Green synthesis of chlorella-derived carbon dots and their fluorescence imaging in zebrafish

Wang, Yue; Gu, Zhihi; Dong, Jingyi; Zhu, Jie; Liu, Cunguang; Li, Guohan; Lu, Meichen; Han, Jian; Cao, Shengnan; Chen, Liyong; Wang, Wei

doi:10.1039/d3ra07623g

Cited by 1 publication

(1 citation statement)

References 34 publications

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“…Figures 6a−6c clearly depict the fluorescence absorption of the CA, AA, and CA+AA CDs during the embryonic and larval stages of zebrafish, spanning from 6 to 72 h of exposure. The imaging outcomes of the present study are consistent with the findings of Wang and others in 2024, 43 who observed that zebrafish exposed to CD solutions at concentrations of 0.5 mg/mL during 24 to 96 hpf demonstrated remarkable fluorescence uptake toward green synthesis of chlorella-derived CDs. A similar uptake pattern was observed by Liu and team members 44 using a hydrothermal synthesis approach for CDs in zebrafish at the 6 hpf and 5 dpf stages exposed to a 5 mg/mL concentration for a 1 h incubation period.…”

Section: Optical Properties Of Fluorescent Cdssupporting

confidence: 92%

Single-Step Low-Temperature Synthesis of Carbon Dots for Advanced Multiparametric Bioimaging Probe Applications

Ghosh,

Gautam,

Gupta

et al. 2024

ACS Appl. Bio Mater.

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Carbon dots (CDs) have recently emerged in biomedical and agricultural domains, mainly for their probe applications in developing efficient sensors. However, the existing high-temperature approaches limit the industrial level scaling up to further translate them into different products by mass-scale fabrication of CDs. To address this, we have attempted to lower the synthesis temperature to 140 °C and synthesized different CDs using different organic acids and their combinations in a one-step approach (quantum yield 3.6% to 16.5%; average size 3 to 5 nm). Further, sensing applications of CDs have been explored in three different biological models, mainly Danio rerio (zebrafish) embryos, bacterial strains, and the Lactuca sativa (lettuce) plant. The 72 h exposure of D. rerio embryos to 0.5 and 1 mg/mL concentrations of CDs exhibited significant uptake without mortality, a 100% hatching rate, and nonsignificant alterations in heart rate. Bacterial bioimaging experiments revealed CD compatibility with Gram-positive (Bacillus subtilis) and Gram-negative (Serratia marcescens) strains without bactericidal effects. Furthermore, CDs demonstrated effective conduction and fluorescence within the vascular system of lettuce plants, indicating their potential as in vivo probes for plant tissues. The single-step low-temperature CD synthesis approach with efficient structural and optical properties enables the process as industrially viable to up-scale the technology readiness level. The bioimaging of CDs in different biological models indicates the possibility of developing a CD probe for diverse biosensing roles in diseases, metabolism, microbial contamination sensing, and more.

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Section: Optical Properties Of Fluorescent Cdssupporting

confidence: 92%

Single-Step Low-Temperature Synthesis of Carbon Dots for Advanced Multiparametric Bioimaging Probe Applications

Ghosh,

Gautam,

Gupta

et al. 2024

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

Green synthesis of chlorella-derived carbon dots and their fluorescence imaging in zebrafish

Abstract: Schematic illustration of fluorescence imaging and toxicology study of chlorella-derived CDs in zebrafish.

Cited by 1 publication

References 34 publications

Single-Step Low-Temperature Synthesis of Carbon Dots for Advanced Multiparametric Bioimaging Probe Applications

Single-Step Low-Temperature Synthesis of Carbon Dots for Advanced Multiparametric Bioimaging Probe Applications

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