Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles mediated by Eucalyptus camaldulensis leaf extract

Mohammed, Afrah E.

doi:10.1016/s2221-1691(15)30373-7

Cited by 93 publications

(36 citation statements)

References 16 publications

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“…11,12 Accordingly, biological methods of AgNPs synthesis got more consideration since there is no use of any toxic chemicals in the synthesis process and they cost less, eco-friendly. 13 The AgNPs biosynthesis is a simple process and extensive amounts. Nanoparticles (NPs) can be set up in a short time and the plant extract is considered as an energizing branch and used as a reducing and stabilizing agent in the biosynthesis processes.…”

Section: Introductionmentioning

confidence: 99%

<p>Nanoformulation of Biogenic Cefotaxime-Conjugated-Silver Nanoparticles for Enhanced Antibacterial Efficacy Against Multidrug-Resistant Bacteria and Anticancer Studies</p>

Halawani¹,

Hassan²,

El-Rab³

2020

IJN

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Due to the expanded bacterial genetic tolerance to antibiotics through different mechanisms, infectious diseases of MDR bacteria are difficult for treatment. Consequently, we synthesized drug conjugated nanoparticles to dissolve this problem. Moreover, the present study aims to display the cell death status treated with cefotaxime-CS-AgNPs and also, apoptosis pathways of human RPE-1 normal cells and human MCF-7 breast cancer cells. Methods: Here, we demonstrate the possibility to synthesize AgNPs and conjugate them with cefotaxime to survey the probability of cefotaxime-CS-AgNPs as an antimicrobial agent against cefotaxime-resistant strains E. coli and MRSA. Results: TEM showed the size of AgNPs, CS-AgNPs and cefotaxime-CS-AgNPs ranged from 7.42 to 18.3 nm, 8.05-23.89 nm and 8.48-25.3 nm, respectively, with a spherical shape. The cefotaxime-CS-AgNPs enhanced the high antimicrobial properties compared to AgNPs or pure antibiotic. The MIC of Cefotaxime-CS-AgNPs ranged from 3 µg/mL to 8 µg/mL against tested E. coli and MRSA bacteria. Consequently, the highest reduction in the MIC of cefotaxime-CS-AgNPs was noted against tested strains ranging from 22% to 96%. Comparing cefotaime-CS-AgNPs to AgNPs we showed that cefotaime-CS-AgNPs have no cytotoxic effect on normal cells at even 12 µg/mL for 24 hrs. The IC50 for the AgNPs and cefotaxime-CS-AgNPs was 12 µg/mL for human RPE-1 normal cells and human MCF-7 breast cancer cell lines. The pro-apoptotic genes p53, p21, and Bax of cancer cell lines significantly upregulated followed by downregulated by anti-apoptotic gene Bcl-2 after 48 hrs at 24 µg/mL, and this concentration represents the most effective dose. Conclusion: Results enhanced the conjugating utility in old unresponsive cefotaxime to AgNPs to restore its efficiency against previous strains and demonstrated potential therapeutic applications of cefotaxime-CS-AgNPs. Moreover, this research gives remarkable insights for designing nanoscale delivery and curative systems that have a pronounced cytotoxic activity on cancer cells and are safe to normal cells.

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Section: Introductionmentioning

confidence: 99%

<p>Nanoformulation of Biogenic Cefotaxime-Conjugated-Silver Nanoparticles for Enhanced Antibacterial Efficacy Against Multidrug-Resistant Bacteria and Anticancer Studies</p>

Halawani¹,

Hassan²,

El-Rab³

2020

IJN

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“…But, the formation of complexes for silver ions is limited and the effect of the silver ions somehow remains only for a short period (Méndez-Vilas 2011). Yet, this drawback has been resolved by the application of the intact silver nanoparticles which have greater antibacterial properties by promoting the synthesis of reactive oxygen species such as hydrogen peroxide (Mohammed 2015). In addition to antibacterial activity of the silver nanoparticles, a complete disruption of the bacterial membrane of Escherichia coli cells was observed after few minutes in contact with silver nanoparticles under TEM analysis (Raffin et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of nanoparticles and silver nanoparticles

Keat

Aziz

Eid

et al. 2015

Bioresour. Bioprocess.

291

123

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In this century, the development of nanotechnology is projected to be the establishment of a technological evolutionary of this modern era. Recently, nanotechnology is one of the most active subjects of substantial research in modern material sciences and hence metal nanoparticles have a great scientific interest because of their unique optoelectronic and physicochemical properties with applications in diverse areas such as electronics, catalysis, drug delivery, or sensing. Nanotechnology provides an understanding on fundamental properties of objects at the atomic, molecular, and supramolecular levels. Besides, nanotechnology also leads an alternative technological pathway for the exploration and revolution of biological entities, whereas biology provides role models and biosynthetic constituents to nanotechnology. The findings of this review are important to provide an alternative for the green synthesis of silver nanoparticles. It showed more cost-effective and environmental friendly application as well as easier for large production, with relation to the properties of silver nanoparticles as antimicrobial, can be served well as an alternative antiseptic agent in various fields. Typically, silver nanoparticles are smaller than 100 nm and consist of about 20-15,000 silver atoms. Due to the attractive physical and chemical properties of silver at the nanoscale, the development of silver nanoparticles is expanding in recent years and is nowadays significant for consumer and medical products.

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“…Methods that use biological materials, such as fungi, biomolecules, bacteria, and extracted plants, have been abundantly utilized for Ag NP synthesis (5)(6)(7)(8). Among these methods, the use of plant extracts has experienced a rapid advance in the last decade because of advantages such as rapid preparation, eco-friendliness, nonpathogenic effects, non-toxicity, economically feasible procedures, and provision of natural reducing agents of plant extracts for the stabilization of Ag NPs (8)(9)(10)(11)(12)(13)(14)(15)(16). Consisting of biomolecules like proteins, amino acids, enzymes, polysaccharides, alkaloids, tannins, phenolics, saponins, terpinoids, and vitamins, plant extracts have been used as reducing and stabilizing agents for converting Ag ions to Ag NPs via a method known as "green synthesis."…”

Section: Introductionmentioning

confidence: 99%

Green synthesis, characterization and antibacterial activity of silver nanoparticles from root extract of Lepidium draba weed

Benakashani

Allafchian

Jalali

2017

Green Chemistry Letters and Reviews

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Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles mediated by Eucalyptus camaldulensis leaf extract

Cited by 93 publications

References 16 publications

<p>Nanoformulation of Biogenic Cefotaxime-Conjugated-Silver Nanoparticles for Enhanced Antibacterial Efficacy Against Multidrug-Resistant Bacteria and Anticancer Studies</p>

<p>Nanoformulation of Biogenic Cefotaxime-Conjugated-Silver Nanoparticles for Enhanced Antibacterial Efficacy Against Multidrug-Resistant Bacteria and Anticancer Studies</p>

Biosynthesis of nanoparticles and silver nanoparticles

Green synthesis, characterization and antibacterial activity of silver nanoparticles from root extract of Lepidium draba weed

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