Green synthesis and physiochemical characterization of nickel oxide nanoparticles: Interaction studies with Calf thymus DNA

Abstract

One of the most promising applications of nanomaterials is that of nanobiosensors, using biomolecules such as nucleic acids as receptors. This study aimed to synthesize nickel oxide nanoparticles (NiO NPs) by an environmentally friendly green synthesis, using the extract of the herb Coriandrum sativum (coriander). The synthesized NPs were characterized using UV–Visible spectroscopy, Fourier transform infrared spectroscopy, X‐ray diffraction, X‐ray photon spectroscopy, field emission scanning electron microscopy coupled with energy dispersive spectroscopy, dynamic light scattering, zeta potential and transmission electron microscopy. All results confirmed the synthesis of pure, spherical, positively charged NiO NPs of around 95 nm in diameter with prominent hydroxyl groups attached to the surface. Furthermore, interaction studies of synthesized NiO NPs with calf thymus DNA (CT DNA) were performed using UV–Visible spectroscopy, UV–thermal melting, circular dichroism, and fluorescence spectroscopy. CT DNA served as a substitute for nucleic acid biosensors. All experimental studies indicated that the NiO NPs bound electrostatically with CT DNA. These studies may facilitate exploring the potential of NiO NP–nucleic acid conjugated materials to be used as nanobiosensors for various applications, especially in pharmacological, epidemiological, and environmental diagnostic applications, and in detection.

A smart DOPO‐containing decoration armed on Salen‐polyphosphazene toward high‐efficient flame retardancy for epoxy thermoset

“…Besides, for Ni 2p spectra, two main peaks are centered at 874.2 and 856.6 eV are Ni 2p1/2 and Ni 2p3/2 of NiO, respectively, and meanwhile, the peaks appearing at 878.9 and 863.2 eV correspond to the satellite peaks of NiO. [ 46–49 ] The chemical bonds in the residue of DK@Salen‐PZN‐Ni/EP composite imply the existence of oxides as well as the formation of some complex substances derived from the interaction between DK and Salen‐PZN‐Ni (e.g., SiOP and SiP), representing that DK modification on enhancing charring formation and retarding fire spread in condensed phase depend on combustible chemical reaction as well as their physical barrier effect. [ 50 ] However, these specific combustion products in residue are still ambiguous known.…”

A novel flame retardant consisting of functionalized Salen-Ni based polyphosphazene microspheres

“…Additionally, the peaks present at 881.3 and 862.7 eV belong to the satellites of NiO. [39][40][41] The above illustrates that Salen-PZN-Ni@DHPP-PTS has excellent flame retardancy and smoke suppression effects.…”

Green Nanotechnology: Recent Research on Bioresource-Based Nanoparticle Synthesis and Applications

“…e NiO NPs bonded electrostatically with CT DNA in all experimental investigations. It was concluded that NiO NP-nucleic acid coupled materials can be utilized as nanobiosensors for a variety of applications in pharmacy, environment, and detection [135]. Additionally, NiO NPs were used to enhance glucose sensing.…”