Green synthesis of assembled silver nanoparticles in nano capsules of <i>Peganum harmala</i> L leaf extract. Antibacterial activity and conjugate investigation

Alomari, Abdulaziz Ali; Fares, Kout El Kloub; Moustafa, Nagy E.

doi:10.1080/23312009.2018.1532374

Cited by 17 publications

(12 citation statements)

References 26 publications

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“…Leaf‐extracted polyol macromolecules can be associated with AuNPs in templates, capsules, or cavities formed by inter‐ and intra‐hydrogen bonds, where they gather, grow, and condense AuNPs . As shown previously, AgNPs were sustainably hosted in noncapsules formed by higher molecular weight polyols extracted from the leaf extract . In comparison, AuNPs were temporarily hosted in nanocapsules, the stretched nucleation mechanism suited to the regular shape and size of AuNPs.…”

Section: Resultsmentioning

confidence: 54%

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Green synthesis and bactericidal activities of isotropic and anisotropic spherical gold nanoparticles produced using Peganum harmala L leaf and seed extracts

Moustafa

Alomari

2019

Biotech and App Biochem

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Shape, size, and homogeneity affect the biological activity of gold nanoparticles (AuNPs) in nanomedicine and catalytic applications. Here we biosynthesized monodispersed isotropic and polydispersed anisotropic spherical AuNPs from leaf and seed extract broths of the medicinal plant Peganum harmala L. (Ph. L). Synthesized AuNPs were characterized by ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy (FT-IRS), field-emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The antimicrobial activity of AuNPs against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) human pathogens was also assessed. Leaf-and seed-derived AuNPs had characteristic localized surface plasmon resonances of 530 and 578 nm, respectively. TEM, FE-SEM, EDX, and XRD revealed the formation of elemental face-centered cubic spherical monodispersed isotropic AuNPs of average size 43.44 nm and polydispersed anisotropic AuNPs of average size 52.04 nm from leaf and seed extract broths, respectively. FT-IR revealed polyphenols and alcohols as responsible for AuNP capping, reduction, and protection. Anisotropic AuNPs showed no antibacterial activity, whereas isotropic AuNPs showed good inhibition of both E. coli and S. aureus. This represents a simple and ecofriendly protocol for the green synthesis of monodispersed isotropic spherical AuNPs, which may have value in a variety of applications.

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Section: Resultsmentioning

confidence: 54%

“…Consequently, both extracts produce different biomolecular conformations or/and formulations in aqueous media with diverse shapes and morphologies of the synthesized nanoparticles. Both leaf and seed AgNPs have shown different antibacterial activities .…”

Section: Introductionmentioning

confidence: 99%

Green synthesis and bactericidal activities of isotropic and anisotropic spherical gold nanoparticles produced using Peganum harmala L leaf and seed extracts

Moustafa

Alomari

2019

Biotech and App Biochem

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“…Extract of Oscillatoria limnetica conjugated with silver nanoparticles exhibited strong anti-bacterial activity against multidrug-resistant bacteria as well as cytotoxic effects against both human breast cell line and human colon cancer cell line [51]. Synthesis of silver chloride nanoparticles (AgCl-NPs), using walnut green husk extract as well as silver nanoparticles with Peganum harmala L leaf extract resulted in signi cant inhibitory effects against Escherichia coli and S.aureus clinical isolates [52], [53]. Bio-nanotechnology has been not studied on protozoan species extensively.…”

Section: Discussionmentioning

confidence: 99%

Tannic acid-modified silver nanoparticles enhance anti-Acanthamoeba activity without increasing cytotoxicity of the contact lens solutions

