Green Synthesis of Silver Nanocomposites of Nigella sativa Seeds Extract for Hepatocellular Carcinoma

Abstract

Background: Silver nanoparticles play a significant role in bioavailability and refining the compatibility of natural drugs in the treatment of various chronic diseases including different types of cancer. Objective: Green synthesis of silver nanocomposites of Nigella sativa seeds extract to evaluate the anticancer effects against hepatocellular carcinoma using HepG2 cell lines. Methods: The AgNCs were developed by treating aqueous extract of N. sativa seeds treated with silver nitrate (1mM) solution and were used to test its efficacy against hepatocellular carcinoma using HepG2 cell lines. Results and Discussion: The Surface Plasmon Resonance (SPR) of prepared AgNCs showed a peak at 432 nm via UV spectroscopy. The selected N. sativa AgNCs were characterized for polydispersity, surface charge and size and the results showed 0.215±0.093 polydispersity index (PDI), zeta potential 18.8±0.372 mV and size range 10-20 nm, respectively. The Fourier transform infrared spectroscopy (FTIR) also showed various peak of functional groups that are possibly involved in the reduction of silver ion and synthesized the N. sativa silver nanocomposites, respectively. N. sativa AgNCs showed 89.954% drug release while in the case of extract release, it was only 33.821% in 24 hrs. Further, in vitro studies of N. sativa AgNCs against hepatocellular carcinoma showed good cytotoxic effect p<0.05 with 7.16 µg/ml IC50 value. Conclusion: Thus, the present results revealed that green synthesis of N. sativa AgNCs can be an alternative tool for clinical application in cancer therapy; however, there is a need to find the mechanism and role of AgNCs inside the individual.

Green synthesis of silver nanoparticles mediated by traditionally used medicinal plants in Sudan

“…Biosynthesized silver nanoparticles and Piper nigrum extract showed promising anticancer activity against breast cancer cells (MCF-7) and human pharynx cancer cell line (Hep-2) [28]. Other findings revealed that silver nanoparticles from Nigella sativa seeds were found to be effective against hepatocellular carcinoma using HepG2 cell lines [40].…”

“…UV-visible spectrophotometry is a readily available technique allows fast identification and characterization of silver nanoparticles. It gives strong absorbance band known as surface plasmon resonance (SPR) in the range 400-500 nm due to the interaction between light and mobile surface electrons of silver nanoparticles [16,39,40].…”

Green synthesized plant-based silver nanoparticles: therapeutic prospective for anticancer and antiviral activity

“…Under the influence of the electric field, the velocity of the particles is evaluated at which they travel towards the electrodes [20]. AgNPs synthesized using seed extract of Nigella sativa and leaf extracts of Gloriosa superba and Cynara scolymus showed that particles possess negative charge with the potential of − 18.8 ± 0.372, − 27.0, and − 32.3 ± 0.8 mV respectively [50][51][52]. Zeta potential of the Phyla dulcis extract loaded silver nanoparticles was analyzed and values were between − 20 and − 24 mV indicated that the AgNPs are relatively stable [53].…”

Potential activities of nanoparticles synthesized from Nigella sativa L. and its phytoconstituents: An overview

“…Almatroudi et al (2020) also supported the former study and suggested that Ns-AgNPs showed significant cytotoxic ability and substantially killed human breast cancer cell (HCC-712) viability. In an in vitro test carried out using Ns-AgNPs on hepatocellular carcinoma, it has been observed that these nanoparticles possess a cytotoxic effect of p<0.05 with a 7.16 µg/ml IC 50 value (Usmani et al, 2019). It has been observed that Ns-AgNP at an amount of 12.5 µg/ml showed antibiofilm activity by 88.42% for Enterococcus faecalis, 84.92% for E. coli, 81.86% for Klebsiella pneumonia, 82.84% for Staphylococcus aureus, and 49.9% for Pseudomonas aeruginosa (Almatroudi et al, 2020).…”