Green Synthesis and Characterization of Silver NPs Using Oyster Mushroom Extract For Antibacterial Efficacy

Abstract

The present study was aimed to synthesize silver nanoparticles (AgNPs)using the extract of oyster mushroom Pleurotuscitrinopileatus and its use in antibacterial testing. This green synthesis approach of silver NPs is very fast, simple, environmentally friendly and economical. The initial confirmation of silver NPs synthesis was observed with the alteration of the colour of the solution from colourless to wine red. The prepared nano-material was further characterized by UV-Visible spectrophotometer, and transmission electron microscopy (TEM). The TEM revealed particle size of synthesized AgNPsbetween the range of 11-13 nm. The bactericidal efficacy of silver NPstested against Escherichia coli confirmed the lowest 50 µg/L concentration of silver NPs bactericidal. Therefore based the observations of the study silver NPsat the level of its 50 µg/L can be used for the purposes of potential water disinfection, killing of bacteria, disinfection of medical equipments, wound washings, preservation of food stuffs and in hand sanitization.

Five nanometer size highly positive silver nanoparticles are bactericidal targeting cell wall and adherent fimbriae expression

“…In a separate study, Kim et al 43 documented AgNPs to form pits within the cell membrane, thereby increasing cell permeability. Another study reported antibacterial potential of AgNPs synthesized using a mushroom ( Pleurotus sajor-caju ) extract, but the surface moiety was not determined in the study 44 . Research also indicates Ag + ions could inhibit bacterial growth by disrupting DNA replication and inactivating thiol groups in many enzymes 43 .…”

Green Synthesis of Silver Nanoparticles from Macrocybe gigantea and its Effect Against Food Borne Pathogens

“…For the synthesis of NPs, the M. gigantea basidiocarps were oven dried at 40 • C for 2 hours after being rinsed with doubledistilled water. 50mL of hot, boiled, distilled water was added to 6g of powder and allowed to cool at room temperature before being filtered using Whatman-4 filter paper and employed as mushroom extract (15).…”

The antibacterial and toxicological studies of mycosynthesis silver nanoparticles by isolated phenols from agaricus bisporus

“…AgNPs are known for their high surface-to-volume ratio as well as antimicrobial and antifungal activities agents in the health sector and cosmetic industry due to their unique morphology and small size in the range from 1 to 100 nm [8][9][10]. Ongoing efforts are being made to investigate the anti-bacterial properties of AgNPs [11]. Microscopic organisms like bacteria, viruses, yeasts, and molds are often the cause of infections in human beings in their living environments.…”

“…Among these, phenol contains antioxidant [22] and antibacterial [23] compounds and plays the dual role of reducing the ions in Ag and acting as a capping agent of AgNPs surface [24]. More specifically, the mushroom extract has been used for the mycosynthesis of AgNPs because of their antimicrobial activity against pathogenic bacteria due to their critical function in destroying the bacteria's DNA and cellular macromolecules [11,19,25].…”