Green synthesis of gold nanoparticles (AuNPs) using <i>Caulerpa racemosa</i> and evaluation of its antibacterial and cytotoxic activity against human lung cancer cell line

Pitchai, Palaniappan; Subramani, Prabhu; Selvarajan, Ramganesh; Sankar, Raman; Ravikumar, Vasulinga T.; Sibanda, Timothy

doi:10.1080/25765299.2022.2127510

Cited by 11 publications

(4 citation statements)

References 46 publications

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“…The peak at 1387 cm −1 is corresponding to aromatic amine group (C–N) stretch bond 56 . The peak at 1072 cm −1 was indicative of amines 57 . The peak at 867.56 cm −1 correlates to C–H stretch bond which is indicative of alkenes' functional group 58 .…”

Section: Resultsmentioning

confidence: 99%

Process optimization for gold nanoparticles biosynthesis by Streptomyces albogriseolus using artificial neural network, characterization and antitumor activities

El-Naggar,

El-Sawah,

Elmansy

et al. 2024

Sci Rep

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Gold nanoparticles (GNPs) are highly promising in cancer therapy, wound healing, drug delivery, biosensing, and biomedical imaging. Furthermore, GNPs have anti-inflammatory, anti-angiogenic, antioxidants, anti-proliferative and anti-diabetic effects. The present study presents an eco-friendly approach for GNPs biosynthesis using the cell-free supernatant of Streptomyces albogriseolus as a reducing and stabilizing agent. The biosynthesized GNPs have a maximum absorption peak at 540 nm. The TEM images showed that GNPs ranged in size from 5.42 to 13.34 nm and had a spherical shape. GNPs have a negatively charged surface with a Zeta potential of − 24.8 mV. FTIR analysis identified several functional groups including C–H, –OH, C–N, amines and amide groups. The crystalline structure of GNPs was verified by X-ray diffraction and the well-defined and distinct diffraction rings observed by the selected area electron diffraction analysis. To optimize the biosynthesis of GNPs using the cell-free supernatant of S. albogriseolus, 30 experimental runs were conducted using central composite design (CCD). The artificial neural network (ANN) was employed to analyze, validate, and predict GNPs biosynthesis compared to CCD. The maximum experimental yield of GNPs (778.74 μg/mL) was obtained with a cell-free supernatant concentration of 70%, a HAuCl4 concentration of 800 μg/mL, an initial pH of 7, and a 96-h incubation time. The theoretically predicted yields of GNPs by CCD and ANN were 809.89 and 777.32 μg/mL, respectively, which indicates that ANN has stronger prediction potential compared to the CCD. The anticancer activity of GNPs was compared to that of doxorubicin (Dox) in vitro against the HeP-G2 human cancer cell line. The IC50 values of Dox and GNPs-based treatments were 7.26 ± 0.4 and 22.13 ± 1.3 µg/mL, respectively. Interestingly, treatments combining Dox and GNPs together showed an IC50 value of 3.52 ± 0.1 µg/mL, indicating that they targeted cancer cells more efficiently.

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Section: Resultsmentioning

confidence: 99%

Process optimization for gold nanoparticles biosynthesis by Streptomyces albogriseolus using artificial neural network, characterization and antitumor activities

El-Naggar,

El-Sawah,

Elmansy

et al. 2024

Sci Rep

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“…A typical disc diffusion technique was utilized for antibacterial experiments with gram-positive Staphylococcus aureus and gram-negative Escherichia coli [ 66 ]. Sterile paper discs measuring 6 mm in diameter were placed on swabbed MHA plates.…”

Section: Methodsmentioning

confidence: 99%

Electrochemical investigations and antimicrobial activity of Au nanoparticles photodeposited on titania nanoparticles

Al-Maydama,

Jamil,

Awad

et al. 2024

Heliyon

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“…The algal extracts act as stabilizing agents, preventing the agglomeration and growth of nanoparticles. The green synthesis of ZnO-NPs using algal extracts is a relatively simple and low-cost process that requires minimal technical expertise and equipment [ 33 ]. The green synthesis of ZnO-NPs using algal extracts has numerous industrial applications, including catalysis, solar cells, paints, UV light-emitting devices, electrical devices, biomedicine, and cosmetics [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Algal Extracts for Green Synthesis of Zinc Oxide Nanoparticles: Promising Approach for Algae Bioremediation

et al. 2023

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Zinc oxide nanoparticles (ZnO-NPs) possess unique properties, making them a popular material across various industries. However, traditional methods of synthesizing ZnO-NPs are associated with environmental and health risks due to the use of harmful chemicals. As a result, the development of eco-friendly manufacturing practices, such as green-synthesis methodologies, has gained momentum. Green synthesis of ZnO-NPs using biological substrates offers several advantages over conventional approaches, such as cost-effectiveness, simplicity of scaling up, and reduced environmental impact. While both dried dead and living biomasses can be used for synthesis, the extracellular mode is more commonly employed. Although several biological substrates have been successfully utilized for the green production of ZnO-NPs, large-scale production remains challenging due to the complexity of biological extracts. In addition, ZnO-NPs have significant potential for photocatalysis and adsorption in the remediation of industrial effluents. The ease of use, efficacy, quick oxidation, cost-effectiveness, and reduced synthesis of harmful byproducts make them a promising tool in this field. This review aims to describe the different biological substrate sources and technologies used in the green synthesis of ZnO-NPs and their impact on properties. Traditional synthesis methods using harmful chemicals limit their clinical field of use. However, the emergence of algae as a promising substrate for creating safe, biocompatible, non-toxic, economic, and ecological synthesis techniques is gaining momentum. Future research is required to explore the potential of other algae species for biogenic synthesis. Moreover, this review focuses on how green synthesis of ZnO-NPs using biological substrates offers a viable alternative to traditional methods. Moreover, the use of these nanoparticles for industrial-effluent remediation is a promising field for future research.

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Green synthesis of gold nanoparticles (AuNPs) using Caulerpa racemosa and evaluation of its antibacterial and cytotoxic activity against human lung cancer cell line

Cited by 11 publications

References 46 publications

Process optimization for gold nanoparticles biosynthesis by Streptomyces albogriseolus using artificial neural network, characterization and antitumor activities

Process optimization for gold nanoparticles biosynthesis by Streptomyces albogriseolus using artificial neural network, characterization and antitumor activities

Electrochemical investigations and antimicrobial activity of Au nanoparticles photodeposited on titania nanoparticles

Algal Extracts for Green Synthesis of Zinc Oxide Nanoparticles: Promising Approach for Algae Bioremediation

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