Green Coffee Extract Protects H9C2 Cardiomyocytes from Doxorubicin Induced Apoptosis

Abstract

Green Coffee Extract (GCE), an extract of Coffea arabica bean is a popular health supplement employed for anti-obesity and anti-diabetic effects. Here a hydroalcoholic extract of Green Coffee (GCE) was evaluated for its potential as a cardioprotective agent against Doxorubicin (Dox) induced cardiac insult in a H9C2 rat cardiomyocyte in vitro model system. The GCE was tested in an MTT viability assay using 1 μM Dox with and without GCE pretreatment at 50, 100 and 250 μg mLG 1 concentrations. GCE was also tested for its free radical scavenging ability in a DPPH assay at 10 concentrations (500 μg mLG 1 maximum concentration). To understand the mechanism of action of cardioprotection, mitochondrial membrane potential (Δψm) was compared between Dox treated cells with and without GCE pretreatment, using the JC-1 dye. Finally, the activation of caspase-3/7 was quantitated. Findings from the above experiments demonstrated that GCE rescued H9C2 cardiomyocytes from Dox induced loss of cell viability in a dose-dependent manner. While Dox treatment caused a clear decrease in the JC-1 ratio from 2 to 1.6 due to loss of Δψm, pretreatment with GCE at 25, 50 and 100 μg mLG 1 restored the JC-1 ratio to 1.6, 1.9 and 2.0, respectively. Dox treatment potently induced caspase 3/7 activity by 5 fold and pre-treatment with GCE at 100 and 500 μg mLG 1 reduced this activation to 3.5 and 1.5 fold, respectively. This data clearly demonstrates that GCE is strongly cardioprotective against Dox induced cardiac insult and the mechanism of action is by blocking activation of intrinsic apoptotic pathway.

Periostin alters transcriptional profile in a rat model of isoproterenol-induced cardiotoxicity

“…Preventing Casp7 activation is a strong indicator of anti-apoptotic capacity. Malkapuram et al 54 demonstrated that prevention of Casp7 activation protects H9C2 cells from DOX-induced apoptosis. Our findings showed that periostin application downregulated myocardial expression of apoptotic executioner gene Casp7 from caspase family in the ISO-induced myocardial injury model in rats, which may reflect antiapoptotic function of periostin.…”