Green Nanoparticles for Biomedical and Bioengineering Applications

Silva, Luciano; Vaz, Gabriela Mendes da Rocha; Pupe, Júlia Moreira; Chafran, Liana S.; Neto, Lucio Assis Araujo; Santiago, Thaís Ribeiro; Araujo, Thalita Fonseca; Polez, Vera Lúcia Perussi

doi:10.1007/978-981-15-0391-7_9

Cited by 7 publications

(5 citation statements)

References 165 publications

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“…A scalingup production strategy is an important step in converting achievements in nanobiotechnology processes from scientific publications into real technologies and commercial products. As a result, in order to solve a large-scale challenge, the scaling-up strategy for biosynthesis of NPs, which includes increasing the quality and quantities of nanoproducts based on laboratory scale results, requires more optimization, development, and validation 50 .…”

Section: Resultsmentioning

confidence: 99%

“…Antimicrobial sensitivity of biosynthetic ZnO NPs at various doses. Using an agar-well diffusion method, the antimicrobial sensitivity of the controllable biosynthetic ZnO NPs at different doses (50,100,150,200,250, and 300 µg/mL) was tested in vitro against a variety of multi-drug resistant human pathogens, namely: Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhimurium, Streptococcus pneumoniae, Candida albicans, Candida krusei and Candida tropicals. After incubation, the inhibitory zones that appeared were evaluated.…”

Section: Methodsmentioning

confidence: 99%

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Scaling-up strategies for controllable biosynthetic ZnO NPs using cell free-extract of endophytic Streptomyces albus: characterization, statistical optimization, and biomedical activities evaluation

EL-Moslamy

Elnouby

Rezk

et al. 2023

Sci Rep

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In this study, we identified a suitable precursor and good cellular compartmentalization for enhancing bioactive metabolites to produce biosynthetic zinc oxide nanoparticles (ZnO NPs). An effective medium for cultivating endophytic Streptomycesalbus strain E56 was selected using several optimized approaches in order to maximize the yield of biosynthetic ZnO NPs. The highest biosynthetic ZnO NPs yield (4.63 g/L) was obtained when pipetting the mixed cell-free fractions with 100 mM of zinc sulfate as a precursor. The generation of biosynthetic ZnO NPs was quickly verified using a colored solution (white color) and UV–Visible spectroscopy (maximum peak, at 320 nm). On a small scale, the Taguchi method was applied to improve the culture medium for culturing the strain E56. As a result, its cell-dry weight was 3.85 times that of the control condition. And then the biosynthesis of ZnO NPs (7.59 g/L) was increased by 1.6 times. Furthermore, by using the Plackett–Burman design to improve the utilized biogenesis pathway, the biosynthesis of ZnO NPs (18.76 g/L) was increased by 4.3 times. To find the best growth production line, we used batch and fed batch fermentation modes to gradually scale up biomass output. All kinetics of studied cell growth were evaluated during fed-batch fermentation as follows: biomass yield was 271.45 g/L, yield coefficient was 94.25 g/g, and ZnO NPs yield was 345.32 g/L. In vitro, the effects of various dosages of the controllable biosynthetic ZnO NPs as antimicrobial and anticancer agents were also investigated. The treatments with controllable biosynthetic ZnO NPs had a significant impact on all the examined multidrug-resistant human pathogens as well as cancer cells.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Scaling-up strategies for controllable biosynthetic ZnO NPs using cell free-extract of endophytic Streptomyces albus: characterization, statistical optimization, and biomedical activities evaluation

