Green synthesis and characterization of Camellia sinensis mediated silver nanoparticles for antibacterial ceramic applications

Göl, Fatma; Aygün, Ayşenur; Seyrankaya, Abdullah; Gür, Tuğba; Yenikaya, Cengiz; Şen, Fatih

doi:10.1016/j.matchemphys.2020.123037

Cited by 92 publications

(29 citation statements)

References 62 publications

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“…Antibacterial activities of green AgNPs was reported at a very low dose. 4 , 30 AgNPs were found to be more effective toward Gram negative bacteria than Gram positive bacteria because of the presence of their thinner outer membrane, the peptidoglycan layer. 29 In the present study, we have checked the antibacterial activities of the newly synthesized Ag/AgCl-NPs against pathogenic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Zizyphus mauritiana Fruit Extract-Mediated Synthesized Silver/Silver Chloride Nanoparticles Retain Antimicrobial Activity and Induce Apoptosis in MCF-7 Cells through the Fas Pathway

et al. 2020

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Recently, green synthesis of silver/silver chloride nanoparticles (Ag/AgCl-NPs) has gained a lot of interest because of the usage of natural resources, rapidness, eco-friendliness, and benignancy. Several researchers reported that silver-based biogenic NPs have both antimicrobial and anticancer properties. In the present study, Ag/AgCl-NPs were synthesized from Zizyphus mauritiana fruit extract, and their antibacterial, antifungal, and antiproliferative mechanisms against human MCF-7 cell lines were evaluated. Synthesis of Ag/AgCl-NPs from the Z. mauritiana fruit extract was confirmed by the changes of color and a peak of the UV–visible spectrum at 428 nm. The nanoparticles were characterized by transmission electron microscopy, energy dispersive X-ray, X-ray powder diffraction, thermal gravimetric analysis, atomic force microscope, and Fourier transform infrared. Antibacterial activity was checked against four pathogenic bacteria and two fungi. Cytotoxicity was checked against human breast cancer cell line (MCF-7) and mice Ehrlich ascites carcinoma (EAC) cells by MTS assay and clonogenicity assay. Cell morphology of the control and nanoparticle-treated MCF-7 cells were checked by Hoechst 33342, YF488-Annexin V, and caspase-3 substrates. The level of reactive oxygen species (ROS) was studied by using 2′,7′-dichlorofluorescein-diacetate staining. Real-time polymerase chain reaction was used for gene expression. Synthesized nanoparticles were heat stable cubic crystals with an average size of 16 nm that contain silver and chlorine with various functional groups. The synthesized Ag/AgCl-NPs inhibited the growth of three pathogenic bacteria ( Bacillus subtilis , Shigella boydii, and Escherichia coli ) and two fungi ( Aspergillus niger and Trichoderma spp.). Ag/AgCl-NPs inhibited the growth of MCF-7 and EAC cells with the IC 50 values of 28 and 84 μg/mL, respectively. No colony was formed in MCF-7 cells in the presence of these nanoparticles as compared with control. Ag/AgCl-NPs induced apoptosis and generated ROS in MCF-7 cells. The expression level of FAS, FADD, and caspase-8 genes increased several folds with the decrease of PARP gene expression. These results demonstrated that the anti-proliferation activity of Ag/AgCl-NPs against MCF-7 cells resulted through ROS generation and induction of apoptosis through the Fas-mediated pathway.

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Section: Discussionmentioning

confidence: 99%

Zizyphus mauritiana Fruit Extract-Mediated Synthesized Silver/Silver Chloride Nanoparticles Retain Antimicrobial Activity and Induce Apoptosis in MCF-7 Cells through the Fas Pathway

et al. 2020

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“…Among the techniques, green synthesis of silver/silver chloride NPs (Ag/AgCl‐NPs) gained a lot of interest due to the usage of natural resources, rapidness and eco‐friendliness 4 . Various biomolecules such as enzymes, proteins, flavonoids, terpenoids and cofactors are present in the fruit, bark, root, and leave of plant those act as reducing and capping agents of silver 3‐8 . Some of the synthesized nanoparticles showed potent antiproliferative effect against different cancer cell lines 9‐13 .…”

Section: Introductionmentioning

confidence: 99%

Biogenic silver/silver chloride nanoparticles inhibit human glioblastoma stem cells growth in vitro and Ehrlich ascites carcinoma cell growth in vivo

