Green Synthesis of Silver Nanoparticles using Salacca zalacca Extract as Reducing Agent and It’s Antibacterial Activity

Abstract

In this article, the synthesis of silver nanoparticles through a reduction reaction process using Salacca zalacca extract is reported. The AgNPs were characterized using X-ray diffraction, transmission electron microscopy, Fourier transform infrared and UV-visible spectrophotometry methods. The AgNPs antibacterial activity was determined against of Gram-positive bacteria (Staphylococcus epidermidis) and Gram-negative bacteria (Escherichia coli). The main functional groups contained in Salacca zalacca extract are carbonyl, hydroxyl and nitrile groups, which are believed to reduce the silver ions to metal. The surface plasmon resonance values of brownish red AgNPs are in the range of 410 nm to 460 nm. The structure of AgNPs is face centered cubic (FCC). The diameter of silver nanoparticles crystallite is 14.2 ± 2.6 nm. The AgNPs growth inhibition zones of Escherichia coli and Staphylococcus epidermidis are 9.6 mm and 9.2 mm, respectively.

Green Synthesis of Silver Nanoparticles with Snake Fruit Peel Extract: A Preliminary Study For Optimization of The Preparation Technique

“…Preparation of Snake fruit Peel Extract (SPE) AgNPs (Prodjosantoso et al, 2019) A total of 150 mL of the extract was added sequentially with AgNO3 solution (0.25 mM, 1.00 mM, 2.00 mM, and 4.00 mM) dropwise until the color turned more intense in a water bath at 30 0 C, 50 0 C, and 75 0 C and incubated for 15, 30, 60, 120, 240, and 1440 minutes before covering with aluminium foil.…”

“…This research followed the procedure reported by (Prodjosantoso et al, 2019) with modifications and AgNPs were synthesized using a volume ratio of 2:15 (20 mL) AgNO3 to 150 mL SPE as reducing and capping agents. In this context, the incubation procedure was carried out in a closed container in a dark room.…”

“…The solution incubated for 30 minutes at 30 o C with an AgNO3 concentration of 1.00 mM was measured using a UV-Vis Spectrophotometer in the wavelength range of 300 -700 nm. The nanoparticles were observed to determine the surface plasmon resonance (SPR) of AgNPs differentiated at a wavelength of 410 nm with an absorbance of 2.361 (Prodjosantoso et al, 2019). The stability of AgNPs at the incubation time can be seen from UV-Vis absorption peak in the range of 350 nm -450 nm under the characteristics of SPR.…”

“…Recently, biological methods have been selected as an alternative in the synthesis of nanoparticles using plants and microorganisms (Abdel-Halim et al, 2011;Iravani et al, 2014;Makarov et al, 2014). Some examples of plants reported in the synthesis are galangal (Rizkita et al, 2023), sambiloto (Purnomo et al, 2017), and snake fruit (Prodjosantoso et al, 2019).…”

Analisis Bibliometrik Penelitian Bio-Sintesis Nanopartikel Perak (NP-Ag) di Indonesia

“…Klaster 3 ditunjukkan dengan simpul berwarna biru (Gambar 5) dengan kata kunci terdiri dari "e. coli", "nanoparticlesa", dan "silver". Klaster ini fokus pada uji aktifitas antibakateri NP-Ag yang disintesis menggunakan agen bio-reduksi yang diperoleh dari tumbuhan Indonesia diantaranya Impatiens balsamina dan lantana camara [21], Salacca zalacca [22], Diospyros discolor Willd [23], dan Scaevola frutescen (Mill.) Krause [24].…”