Green renewable energy production from Myxosarcina sp.: media optimization and assessment of biodiesel fuel properties

Abstract

Renewable energy resources like biomass from plant and algae have gained more interest for biodiesel production as an energy source to reduce the consumption of fossil fuels and elevated global warming. In this study, Myxosarcina sp., an unicellular cyanobacterium was evaluated for higher biomass and lipid production by the supplementation of sugar industrial waste (SIW), sodium chloride (NaCl) and media optimization with response surface methodology (RSM) for biodiesel production. The outcome of the findings showed that biomass and lipid productivities of 28.5 ± 2.4 (1.2 fold) and 3.4 ± 0.2 (1.3 fold) mg/L/day were observed in the BG-11 media supplemented with SIW than control (24 ± 1 and 2.6 ± 0.4 mg/L/day). However hyper lipid content 20.6 ± 1.8 % (1.5 fold) was achieved by RSM optimized media including NaCl at 0.8 M, SIW at 2 mL/L, sodium nitrate (NaNO3) at 2.5 g/L and magnesium sulphate (MgSO4) at 0.075 g/L than control (13.6 ± 1.4 %). Fatty acid characterization by GC-MS anlysis revealed that Myxosarcina sp. yielded 39 ± 5.3 % of saturated fatty acids (SFA) and 61 ± 5.3 % of mono unsaturated fatty acid (MUFA) and its biodiesel fuel properties evaluated using empirical equations showed that almost all the properties calculated were in concurrence with the national and international biodiesel standards EN 14214 (Europe), ASTM D6751-02 (US) and IS 15607 (India). Thus, Myxosarcina sp. can be utilized as the environmental friendly biodiesel feedstock for high quality biodiesel production in the current scenario for the escalating energy demand.

Agro‐Industrial Waste Based Phycocyanin Production from Oscillatoria sp. 50 A: daf‐16 Modulating Effect in Caenorhabditis elegans and p53 Dependent Apoptosis in HeLa cells

“…Cyanobacteria are well known for the utilization of the industrial wastes for their luxurious growth and development using cheap nutrient requirements while their biomasses thus generated are widely applicable in fields such as biofuels, bioremediation, biofertilizers and bioremediation ,,. For a bioprocess technology like real‐time metabolite production from bacteria and algae, waste water supplementation has been successfully employed to make the process cost effective and eco‐friendly ,. Thus, this study was carried out to enhance the C‐PC production in Oscillatoria sp.…”

“…[1,8,9] For a bioprocess technology like real-time metabolite production from bacteria and algae, waste water supplementation has been successfully employed to make the process cost effective and eco-friendly. [14,17] Thus, this study was carried out to enhance the C-PC production in Oscillatoria sp. 50 A by combining the statistical RSM approach and industrial waste supplementation strategy.…”

“…Cyanobacteria are well known for the utilization of the polluted waste water from industries such as tannery, sugar, dye, brewery, paper, pharmaceutical, distilleries and fertilizers ,. Since the conventional media optimization methodology with one factor at a time is laborious, time consumable and less effective for bio‐metabolite production, statistical approach with multi‐dimensional strategy for optimization of the culture conditions would be highly suitable for evaluating the potential interaction among the various nutritional components to enhance the metabolite production ,,,…”

“…[8,9] Since the conventional media optimization methodology with one factor at a time is laborious, time consumable and less effective [10] for biometabolite production, statistical approach with multi-dimensional strategy for optimization of the culture conditions would be highly suitable for evaluating the potential interaction among the various nutritional components to enhance the metabolite production. [11,12,13,14] On the other hand, the therapeutic applications of C-PC is being employed for ameliorating effects in various tumors,pulmonary fibrosis and even alleviating complications in inflammation by cyclooxygenase inhibition. [15] However, the application of C-PC as a therapeutic agent in treating obese conditions are scarce and the role of C-PC for the reduction of high cholesterol and its anti-oxidant potential was reported in Syrian hamsters fed on hypercholesterol diet.…”

Potencial de cianobactérias cultivadas em meio suplementado com vinhaça para produção de biodiesel

“…Nessa etapa, buscou-se direcionar os ensaios para a identificação das principais características de crescimento de duas linhagens: Amazoninema brasiliense CMAA1602 e Planktothix mougeotii CMAA1564. A primeira foi selecionada devido a composição de ácidos graxos da biomassa quase que em sua totalidade composta por ácidos graxos saturados e monoinsaturados, favoráveis para a produção de biodiesel (ŘEZANKA et al, 2003;KNOTHE, 2009;JAWAHARRAJ et al, 2015). A segunda, foi selecionada devido ao maior acúmulo de biomassa observado durante o seu cultivo, quando comparado às demais linhagens.…”