Green Emitting Photoproducts from Terrylene Diimide after Red Illumination

Abstract

The spectral properties of emissive photoproducts, formed upon 633 nm irradiation of a terrylene diimide dye, were investigated. Ensemble and single-molecule level experiments were conducted by immobilizing the TDI dye molecules in a polystyrene film. In the bulk experiments, green emission could be observed from the photobleached areas (photobleached with 633 nm light) when excited with 480 or 514 nm light. Similar phenomena were also observed at the single-molecule level. On the basis of the single-molecule experiments, a conversion efficiency of about 5% was estimated for the formation of emissive spectrally blue-shifted photoproducts. These green emissive photoproducts have spectral properties that resemble those of lower rylene homologues, e.g. perylene diimide or perylene monoimide. Our results indicate that the formation of blue-shifted emissive photoproducts can have implications for analyzing single-molecule FRET experiments or multiple color-labeled fluorescent systems.

Single‐Molecule Characterization of Near‐Infrared‐Emitting Silver Nanoclusters

“…Single Molecule Measurements : Single molecule measurements were made on a homebuilt confocal microscope described in detail by Liao et al [ 52 ] Briefl y, a sample of silver nanoclusters was diluted in a saturated solution of PVA (Aldrich) in MQ water. This solution was spincast onto a clean glass coverslip (Menzel 22 mm × 22 mm × 170 µm).…”

Upconversion Cross‐Correlation Spectroscopy of a Sandwich Immunoassay

“…Upconversion Cross‐Correlation Spectroscopy . UCCS time traces were acquired with a home‐build confocal microscope . The setup is shown in Figure S8 in the Supporting Information along with all optical components.…”

“…UCCS time traces were acquired with ah ome-build confocal microscope. [19] The setup is shown in Figure S8 in the Supporting Information along with all optical components. We used the output of ac ontinuous wave Ti-sapphire laser (3900S Spectra-Physics) which was tuned to an emission wavelength of 977 nm.…”

Crystal structure of a NIR‐Emitting DNA‐Stabilized Ag16 Nanocluster

“…When excited at 520 nm, the individual polycrystals’ emission spectra, recorded using confocal microscopy, have very similar FWHM (2751 cm −1 for the crystals and 2528 cm −1 for the solution, including the Jacobian correction), but are slightly blue‐shifted ( ≈720 nm) with respect to the solution spectrum. The fluorescence spectra of five different crystals can be found in Figure S1 in the Supporting Information.…”