL -1 and 6.25 µg mL -1 for gram positive and negative bacteria, respectively [24]. The antibacterial effect of AgNPs which is formed by combining O. basilicum and Hibiscus sabdariffa L. plant extracts were investigated, and the MIC value was determined as 6.25 µg mL -1 [26].…”

Section: Resultsmentioning

confidence: 99%

Some biological potential of silver nanoparticles synthesized from Ocimum basilicum L.

Karakaş¹,

N²

2023

GSC Biol. Pharm. Sci.

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In our study, the ability to produce silver nanoparticles (AgNP) by green synthesis method using Ocimum basilicum L. plant was investigated. The color of the extract changed from yellow to brown, proving the presence of AgNPs. Scanning electron microscopy (SEM) and energy dispersed spectra (EDS) were used to characterized AgNPs. AgNPs ranged from 15 nm to 22 nm in size and were mostly spherical. The antimicrobial potential of AgNPs was tested against four bacterial and one yeast cultures by disc diffusion and minimum inhibitory concentration (MIC) methods. AgNPs significantly inhibited all test cultures. The highest antimicrobial and antibiofilm activities of plant extract and AgNP were found against Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 10231, respectively. In free radical scavenging activity (DPPH), the higher antioxidant activity were found 56.31±0.73% (plant extract) and 30.71±1.35% (AgNP). The higher 2,2'- Azino-bis (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS•+) cation radical scavenging acitivty were also determined 55.87±1.38% and 39.56±1%, respectively.

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Green Synthesis of Silver Nanoparticles Using Ocimum basilicum L. and Hibiscus sabdariffa L. Extracts and Their Antibacterial Activity in Combination with Phage ZCSE6 and Sensing Properties

Cited by 20 publications

References 66 publications

The effect of different aqueous solutions ratios of Ocimum basilicum utilized in AgNPs synthesis on the inhibition of bacterial growth

The effect of different aqueous solutions ratios of Ocimum basilicum utilized in AgNPs synthesis on the inhibition of bacterial growth

Comparison of the Antibacterial Effect of Different Biological Silver Nanoparticles Synthetized and Integrated with Honeys

Some biological potential of silver nanoparticles synthesized from Ocimum basilicum L.

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