Green synthesis of chitosan capped silver nanoparticles and their antimicrobial activity

Abstract

Chitosan is a polymeric compound with functional groups which enable surface binding to nanoparticles and antibacterial activity. The antimicrobial activity was studied using silver nanoparticles with varied concentrations of chitosan. The nanoparticles were synthesized through a simple and environmentally friendly method at room temperature. Spherical particles with average sizes between 2 and 6 nm were obtained and their crystallinity showed a face-centered cubic phase. The evidence of chitosan presence on the nanoparticle surface was confirmed by the characteristic diffraction peak of chitosan and by FTIR spectra where the bonding of amine group could be depicted. The chitosan-capped silver nanoparticles showed good antibacterial and antifungal activities with MIC values between 0.20 and 1.5 mg.mL-1 compared to those obtained from most of references (up to 6.25 mg.mL-1) on the selected gram-positive (Staphylococcus aureus, Enterococcus faecalis), gram-negative (Klebsiella pneumoniae, Pseudomonas aeruginosa ) bacteria and fungi (Candida albicans, Cryptococcus neoformans).

Nanosystems for the Encapsulation of Natural Products: The Case of Chitosan Biopolymer as a Matrix

“…Metal nanoparticles have been also coated with chitosan biopolymer developing new hybrid coated nanomaterials which present improved biological and physicochemical properties [ 213 ]. Hybrid chitosaninorganic nanoparticles combine materials with different mechanisms of action and have been innovatively developed as improved antibacterial [ 222 ], antimicrobial [ 223 ], anticancer [ 224 ] and wound healing agents [ 225 ].…”

“…The results demonstrated increased cytotoxicity of the coated NPs while the presumable mechanism of killing effect was the intracellular ROS generation [ 228 ]. Moreover, the differences in the cell morphology are indicative of the cell death via apoptosis [ 223 ].…”

Efficacy of chitosan silver nanoparticles from shrimp-shell wastes against major mosquito vectors of public health importance

“…Further, the modified chitosan nanoparticles were used for cancer therapy [23]. Cs-AgNPs are of interest because of their unique properties that can be incorporated into antifungal and antimicrobial applications [24,25]. Very recently, Alebouyeh et al [26] noticed that Penaeus semisulcatus-extracted chitosan nanoparticles highly inhibit the growth of Listeria monocytogenes and Salmonella typhi.…”

New Perspectives of Using Chitosan, Silver, and Chitosan–Silver Nanoparticles against Multidrug‐Resistant Bacteria

“…[ 117 ] The most common, simple, and effective approach is based on the chemical reduction of Ag + ions to metal Ag 0 by organic and inorganic reducing agents. [ 118 ] One of the most applied methods to synthesize AgNPs is proposed by Lee and Meisel [ 119 ] based on studies of Turkevich et al. [ 120 ] These authors standardized the chemical synthesis by reducing the Ag + ions with sodium citrate or sodium borohydride (NaBH 4 ) in an aqueous solution to yield a colloidal and stable silver dispersion.…”

“…[ 32 ] Chitosan is considered a suitable capping agent due to the presence of two reactive functional groups (NH 2 and OH) with a strong affinity to metal ions that facilitate the interaction of chitosan to metals. [ 118,172 ] In Cs‐AgNPs, the metal AgNPs chelate the lone pair electrons provided by oxygen and nitrogen atoms of chitosan to disperse the AgNPs in CsNPs. [ 164 ] Alternatively, a chitosan solution can be added to an AgNO 3 solution when producing AgNPs.…”