Green Synthesis and Characterization of Biologically Synthesized and Antibiotic-Conjugated Silver Nanoparticles followed by Post-Synthesis Assessment for Antibacterial and Antioxidant Applications

Abstract

The paper presents the antibacterial and antioxidant activities of silver nanoparticles (AgNPs) when conjugated with two antibiotics levofloxacin and ciprofloxacin as well as biologically synthesized nanoparticles from Moringa oleifera and Curcuma longa. Leaves of Moringa and powder of Curcuma were used in the green synthesis of silver nanoparticles. Ultraviolet−visible spectroscopy (UV), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) were used for the characterization of the synthesized silver nanoparticles. Comparison of levofloxacin and ciprofloxacin and their conjugated AgNPs was also studied for antibacterial and antioxidant activity. The synthesis of Moringa-AgNPs, turmeric-AgNPs, levofloxacin-AgNPs, and ciprofloxacin-AgNPs was confirmed by UV spectroscopy. An absorption peak value of 400−450 nm was observed, and light to dark brown color indicated the synthesis of AgNPs. Moringa-AgNPs revealed high antioxidant activity (80.3 ± 3.14) among all of the synthesized AgNPs. Lev-AgNPs displayed the highest zone of inhibition for Staphylococcus aureus, while in Escherichia coli, Cip-AgNPs showed high antibacterial activity. Furthermore, AgNPs synthesized using green methods exhibit high and efficient antimicrobial activities against two food-borne pathogens. Biologically synthesized nanoparticles exhibited antibacterial activity against E. coli (13.73 ± 0.46 with Tur-AgNPs and 13.53 ± 0.32 with Mor-AgNPs) and S. aureus (14.16 ± 0.24 with Tur-AgNPs and 13.36 ± 0.77 with Mor-AgNPs) by using a well diffusion method with significant shrinkage and damage of the bacterial cell wall, whereas antibiotic-conjugated nanoparticles showed high antibacterial activity compared to biologically synthesized nanoparticles with 14.4 ± 0.37 for Cip-AgNPs and 13.93 ± 0.2 for Lev-AgNPs for E. coli and 13.3 ± 0.43 for Cip-AgNPs and 14.33 ± 0.12 for Lev-AgNPs for S. aureus. The enhanced efficiency of conjugated silver nanoparticles is attributed to their increased surface area compared to larger particles. Conjugation of different functional groups contributes to improved reactivity, creating active sites for catalytic reactions. Additionally, the precise control over the size and shape of greensynthesized nanoparticles further augments their catalytic and antibiotic activities.

Development of Biologically Active Phytosynthesized Silver Nanoparticles Using Marrubium vulgare L. Extracts: Applications and Cytotoxicity Studies

“…In particular, several new peaks appear in the NP FTIR spectra, while others show a significant increase in intensity as compared to the crude extracts: 1639/1651 cm −1 , 1608 cm −1 (more visible in MM-AgNP), 1516/1515 and 1450/1453, 1272/1248, and 1172/1173, respectively. Literature data [50] assign the apparition of the FTIR peaks 1172/1173 and 1272/1248 cm −1 to the presence of AgNPs. The increase observed for the peak at 3363/3331 cm −1 is most probably due to the presence of silver nanoparticles, as in [48].…”