EL-Moslamy

Elnouby

Rezk

et al. 2023

Sci Rep

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“…De fato, diversos são os fatores bióticos e abióticos que podem acarretar alterações no metabolismo das plantas e outros organismos, dentre eles a pressão ambiental constante, incluindo o estresse hídrico 201 , a falta ou excesso de nutrientes essenciais 201 , mudanças no pH do solo ou outros ambientes 202 , ataques de herbívoros patógenos e parasitas 203 , competição entre espécies 204 , o excesso ou a falta de exposição à luz 205,206 Por fim, a nanotecnologia verde também oferece oportunidades no que tange à geração de novos conceitos relacionados ao uso de nanomateriais, já que propriedades novas emergem do uso de recursos biológicos nos processos de produção de nanomateriais [215][216][217][218][219][220][221][222][223][224]…”

Section: Desafios Atuais E Perspectivas Para O Uso De Nanomateriais O...unclassified

Capítulo 4 - Síntese verde de nanomateriais

Silva,

Carvalho,

Bonatto

et al. 2022

Nanotecnologia : Considerações Em Materiais, Saúde E Meio Ambiente

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“…On account of the properties sketched above, many biomedical uses of plant viral nanoparticles are emerging and have been reviewed in detail (Franzen and Lommel, 2009;Czapar and Steinmetz, 2017;Steele et al, 2017;Aljabali et al, 2018;Eiben et al, 2019;Hema et al, 2019;Benjamin et al, 2020;Chung et al, 2020;Silva et al, 2020). They include, among others, the directed delivery of imaging agents and therapeutics to target sites, e.g., via the blood stream to tumors or atherosclerotic lesions.…”

Section: Applicability Of Plant Viral Nanoscaffoldsmentioning

confidence: 99%

Field-Effect Sensors for Virus Detection: From Ebola to SARS-CoV-2 and Plant Viral Enhancers

Poghossian¹,

Jablonski

Molinnus

et al. 2020

Front. Plant Sci.

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Coronavirus disease 2019 (COVID-19) is a novel human infectious disease provoked by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Currently, no specific vaccines or drugs against COVID-19 are available. Therefore, early diagnosis and treatment are essential in order to slow the virus spread and to contain the disease outbreak. Hence, new diagnostic tests and devices for virus detection in clinical samples that are faster, more accurate and reliable, easier and cost-efficient than existing ones are needed. Due to the small sizes, fast response time, label-free operation without the need for expensive and time-consuming labeling steps, the possibility of real-time and multiplexed measurements, robustness and portability (point-of-care and on-site testing), biosensors based on semiconductor field-effect devices (FEDs) are one of the most attractive platforms for an electrical detection of charged biomolecules and bioparticles by their intrinsic charge. In this review, recent advances and key developments in the field of label-free detection of viruses (including plant viruses) with various types of FEDs are presented. In recent years, however, certain plant viruses have also attracted additional interest for biosensor layouts: Their repetitive protein subunits arranged at nanometric spacing can be employed for coupling functional molecules. If used as adapters on sensor chip surfaces, they allow an efficient immobilization of analyte-specific recognition and detector elements such as antibodies and enzymes at highest surface densities. The display on plant viral bionanoparticles may also lead to long-time stabilization of sensor molecules upon repeated uses and has the potential to increase sensor performance substantially, compared to conventional layouts. This has been demonstrated in different proof-of-concept biosensor devices. Therefore, richly available plant viral particles, non-pathogenic for animals or humans, might gain novel importance if applied in receptor layers of FEDs. These perspectives are explained and discussed with regard to future detection strategies for COVID-19 and related viral diseases.

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Green Nanoparticles for Biomedical and Bioengineering Applications

Cited by 7 publications

References 165 publications

Scaling-up strategies for controllable biosynthetic ZnO NPs using cell free-extract of endophytic Streptomyces albus: characterization, statistical optimization, and biomedical activities evaluation

Scaling-up strategies for controllable biosynthetic ZnO NPs using cell free-extract of endophytic Streptomyces albus: characterization, statistical optimization, and biomedical activities evaluation

Capítulo 4 - Síntese verde de nanomateriais

Field-Effect Sensors for Virus Detection: From Ebola to SARS-CoV-2 and Plant Viral Enhancers

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