Green Synthesis of Silver Nanoparticle and Its Antibacterial Activity

Abstract

Silver nanoparticle (Ag-np) was successfully synthesized by using the simple green chemistry method. Its antibacterial activity was studied against Staphylococcus aureus and Escherichia coli according to agar well diffusion method. The green synthesis was carried out by mixing the silver nitrate, glucose, and starch as precursors at 90 0 C for 4 hours. The UV-Vis spectra show the characteristic of spherical Ag-np in the range of 410-425 nm and it is confirmed by TEM image. The Ag-np has an average diameter of 8.41 ± 4.37 nm. As the reductant mole composition increases, the SPR decreases and shifts to red due to the further growth of the Ag-np. When the stabilizer composition increases, the SPR intensity also increases and shifts to red. The formed Ag-np is stable even after one-month synthesis. The antibacterial test on the Ag-np was carried out against gram-positive bacteria (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli). The result shows that a higher concentration of Ag-np is required to kill Staphylococcus aureus compared to Escherichia coli.

Characterization and Effect of Ag(0) vs. Ag(I) Species and Their Localized Plasmon Resonance on Photochemically Inactive TiO2

“…The deposited Ag species contributed to an increase in the material's absorption towards the visible region as seen in the 420-700 nm wavelength range (see inset graph). This is in accordance with previous work on visible light absorption in Ag nanoparticles species at 410-425 nm due to the surface plasmon resonance effect [40][41][42][43][44][45][46] (Figure 2b). The absorption, with a maximum near 425 nm, can be attributed to the plasmon absorption of spherical Ag nanoparticles [45], whereas the broad absorption reaching down to~700 nm may be attributed to differently shaped Ag nanoparticles, such as cube-like structures [45], or to the absorption of Ag 2 O [47].…”

Green synthesis of silver nanoparticles mediated by traditionally used medicinal plants in Sudan

“…Stability of the synthesized silver nanoparticles colloid at pH 4 was also reported [19]. Similarly, Krishnan et al described the stability of silver nanoparticles under different pH (2)(3)(4)(5)(6)(7)(8)(9) and found no effect on the nanoparticles morphology and their SPR peak [28]. Interestingly, Sahni et al have reported formation of monodisperse silver nanoparticles by addition of ammonia [37].…”

“…Recently, plant-mediated green synthesis of silver nanoparticles is developing into a new and important branch of nanotechnology. It has emerged and gained importance because it is eco environmental and effective cost, with lesser toxicity when related to chemical hazards [4]. Among physical and chemical nanoparticles synthesis, green synthesis has several advantages.…”

Synthesis, Characterization, Surface Functionalization of Magnetite Nanoparticles and Their Antibacterial Studies

“…[1][2][3] Nanoparticles may be synthesized using various cost-effective and eco-friendly methods such as sol-gel method, green synthesis and nanoprecipitation technique. [4][5][6][7] Surface functionalized magnetite nanoparticles have become more popular among researchers due to their vast applications. The surface functionalization of magnetite nanoparticles with protein enhances the capabilities of these nanoparticles.…”