Green Synthesis of Anti-bacterial Nano Silver by Polysaccharide from Bletilla Striata

Abstract

The silver nanoparticle is a good antibacterial material being used as a broad-spectrum fungicide, including against some multidrug-resistant strains. Compared with the normal chemical and physical preparation methods, green synthesis has attracted wide attention, because of the pharmaceutical activities of the natural product, mild reaction conditions, and environmentally friendly, etc. In this study, the synthesis of silver nanoparticles (Ag NPs) was prepared from Bletilla striata polysaccharide (BSP) and characterized by UV-vis spectroscopy and Dynamic Light Scattering (DLS). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) indicated the morphology of Ag NPs was subspherical with an average size of 20–35 nm. Bletilla striata polysaccharide not only can be used as a natural reducing agent, but also has good repairing ability. Moreover, the antibacterial experimental results showed its great antimicrobial activity against Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Escherichia coli) and Candida albicans.

Polysaccharides from Russula: a review on extraction, purification, and bioactivities

“…As a result, attempts have been made to extract novel natural products as anti-bacterial agents, heralding a new frontier in this field of research. Abundant natural sources of anti-bacterial agents present a treasure trove for medicinal exploration ( 93 ). The naturally occurring anti-bacterial drugs used either directly as whole plant material or after extraction from plants boast remarkable advantages, including heightened efficacy, minimal toxicity, infection-free properties, and scarce side effects, all complemented with excellent biocompatibility.…”

Dissolved Carbon Dioxide: The Lifespan of Staphylococcus aureus and Enterococcus faecalis in Bottled Carbonated Mineral Water

“…Staphylococcus aureus were cultivated on blood agar and exposed to pure CO 2 , a standard anaerobic gas, or air, over a period of 24 h [ 4 ]. Recent studies on methicillin-resistant S. aureus (MRSA) were concerned with antibacterial material and pathogeny [ 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. The survival of Escherichia coli , P. aeruginosa , Enterobacter cloacae , and Klebsiella pneumonia were investigated in bottled still water and demonstrated a reduction of colony-forming units (CFUs) over a period of twenty days [ 13 ].…”