Nanotechnology is emerging as a rapidly growing field with its wide application in science andtechnology for manufacturing of new materials at nanoscale level .This study aims to examine the effect of the nanoparticles of cellulose from grape seeds on obese hyperlipidemic rats.
Methods:Cellulose of grape seed were isolated by 15% NaoH at 160∘ C for two hours, according to .Cellulose Nanocrystals (CNC) were isolated from grapes bleached pulp by acid hydrolysis using 65% sulphuric acid at 45°C for 45 minutes as previously described . Thirty (30) (urea , uric acid & creatinine) were determined.
male albino rats of Sprague Dawley strain divided into six groups. Group(1) fed on the basal diet (negative control), (2)fed on the hyperlipidemic diet (HD) (positive control), (3) fed on HD + 2% nanoparticles from grape seeds (NGS), (4) fed on HD +4% NGS, (5) fed on HD +5% grape seeds powder (GSP), (6) fed on HD +10% GSP.
Total Phenolic Compounds ,Concentration Phenolic Compounds, (SEM) examination, food intake, body weight gain ratio, lipids profile (Total lipids TL, Triglycerides TG, Total cholesterol TC, LDL, HDL & VLDL ) , liver functions (AST, & ALT) , Total protein , albiumine , kidney functions
Results: The results observed that rats treatment with nanocellulose from grape seeds (NGS 2%& 4%) had significantly decrease in body weight gain ratio (BWG%) and food intake; when compared with the positive control rats. Moreover, rats treatment with nanocellulose from grape seeds (NGS 2%& 4%) had significantly decrease in lipids profile levels (TL, TG, TC, LDL & VLDL), Liver function & Kidney functions than rats fed on HD+GSP when compared with the positive control ratsConclusions: It can be concluded that nanoparticles isolated from red grape seed specially Cellulose Nanocrystals (CNC) had a significant positive effects against obesity and Hyperlipidemic than the effects of Grape Seeds Powder. Hence, the study may be repeated on patients with hyperlipidemia for possible beneficial effect on humans.