Green synthesis, characterization and biomedical applications of Centella asiatica-derived carbon dots

Thokchom, Bothe; Bhavi, Santosh Mallikarjun; Abbigeri, Megha Basavaraj; Shettar, Arun Kashivishwanath; Yarajarla, Ramesh Babu

doi:10.1007/s42823-023-00505-3

Cited by 12 publications

(6 citation statements)

References 61 publications

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“…The biocompatibility, radical‐scavenging abilities, and protein stabilization of citric acid‐derived and N‐doped CDs were enhanced by their small size and the presence of functional groups, such as −OH and −COOH on the surface of the bio‐derived CDs. Recently, Thokchom et al [53] . (2023) suggested that CDs could strongly bind to the BSA protein through hydrogen bonds and van der Waals interactions, which protected the protein from thermal denaturation.…”

Section: Resultsmentioning

confidence: 99%

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Commercial Wheat and Oat Flour Derived Fluorescent Carbon Quantum Dots through Sustainable Hydrothermal Synthesis and Their Biological Activities

Kuppusamy,

Kim,

Park

et al. 2024

ChemistrySelect

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In this study, we utilized processed edible commercial wheat and oat flours to synthesize highly fluorescent carbon dots using green hydrothermal synthesis. The as‐synthesized carbon quantum dots (CQDs) were characterized using UV‐vis spectrophotometry, photoluminescence spectrophotometry, high‐resolution transmission electron microscopy, and Fourier transform infrared spectroscopy. The synthesized wheat‐CQDs (W‐CQDs) and oat‐CQDs (O‐CQDs) had monodispersed spherical shapes with average sizes of 3–8 and 5–10 nm, respectively, and exhibited strong fluorescence intensity and excitation‐dependent photoluminescence. The W‐CQDs and O‐CQDs were highly dispersed in aqueous solutions and exhibited bright green and blue fluorescence, respectively, under UV light (λex=380 and 370 nm). Both CQDs contained abundant functional groups, including amino, hydroxyl, and carboxyl groups, which may play a major role in biological applications. The W‐CQDs and O‐CQDs showed good free‐radical scavenging capacity in the 2,2‐diphenylpicrylhydrazyl (DPPH) and H2O2 assays. The anti‐inflammatory activities of W‐CQDs and O‐CQDs were more effective than that of the standard, with EC50 values of 1.8 and 1.3 mg/mL, respectively, in the protein denaturation assay. Additionally, the CQDs exhibited low cytotoxicity toward NIH3T3 fibroblast and human HeLa cervical cancer cells when treated with a 5‐mg/mL concentration. Therefore, green‐synthesized W‐CQDs and O‐CQDs are easily available, less cytotoxic, and applicable to therapeutic and cell nanocarrier purposes.

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Section: Resultsmentioning

confidence: 99%

“…The in‐vitro anti‐inflammatory assay for CDs was conducted by inhibiting the BSA protein denaturation method reported by Thokchom et al [53] . (2023), with slight modifications.…”

Section: Methodsmentioning

confidence: 99%

Commercial Wheat and Oat Flour Derived Fluorescent Carbon Quantum Dots through Sustainable Hydrothermal Synthesis and Their Biological Activities

Kuppusamy,

Kim,

Park

et al. 2024

ChemistrySelect

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“…The synthesis of CQDs was carried out as per a previously reported simple microwave-assisted method, with slight modification 14 . 20 mL of aqueous leaf extract was taken in a 250 mL conical flask and irradiated in the microwave oven (IFB 20PG3S) for 30 s, while avoiding spillage, and cooled for 1 min.…”

