Green fabricated CuO nanobullets via Olea europaea leaf extract shows auspicious antimicrobial potential

Abstract

In present investigation, copper oxide (CuO) nanostructures have been prepared via green chemistry. leaf extract act as strong chelating agent for tailoring physical as well as bio-medical characteristics of CuO at the nano-size. Physical characterisation such as scanning electron microscope analysis depicts the formation of homogenised spherical shape nanoparticles (NPs) with average size of 42 nm. X-ray diffraction and Fourier transform infrared spectroscopy further confirmed the crystalline pure phase and monoclinic structure. High performance liquid chromatography (HPLC) testing is performed to evaluate the relative concentration of bioactive molecules in the leaf extract. From HPLC results capping action of organic molecules around CuO-NPs is hypothesised. The antimicrobial potency of biosynthesised CuO-NPs have been evaluated using colony forming unit (CFU) counting assay and disc diffusion method which shows a significant zone of inhibition against bacterial and fungal strains may be highly potential for future antimicrobial pharmaceutics. Furthermore, reduction of various precursors by plant extract will reduce environmental impact over chemical synthesis.

RETRACTED: CuO and CeO2 Nanostructures Green Synthesized Using Olive Leaf Extract Inhibits the Growth of Highly Virulent Multidrug Resistant Bacteria

“…Smaller size and positively charged NS can easily be attracted by negatively charged bacterial surface. This electrostatic force of attraction is quite helpful in auto-phagocytosis of NS inside the bacterial cellular environment ( Maqbool et al, 2016 , 2017 ). Normally, it has been observed that due to the presence of additional mucus layer (lipopolysaccharide), it is a tough task for any drug molecule to penetrate across it.…”

“…Maximum penetration of the bacterial cell wall by NS has been observed due to opposite charges of attraction. These unique properties of multimode of actions and maximum invasion are quite helpful in developing NS as broad-spectrum antibiotics in the future ( Maqbool et al, 2017 ; Ni et al, 2017 ).…”

“…All these characteristics are directly linked to variable oxidation states of Ce (Ce 3+ , Ce 4+ ) and photoexcitaion at room temperature through which the NS can generate lethal reactive oxygen species (ROS) ( Maria Magdalane et al, 2017 ). When tested against lethal strains like Escherichia coli , Staphylococcus aureus , and Pseudomonas aeruginosa , CuO-NS showed moderate antibacterial behavior ( Maqbool et al, 2017 ).…”

Green synthesis of copper oxide nanoparticles for biomedical application and environmental remediation

“…The probable applications of copper oxide nanoparticles (CuONPs) in gas sensors, waste treatment, catalysis, batteries, food preservation, high temperature superconductors, solar energy conversion, photovoltaic devices, dye removal, field emission emitters and in agriculture have been established [ 11 , 12 , 13 , 14 ]. Aside the previously mentioned usage, CuONPs showed exclusive anticancer, antimicrobial and antioxidant efficacy which renders them a promising tool for biomedical applications [ 15 , 16 ]. Different physical and chemical approaches like microwave irradiation, thermal decomposition, sol gel, colloidal thermal synthesis, sonochemical, hydrothermal, and quick precipitation have been reported for the synthesis of CuONPs with desired morphologies qualities [ 17 ].…”

Optimised synthesis of ZnO‐nano‐fertiliser through green chemistry: boosted growth dynamics of economically important L. esculentum

“…NS encloses a wide range of modern applications due to their extremely sized, tuned structure and physio‐chemical properties in agriculture industries [6, 7]. Although there are so many routes to engineer application targeted NS but plants were proved to be the best choice due to least cytotoxicity and greater biocompatibility [8]. Evolution in green chemistry had resulted in the development of traditional agriculture methodologies to modern industrial agriculture in the last decade.…”