Green Synthesis, Characterization, and Assessment of Iron Oxide Nanoparticles' Antibacterial Activity Using Blumea lacera Root Extract

Abstract

Iron and copper nanoparticles produced through green methods underwent analysis using UV-visible absorption spectrophotometry, X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and EDS. Findings indicated that iron oxide nanoparticles displayed an irregular spherical shape, with sizes ranging from 15.73 nm to 32.37 nm. The antimicrobial efficacy of iron and copper oxide nanoparticles synthesized through green techniques was evaluated against four bacterial strains causing human diseases. Results showed that these nanoparticles exhibited the highest antibacterial activity at a concentration of 20 mm/disc, while their effectiveness was lowest at 13 mm/disc concentrations. This study underscores the potential of iron and copper oxide nanoparticles produced via environmentally friendly methods to serve as antibacterial agents.