Section: Methodsmentioning

confidence: 99%

“…The traditional synthesis of CQDs involves the use of toxic and hazardous chemicals, such as organic solvents, strong acids, and bases, which can pose a risk to human health and the environment. In contrast, green synthesis methods use natural, renewable, and biodegradable resources, such as waste biomass 13 , plant extracts 14 , fruit juices 15 , and microorganisms 16 , to produce CQDs without the need for toxic chemicals, which ensures a more promising sustainable and eco-friendly alternative. Thus, fabricating and functionalizing CQDs with medicinal plants is a new progress which has potential in the field of biomedical diagnosis.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, fabricating and functionalizing CQDs with medicinal plants is a new progress which has potential in the field of biomedical diagnosis. Medicinal plants like neem 17 , turmeric 18 , ginger 19 , aloe vera 20 , tulsi 21 , brahmi 14 and many others belong to this list. This combinational study cumulates the unique properties of plant and CQDs and enhance their applicability.…”

Section: Introductionmentioning

confidence: 99%

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Microwave-assisted synthesis, characterization and in vitro biomedical applications of Hibiscus rosa-sinensis Linn.-mediated carbon quantum dots

Yalshetti,

Thokchom,

Bhavi

et al. 2024

Sci Rep

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In recent years, carbon quantum dots (CQDs) have garnered considerable attention as a promising material for biomedical applications because of their unique optical and biological properties. In this study, CQDs were derived from the leaves of Hibiscus rosa-sinensis Linn. via microwave-assisted technique and characterized using different techniques such as ultraviolet–visible, Fourier transform infrared, fluorescence spectrometry, X-ray diffraction, dynamic light scattering, transmission electron microscopy and energy-dispersive X-ray spectroscopy. Subsequently, their potential for biomedical applications was investigated through in vitro assays assessing scratch healing, anti-inflammatory, antibacterial, and cytotoxicity properties. It was found that the CQDs were fluorescent, polycrystalline, quasi-spherical, ~ 12 nm in size with presence of –OH and –COOH groups on their negatively charged surfaces, and demonstrated good anti-inflammatory by inhibiting protein denaturation, cyclooxygenase-2 and regulating inflammatory cytokines. The CQDs also exhibited antimicrobial activity against Klebsiella pneumoniae and Bacillus cereus, good biocompatibility, along with excellent promotion of cell proliferation in vitro, indicating their potential as a anti-inflammatory and wound healing material. The properties were more enhanced than their precursor, H. rosa-sinensis leaf extract. Hence, the CQDs synthesized from the leaves of H. rosa-sinensis can serve as a potential biomedical agent.

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Carbon Dots: A Review with Focus on Sustainability

Ren,

Opoku,

Tang

et al. 2024

Advanced Science

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Carbon dots (CDs) are an emerging class of nanomaterials with attractive optical properties, which promise to enable a variety of applications. An important and timely question is whether CDs can become a functional and sustainable alternative to incumbent optical nanomaterials, notably inorganic quantum dots. Herein, the current CD literature is comprehensively reviewed as regards to their synthesis and function, with a focus on sustainability aspects. The study quantifies why it is attractive that CDs can be synthesized with biomass as the sole starting material and be free from toxic and precious metals and critical raw materials. It further describes and analyzes employed pretreatment, chemical‐conversion, purification, and processing procedures, and highlights current issues with the usage of solvents, the energy and material efficiency, and the safety and waste management. It is specially shown that many reported synthesis and processing methods are concerningly wasteful with the utilization of non‐sustainable solvents and energy. It is finally recommended that future studies should explicitly consider and discuss the environmental influence of the selected starting material, solvents, and generated byproducts, and that quantitative information on the required amounts of solvents, consumables, and energy should be provided to enable an evaluation of the presented methods in an upscaled sustainability context.

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Green synthesis, characterization and biomedical applications of Centella asiatica-derived carbon dots

Cited by 12 publications

References 61 publications

Commercial Wheat and Oat Flour Derived Fluorescent Carbon Quantum Dots through Sustainable Hydrothermal Synthesis and Their Biological Activities

Commercial Wheat and Oat Flour Derived Fluorescent Carbon Quantum Dots through Sustainable Hydrothermal Synthesis and Their Biological Activities

Microwave-assisted synthesis, characterization and in vitro biomedical applications of Hibiscus rosa-sinensis Linn.-mediated carbon quantum dots

Carbon Dots: A Review with Focus on Sustainability

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