Green synthesis of silver nanoparticles and their characterization by XRD

Mehta, B. K.; Chhajlani, Meenal; Shrivastava, B. D.

doi:10.1088/1742-6596/836/1/012050

Cited by 131 publications

(56 citation statements)

References 15 publications

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“…HR-TEM micrograph of biosynthesized silver nanoparticles and b particle size distribution XRD pattern of silver nanoparticles synthesized from mulberry leaf extract almost matches exactly with the reported standard value of silver which is 4.086 Å (JCPDS file no: 04-0783). Similar finding on biogenic synthesis of silver nanoparticles was earlier reported by Anandalakshmi et al[77] and Mehta et al[78].…”

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confidence: 89%

Biogenic synthesis of silver nanoparticles using S1 genotype of Morus alba leaf extract: characterization, antimicrobial and antioxidant potential assessment

2019

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Present study deals with synthesis and assessment of antimicrobial and antioxidant activity of silver nanoparticles using aqueous extract of mulberry leaves. The optical study showed the appearance of SPR peak in the range of 423-450 nm affirming nanosilver formation. FTIR analysis indicates the possible involvement of proteins, carbohydrate and secondary metabolites as reducing and capping agents. SEM, TEM and HR-TEM analysis reveals that the synthesized nanoparticles were spherical in shape with particle size ranges between 12 and 39 nm. EDX spectra showed maximum intensity at 3 keV, affirming silver crystal. XRD analysis showed silver nanoparticles were preferentially oriented along (111) revealing crystalline structure. DLS analysis confirms the stability of silver nanoparticles with zeta potential of + 37.4 mV. Silver nanoparticles showed effective antimicrobial activity against both gram positive and gram negative bacteria with highest activity against Salmonella typhimurium with MIC 40 µg/ml. Further silver nanoparticles also showed dose dependent antioxidant activity against free radicals like DPPH, ABTS + , superoxide and nitric oxide; besides this nanosilver also performed significant metal chelating activity.

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confidence: 89%

Biogenic synthesis of silver nanoparticles using S1 genotype of Morus alba leaf extract: characterization, antimicrobial and antioxidant potential assessment

2019

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“…This suggests that the synthesized AgNPs were in agreement with reference standard cubic Ag° of Joint Committee on Powder Diffraction Standards – JCPDS file no. 00‐001‐1167 (Mehta et al, ). Diffraction peak angles together with full width at half maximum height of the diffraction peaks for the synthesized nanoparticles are shown in Table .…”

Section: Resultsmentioning

confidence: 99%

One‐pot microwave‐assisted synthesis of size‐dependent l‐glutathione‐capped spherical silver nanoparticles suitable for materials with antibacterial properties

Nyamu

Ombaka

Masika

et al. 2019

J of Interdiscip Nanomed

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In the last years, there has been an alarming increase in antibiotic resistance by pathogenic microbes, which has become a major public health concern. There is a great interest in developing new antimicrobial for reducing the impact. Silver nanoparticles (AgNPs) as antibacterial agents are currently being studied to be used to fight these pathogenic microbes. The aim of the present study was to synthesize AgNPs of different sizes through the use of microwave and determine their antimicrobial activities. Synthesis of sizedependent L-glutathione-capped spherical nanoparticles through one-pot microwave synthesis was achieved, and their antimicrobial properties were determined. Different sizes of AgNPs between 5-10, 15-35, and 50-80 nm were made by varying the concentration of silver nitrate and using sodium borohydride (NaBH 4 ) as a reducing agent. L-glutathione was used to stabilize the AgNPs to prevent them from aggregation in the colloidal solution.The synthesized AgNPs showed ultraviolet absorption at around 400 nm with high concentration of AgNO 3 having sharp peaks. The formed particles were crystalline in nature with uniform spherical shape. The formed AgNPs were of crystalline size of 9. 94, 18.45, 34.96, 52.40, and 58.50 nm. Fourier transform infrared analysis confirmed conjugation of glutathione as a capping agent to AgNPs as the result of the formed spectra showing the absence of ─SH stretch. The high temperature generated by microwave helped to synthesize nanoparticles within a short time and by varying the concentration of AgNO 3 helped obtain the desired particle size. Glutathione conjugated well with AgNPs as a result of interaction of negative thiol resulting to colloidal stabilization and reduced aggregation. The antibacterial activity of AgNPs was found to be size dependent with the smaller size of 9.94 nm being more efficient than 18. 45, 34.96, 52.40, and 58.50 nm against the tested strains Bacillus subtilis (ATCC 6633), Escherichia coli (ATCC 25922), Salmonella spp. (ATCC 700623), and Staphylococcus aureus (ATCC 25923). Of the four stains, E. coli was found to be the least affected by all three different particle sizes of the synthesized AgNPs.