Kabir

Dai

Nurujjaman

et al. 2020

J Cellular Molecular Medi

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The importance of biogenic silver/silver chloride nanoparticles has become increasing day by day. In the present study, silver/silver chloride nanoparticles (Ag/AgCl‐NPs) were synthesized from Kaempferia rotunda tuberous rhizome extract to evaluate the antiproliferative activity against human glioblastoma stem cells (GSCs) in vitro and Ehrlich ascites carcinoma (EAC) cells in vivo in mice. Synthesis of nanoparticles was confirmed by colour change and UV‐visible spectrum and characterized by TEM, XRD, TGA, AFM and FTIR. K rotunda and recently synthesized Zizyphus mauritiana fruit extract‐mediated Ag/AgCl‐NPs inhibited 77.2% and 71% of GSCs growth at 32 µg/mL concentration with the IC50 values of 6.8 and 10.4 µg/mL, respectively. Cell morphological studies and caspase‐3 immunofluorescence assay revealed that both biogenic nanoparticles induced apoptosis in GSCs. Expression levels of several genes were checked by real‐time PCR after treatment with K rotunda tuberous rhizome‐mediated Ag/AgCl‐NPs. PARP, EGFR, NOTCH2 and STAT3 gene expression were decreased with the increase of NFκB, TLR9, IL1, TNFα, IKK and p21 gene that would be the cause of induction of apoptosis in GSCs. The cell cycle arrest at G2/M phase was confirmed by flow cytometric assay. Both nanoparticles were injected intraperitoneally to rapidly growing EAC cells for 5 consecutive days. Approximately, 32.3% and 55% EAC cells growth were inhibited by K rotunda tuberous rhizome‐mediated Ag/AgCl‐NPs at 6 and 12 mg/kg/day doses, respectively while only 20% cell growth inhibition was monitored at 12 mg/kg/day dose of Z mauritiana‐mediated Ag/AgCl‐NPs. From the above results, it can be concluded that presently synthesized nanoparticles would be a potent anticancer agent.

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“…Preliminary studies were performed to evaluate the effect of the green tea concentration in the reaction mixture on the synthesis of AgNPs under alkaline conditions. Three concentrations were tested (0.7, 3.4, and 6.0 mg mL −1 ) following the common conditions for biogenic synthesis of nanoparticles by green tea extracts 14,24 . According to the results obtained, a high LSPR intensity was observed when the concentration of green tea was above 0.7 mg mL −1 , showing the favorable effect when the reducing compounds concentration increases (Supporting Information Fig.…”

Section: Resultsmentioning

confidence: 97%

“…In the main, the use of natural, renewable, and widely distributed sources, such as plants, roots, and fungi, that have compounds with reducing and stabilizing properties, including polyphenols, flavonoids, carbohydrates, and vitamins, is receiving great attention 12,13 . The use of green tea for generation of AgNPs was recently proposed because of the synergistic effect between both the antioxidant and anti‐inflammatory properties of tea components and antimicrobial effects of nanoparticles, leading to biomedical and industrial applications 12,14 . Nevertheless, the use of AgNPs obtained from green tea extracts as a unique source of reducing and stabilizing compounds has not yet been explored as a sensor for TC determination.…”

Section: Introductionmentioning

confidence: 99%

Green‐tea‐synthesized silver nanoparticles as a sensing platform for determination of tetracycline in honey samples

2021

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BACKGROUND Silver nanoparticles (AgNPs) can be easily obtained in aqueous solution by chemical reduction using appropriate reducing agents and stabilizers. The development of environmentally friendly methods using non‐toxic solvents and reagents has become an alternative for the synthesis of these particles and their future application as sensor probes for agricultural products. In this work, a straightforward method based on green tea extracts as reducing and capping agent is proposed for the synthesis of AgNPs, followed by their evaluation as a sensing platform for determination of tetracycline in honey samples. RESULTS Highly stable nanoparticles were easily obtained by combining green tea aqueous extracts and ultrasound irradiation for 2 min. The as‐synthesized AgNPs, spherical in shape and with average size of 8.5 nm, were evaluated for determination of tetracycline by following the changes on the localized surface plasmon resonance band at 450 nm induced by the presence of this antibiotic at pH 5.8. The method was successfully applied in the concentration range between 200 and 800 μg L−1 with R2 > 0.996 and limit of detection of 52.7 μg L−1. Multiple honey samples were analyzed, and the recovery values obtained ranged between 82.8% and 116%, with relative standard deviation values lower than 6.69%. CONCLUSION The results obtained demonstrate that the AgNPs synthesized using just green tea extracts represent a promising and sustainable alternative tool for the cost‐effective determination of tetracycline antibiotics in honey. © 2021 Society of Chemical Industry

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Green synthesis and characterization of Camellia sinensis mediated silver nanoparticles for antibacterial ceramic applications

Cited by 92 publications

References 62 publications

Zizyphus mauritiana Fruit Extract-Mediated Synthesized Silver/Silver Chloride Nanoparticles Retain Antimicrobial Activity and Induce Apoptosis in MCF-7 Cells through the Fas Pathway

Zizyphus mauritiana Fruit Extract-Mediated Synthesized Silver/Silver Chloride Nanoparticles Retain Antimicrobial Activity and Induce Apoptosis in MCF-7 Cells through the Fas Pathway

Biogenic silver/silver chloride nanoparticles inhibit human glioblastoma stem cells growth in vitro and Ehrlich ascites carcinoma cell growth in vivo

Green‐tea‐synthesized silver nanoparticles as a sensing platform for determination of tetracycline in honey samples

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