Hendiger

Padzik

Żochowska

et al. 2020

Preprint

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Background: Free living amoebae of Acanthamoeba genus are cosmopolitan, widely distributed protozoans causing severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). Majority of the increasing number of AK cases are associated with contact lenses use. Due to lack of effective therapies against AK, proper eye hygiene and effective contact lenses disinfection are crucial in prevention of this infection. Currently available multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent Acanthamoeba keratitis infections. Synthesized nanoparticles have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that plant metabolites, including tannins, present anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of the tannic acid-modified silver nanoparticles (AgTANPs) conjugated with selected multipurpose contact lens solutions. Methods: The anti-amoebic activity of pure contact lens care solutions and nanoparticles conjugated with contact lens care solutions were examined in vitro by colorimetric assay, based on the oxido-reduction of AlamarBlue. The cytotoxicity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. Results were statistically analyzed by ANOVA and Student-Newman-Keuls tests using the p<0.05 level of statistical significance. Results: The obtained results showed that nanoparticles enhanced anti-Acanthamoeba activity of the tested contact lens solutions without increasing their cytotoxicity profile. The activity is enhanced within minimal disinfection time recommended by the manufacturer. Conclusions: The conjugation of selected contact lens solutions with AgTANPs might be a novel and promising approach as part of preventive actions of Acanthamoeba keratitis infections among contact lens users.

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“…Extract of Oscillatoria limnetica conjugated with silver nanoparticles exhibited strong anti-bacterial activity against multidrug-resistant bacteria as well as cytotoxic effects against both human breast cancer cell line and human colon cancer cell line [53]. Synthesis of silver chloride nanoparticles (AgCl-NPs), using walnut green husk extract as well as silver nanoparticles with Peganum harmala L. leaf extract resulted in signi cant inhibitory effects against Escherichia coli and S. aureus clinical isolates [54,55]. Bio-nanotechnology has been not studied on protozoan species extensively.…”

Section: Discussionmentioning

confidence: 99%

Tannic acid-modified silver nanoparticles enhance anti-Acanthamoeba activity without increasing cytotoxicity of three multipurpose contact lens solutions

Hendiger

Padzik

Żochowska

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Free living amoebae of the genus Acanthamoeba are cosmopolitan, widely distributed protozoans causing a severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). The majority of the increasing number of AK cases are associated with contact lenses use. Appropriate eye hygiene and effective contact lenses disinfection are crucial in prevention of this infection because of the lack of effective therapies against AK. Currently available multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent Acanthamoeba keratitis infections. Synthesized nanoparticles have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that plant metabolites, including tannins, present anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of the tannic acid-modified silver nanoparticles (AgTANPs) conjugated with the selected multipurpose contact lens solutions.Methods: The anti-amoebic activity of pure contact lens care solutions and nanoparticles conjugated with contact lens care solutions were examined in vitro by a colorimetric assay, based on the oxido-reduction of AlamarBlue. The cytotoxicity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. The results were statistically analyzed by ANOVA and Student-Newman-Keuls tests using the P < 0.05 level of a statistical significance.Results: We show that nanoparticles enhanced anti-Acanthamoeba activity of the tested contact lens solutions without increasing their cytotoxicity profile. The activity is enhanced within the minimal disinfection time recommended by the manufacturer.Conclusions: The conjugation of the selected contact lens solutions with AgTANPs might be a novel and promising approach as a part of preventive actions of Acanthamoeba keratitis infections among contact lens users.

show abstract

Green synthesis of assembled silver nanoparticles in nano capsules of Peganum harmala L leaf extract. Antibacterial activity and conjugate investigation

Cited by 17 publications

References 26 publications

Green synthesis and bactericidal activities of isotropic and anisotropic spherical gold nanoparticles produced using Peganum harmala L leaf and seed extracts

Green synthesis and bactericidal activities of isotropic and anisotropic spherical gold nanoparticles produced using Peganum harmala L leaf and seed extracts

Tannic acid-modified silver nanoparticles enhance anti-Acanthamoeba activity without increasing cytotoxicity of the contact lens solutions

Tannic acid-modified silver nanoparticles enhance anti-Acanthamoeba activity without increasing cytotoxicity of three multipurpose contact lens solutions

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