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“…The spectra were recorded by XRD system (Rigaku Corporation, Tokyo, Japan) by using Scherrer's formula: D= 0.9λ/βcosθ operating at 40 kV and a current of 30 mA with Cu Ka radiation. The diffracted pattern was scanned in 2θ ranges from 20° to 80° [19]. Dynamic light scattering measurements were carried out by using DLS (Zetasizer Nano ZSP, Malvern-Worce, UK).…”

Section: Characterization Of Biogenic Agnps By Using Uv-vis Xrd Dlsmentioning

confidence: 99%

Anti-pathogenic efficacy of biogenic silver nanoparticles through adherence and biofilm inhibition in multidrug resistant ESKAPE pathogens

Khan

Unnikrishnan

Ramalingam

2019

Preprint

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Background The current study aimed to produce AgNPs through a biogenic approach and assessed for their significant anti-pathogenic activities against multi-drug resistant ESKAPE pathogens. The biogenic AgNPs were synthesized through non-toxic manner and characterized by using UV-vis, XRD, DLS, TGA-DTA, FTIR, SEM along with EDX and UHRTEM were used to determine the absorption spectra, shape, size, thermal behaviour, functional groups, morphology, elemental constituents and defined particle size distribution profile, respectively. The AgNPs were evaluated for their anti-pathogenic effects against eleven strains of multi drug resistant ESKAPE pathogens by growth inhibition, biofilm adhesion, growth kinetics and Live/dead assays.Results The inhibitory range of AgNPs concentration was investigated as higher zones at escalating concentration (50 to 200 µg/ml). The growth kinetics of inhibition of all tested pathogens occurred after 4 hrs of treatment with AgNPs. Adherence assay exhibited highest inhibition in E. faecium (MCC 2763), P. aeruginosa (MTCC 1688) and E. species (MCC 2296) at 100µg/ml of AgNPs. The exposure of AgNPs increased the dead cell and consequently reduced cells density with AgNPs comparable with the effect of commercial antibiotics. The selected pathogens were found more sensitive to AgNPs than Cefotaxime/AgNO3 with the statistically significant (P < 0.05).Conclusion The emergence of drug resistance in ESKAPE pathogens are the extending reason for nosocomial infections, limiting the choice of antibiotics. Nanomaterials have been considered potential agents to prevent infections. Therefore, present study showed the broad spectrum potential and anti-pathogenic potency of biogenic AgNPs as an alternative to conventional antimicrobial agents.

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Green synthesis of silver nanoparticles and their characterization by XRD

Cited by 131 publications

References 15 publications

Biogenic synthesis of silver nanoparticles using S1 genotype of Morus alba leaf extract: characterization, antimicrobial and antioxidant potential assessment

Biogenic synthesis of silver nanoparticles using S1 genotype of Morus alba leaf extract: characterization, antimicrobial and antioxidant potential assessment

One‐pot microwave‐assisted synthesis of size‐dependent l‐glutathione‐capped spherical silver nanoparticles suitable for materials with antibacterial properties

Anti-pathogenic efficacy of biogenic silver nanoparticles through adherence and biofilm inhibition in multidrug resistant ESKAPE pathogens

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