Green Synthesis of Iron Nanoparticles for Investigation of Biofilm Inhibition Property

Abstract

Green metal nanoparticles (NPs) have emerged as efficient biofilm inhibitors. Citrus macroptera (CM) fruit extract mediated iron (Fe) NPs, i. e., CM-FeNPs were thus synthesized for studying biofilm inhibition property. The shape (spherical), size (12 nm), and crystallinity (α-Fe 0 bcc) of the CM-FeNPs were confirmed by studying mainly Transmission Electron microscopy (TEM) and X-ray Diffractometer (X-RD) tools. The particles inhibit biofilm growth of a bacteria Pseudomonas aeruginosa (P. aeruginosa) by inhibiting the functions of extracellular polymeric substances (EPS) and virulence factors to ∼ 80 %. Scanning Electron microscopy (SEM) images of the bacteria in presence of the CM-FeNPs (4600 nM) in combination with azithromycin (AZI) evidence the inhibition of biofilm growth > 80 %. Molecular docking studies support experimental findings and result significant negative binding energies (e. g., isoplatydesmine/LasR: À 8.25 kcal/mole, isoplatydesmine/MvfR: À 7.56 kcal/mole) from bioactive molecules/biofilm proteins interactions. Thus, CM-FeNPs could be considered as potential biofilm inhibitors, and as an alternative to antibiotic drugs.

Effect of Camellia sinensis Origin and Heat Treatment in the Iron Oxides Nanomaterials Composition and Fenton Degradation of Methyl Orange

“…The powder XRD patterns of the as-prepared BTn Fe materials showed a large peak around 23° (Figure 1a), typical of amorphous IONPs coated with the polyphenols. [6][7][8][9][10] SEM images (Figure S1, SI section) showed particles of undefined and irregular shape, with a wide size range due to agglomeration. The corresponding EDS spectra of the sample BT1 Fe in Figure 1b showed carbon and oxygen as the main elements around 60 and 35 at%, respectively.…”

“…[1][2][3] Different plants as black tea, Yerba Mate, grape seed, green tea, oolong tea, eucalyptus leaf, have produced different iron nanomaterials because of the different polyphenol composition and synthesis condition, which consequently affects the IONPs properties. [6][7][8][9][10][11][12][13][14][15] As reported in the literature, [6][7][8][9][10] most of the green IONPs are amorphous materials due to the high content of polyphenols, which precludes formation of defined crystalline nanomaterials as well as formation of iron oxides of scientific and technological interest. Thermal treatment has been described for green IONPs to favor the formation of crystalline and well-defined iron oxides.…”

Reinforcement and Antibacterial Properties of Hand Embroidery Threads Based on Green Nanocoatings

“…They also play a role in fostering social progress and creating job opportunities. Interestingly, the features of the textiles and threads used, such as their breaking strength, antibacterial properties, and UV protection from harmful rays, have had an impact on the handcraft industries in numerous countries [1][2][3][4][5][6]. Hand embroidery is one of the best-known crafts; however, the quantity of threads used determines how much of the design is used in ambiguous areas.…”

N-Doped Carbon Nanoparticles as Antibacterial Agents on Escherichia coli: The Role of the Size and Chemical Composition of Nanoparticles

“…Therefore, the design of new biocompatible materials as substitutes of antibiotics is still a challenge. Some metals such as Ag, Au and Pt [10,[12][13][14][15], as well as metal oxides (CuO, Fe 2 O 3 , ZnO) [16][17][18][19] or hybrid compounds formed by metal oxides doped with a second metal (Mo/NiO) [20] or even nanoparticles made up of three metal oxides (CuO/ZnO/MnO 2 ) [21] present high antibacterial activity and have been successfully used as a coating in the prevention of biofouling [14,15,22,23] and in cancer therapy treatments [14]. The main drawback of these nanoparticles is their moderate chemical stability, which can lead to high toxicity due to the release of metal ions [13,24,25].…”