Green Nut Oil or DHA Supplementation Restored Decreased Distribution Levels of DHA Containing Phosphatidylcholines in the Brain of a Mouse Model of Dementia

Abstract

Dementia is a major public health concern nowadays. Reduced levels of brain docosahexaenoic acid (DHA) and DHA-phosphatidylcholines (DHA-PCs) in dementia patients were reported previously. Recently, we have reported that supplementation of green nut oil (GNO) or DHA improves memory function and distribution levels of brain DHA in senescence accelerated mice P8 (SAMP8). GNO is extracted from Plukenetia volubilis seeds, and SAMP8 is a well-known model mouse of dementia. In this current study, we examined the results of GNO or DHA supplementation in the distribution levels of brain DHA-PCs in same model mouse of dementia using desorption electrospray ionization (DESI) mass spectrometry imaging (MSI). We observed significantly decreased distribution of brain DHA-PCs, PC (16:0_22:6), and PC (18:0_22:6) in SAMP8 mice compared to wild type mice, and GNO or DHA treatment restored the decreased distribution levels of PC (16:0_22:6) and PC (18:0_22:6) in the brain of SAMP8 mice. These results indicate that GNO or DHA supplementation can ameliorate the decreased distribution of brain DHA-PCs in dementia, and could be potentially used for the prevention and treatment of dementia.

Brain Transport of Docosahexaenoic Acid Across the Blood–Brain Barrier Decreases in Middle-Aged and Aged Mice

“…Several studies have demonstrated the relationship between brain DHA levels and events that occur with increased age. For example, patients with dementia have decreased brain DHA [27,33], and DHA supplementation improves the accumulation of brain DHA in a dementia mouse model [34,35]. Further, higher DHA intake is inversely correlated with the relative risk of AD [36].…”

The association between the clinical severity of heart failure and docosahexaenoic acid accumulation in hypertrophic cardiomyopathy

“…The DESI-IMS mass spectra were calibrated externally using a 500 µM sodium formate solution in 90% 2-propanol, prior to measurement. To obtain the maximum signal intensity from heart biopsy samples, DESI parameters were optimized as previously described [ 9 ] with slight modifications. All the candidate molecules corresponding to each targeted m/z were selected using the Human Metabolome Database ( https://hmdb.ca/ ) based on their mass accuracy and biological distribution.…”

The Association between the Clinical Severity of Heart Failure and Docosahexaenoic Acid Accumulation in Hypertrophic Cardiomyopathy

“…The DESI-IMS mass spectra were calibrated externally using a 500 µM sodium formate solution in 90% 2-propanol, prior to measurement. To obtain the maximum signal intensity from heart biopsy samples, DESI parameters were optimized as previously described [9] with slight modi cations. All the candidate molecules corresponding to each targeted m/z were selected using the Human Metabolome Database (https://hmdb.ca/) based on their mass accuracy and biological distribution.…”