“Green” Approach to Propolis Extraction: Natural Deep Eutectic Solvents

Trusheva, Boryana; Petkov, Hristo; Popova, Milena; Dimitrova, Lyudmila; Zaharieva, Maya M.; Tsvetkova, Iva; Najdenski, Hristo; Bankova, Vassya

doi:10.7546/crabs.2019.07.06

Cited by 9 publications

(9 citation statements)

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“…In most cases where antimicrobial activity was observed, it was much higher than the one of the traditional ethanol extracts. Such antimicrobial potentiating effects of NADESs was also observed in the case of propolis NADES extracts [ 14 ]. Although the addition of more than 50% of water to NADES during the antimicrobial tests breaks the NADES supramolecular complex, the individual NADES constituents could contribute to the overall effect of the solution [ 15 ] and some synergistic effects are also to be considered.…”

Section: Resultsmentioning

confidence: 69%

Extracts of medicinal plants with natural deep eutectic solvents: enhanced antimicrobial activity and low genotoxicity

et al. 2020

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Natural deep eutectic solvents (NADES) are a new alternative to toxic organic solvents. Their constituents are primary metabolites, non-toxic, biocompatible and sustainable. In this study four selected NADES were applied for the extraction of two medicinal plants: Sideritis scardica, and Plantago major as an alternative to water-alcohol mixtures, and the antimicrobial and genotoxic potential of the extracts were studied. The extraction efficiency was evaluated by measuring the extracted total phenolics, and total flavonoids. Best extraction results for total phenolics for the studied plants were obtained with choline chloride-glucose 5:2 plus 30% water; but surprisingly these extracts were inactive against all tested microorganisms. Extracts with citric acid-1,2-propanediol 1:4 and choline chloride-glycerol 1:2 showed good activity against S. pyogenes, E. coli, S. aureus, and C. albicans. Low genotoxicity and cytotoxicity were observed for all four NADES and the extracts with antimicrobial activity. Our results confirm the potential of NADESs for extraction of bioactive constituents of medicinal plants and further suggest that NADES can improve the effects of bioactive extracts. Further studies are needed to clarify the influence of the studied NADES on the bioactivity of dissolved substances, and the possibility to use such extracts in the pharmaceutical and food industry.

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Section: Resultsmentioning

confidence: 69%

Extracts of medicinal plants with natural deep eutectic solvents: enhanced antimicrobial activity and low genotoxicity

et al. 2020

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“…Some studies indicate the effectiveness of the use of some ES and IL in the extraction of flavonoids from several natural products, considering conventional solvents. However, no reports compare these solvents in samples of Brazilian red propolis, which have stood out for their biological activities ( Koutsoukos et al, 2019 ; Trusheva et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…Since alcohol is used as the most common extractor among propolis extracts by several authors ( Alencar et al, 2007 ; da Silva Frozza et al, 2013 ; Santana Andrade et al, 2017 ), equivalence in the extractive power of ES is crucial to obtain the exact yield using an alternative solvent. Trusheva et al (2019) tested the extract with choline chloride and glycerol, and it showed 8.4% of total flavones and flavonols compared to 70% ethanol (11.3%) in propolis samples of poplar type. Funari et al (2019) tested the same ES, resulting in a lower performance of phenolic compounds than 70% ethanol in green propolis samples.…”

Section: Resultsmentioning

confidence: 99%

Red Propolis as a Source of Antimicrobial Phytochemicals: Extraction Using High-Performance Alternative Solvents

et al. 2021

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Propolis is a resinous material rich in flavonoids and involved in several biological activities such as antimicrobial, fungicide, and antiparasitic functions. Conventionally, ethanolic solutions are used to obtain propolis phytochemicals, which restrict their use in some cultures. Given this, we developed an alcohol-free high-performance extractive approach to recover antibacterial and antioxidants phytochemicals from red propolis. Thus, aqueous-solutions of ionic liquids (IL) and eutectic solvents were used and then tested for their total flavonoids, antioxidant, and antimicrobial activities. The surface-responsive technique was applied regarding some variables, namely, the time of extraction, the number of extractions, and cavitation power (W), to optimize the process (in terms of higher yields of flavonoids and better antioxidant activity). After that, four extractions with the same biomass (repetitions) using 1-hexyl-3-methylimidazolium chloride [C6mim]Cl, under the operational conditions fixed at 3.3 min and 300 W, were able to recover 394.39 ± 36.30 mg RuE. g−1 of total flavonoids, with total antioxidant capacity evaluated up to 7595.77 ± 5.48 μmol TE. g−1dried biomass, besides inhibiting the growth of Staphylococcus aureus and Salmonella enteritidis bacteria (inhibition halo of 23.0 ± 1.0 and 15.7 ± 2.1, respectively). Aiming at the development of new technologies, the antimicrobial effect also presented by [C6mim]Cl may be appealing, and future studies are required to understand possible synergistic actions with propolis phytochemicals. Thereby, we successfully applied a completely alcohol-free method to obtain antimicrobials phytochemicals and highly antioxidants from red propolis, representing an optimized process to replace the conventional extracts produced until now.

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“…The aqueous extract presented twice the extractive potential of 95 % ethanol, an increase attributed to the presence of the ionic liquid, as its higher hydrophobicity leads to the transfer of hydrophobic components from the biomass based on π–π, n–π, and hydrophobic interactions. The eutectic solvents proposed by Trusheva et al [35] involved citric acid or choline chloride mixed with alcohols. Temperature has seldom exceeded 60°C, to prevent degradation of the bioactive compounds [2] , [35] , [36] .…”

Section: Introductionmentioning

confidence: 99%

“…The eutectic solvents proposed by Trusheva et al [35] involved citric acid or choline chloride mixed with alcohols. Temperature has seldom exceeded 60°C, to prevent degradation of the bioactive compounds [2] , [35] , [36] . Golmahi et al .…”

Section: Introductionmentioning

confidence: 99%

Do ultrasonic field effects upon the polyphenolics profile of propolis extracts improve their antioxidant and antimicrobial activity?

Nichitoi

Josceanu

Isopescu

et al. 2023

Ultrasonics Sonochemistry

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“Green” Approach to Propolis Extraction: Natural Deep Eutectic Solvents

Cited by 9 publications

References 0 publications

Extracts of medicinal plants with natural deep eutectic solvents: enhanced antimicrobial activity and low genotoxicity

Extracts of medicinal plants with natural deep eutectic solvents: enhanced antimicrobial activity and low genotoxicity

Red Propolis as a Source of Antimicrobial Phytochemicals: Extraction Using High-Performance Alternative Solvents

Do ultrasonic field effects upon the polyphenolics profile of propolis extracts improve their antioxidant and antimicrobial activity?

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