Green synthesis of pyrazolines as anti-inflammatory, antimicrobial and antioxidant agents

Abstract

Green methods such as microwave technique and grinding technique have been found superior over the conventional method in view of reaction time and energy requirement. Pyrazoline derivatives , 2a-i and 3a-i, were synthesized both conventional heating and by microwave irradiation, the synthesized compounds were characterized by 1H NMR, 13C NMR, IR and mass spectral analyses. They were screened for their in vitro anti-inflammatory, antimicrobial and antioxidant activities. Antimicrobial activity was performed by Agar plate method and 2d & 2e were active against Gram negative bacteria E. coli and S. aureus, 3c and 3g showed antioxidant activity (IC50=48.13 and 46.03μg/ml),3c shows respectively anti-inflammatory activity at (EC50= 11.00±1.26 µg/mL) 3c ,3d and 3e showed good anti-inflammatory activity at concentration (EC50= 11.70±0.98 µg/mL) & 11.70±0.98 µg/mL respectively.