Green synthesis of pyrazolines as an anti-inflammatory, antimicrobial and antioxidant agents

Abstract

Green methods such as microwave technique and grinding technique has been found superior over the conventional method in view of reaction time and energy requirement. Comp research (2a-i), (3a-i) have been characterized by 1H NMR, 13C NMR, IR and mass spectral analyses. They were screened for their in vitro anti-inflammatory, antimicrobial and in vitro antioxidant activities. Antimicrobial activity was performed by Agar plate method and comp. 2d & 2e a active against gram negative bacteria E. coli and S. aureus, the comp. 3c and 3g show antioxidant activity as (IC50 = 48.13 and 46.03µg/ml), Comp’ 3c shows respectively anti-inflammatory activity at (EC50 = 11.00 ± 1.26 µg/mL). Comp. ,3c ,3d and 3e showed good anti-inflammatory activity at concentration (EC50 = 11.70 ± 0.98 µg/mL) & 11.70 ± 0.98 µg/mL respectively.