Green and brown bridges between weeds and crops reveal novel &lt;I&gt;Diaporthe&lt;/I&gt; species in Australia

Thompson, S. M.; Tan, Yu Pei; Shivas, R. G.; Neate, Stephen M.; Morin, L.; Bissett, Andrew; Aitken, E. A. B.

doi:10.3767/003158515x687506

Cited by 83 publications

(53 citation statements)

References 50 publications

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“…Diaporthecaryae can be distinguished based o n ITS, tef1 and tub2 loci from D.charlesworthii (50/468 in ITS, 107/338 in tef1 and 90/707 in tub2 ); from D.sackstonii (4/440 in ITS, 13/340 in tef1 and 23/701 in tub2 ). Morphologically, D.caryae can be distinguished from D.charlesworthii by its shorter conidiophores (7–11 vs. 15–35 μm); from D.sackstonii by its longer alpha conidia (7–8.5 vs. 6–7 μm) (Thompson et al 2015). …”

Section: Resultsmentioning

confidence: 99%

High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described

2018

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Diaporthe species have often been reported as important plant pathogens, saprobes and endophytes on a wide range of plant hosts. Although several Diaporthe species have been recorded in China, little is known about species able to infect forest trees. Therefore, extensive surveys were recently conducted in Beijing, Heilongjiang, Jiangsu, Jiangxi, Shaanxi and Zhejiang Provinces. The current results emphasised on 15 species from 42 representative isolates involving 16 host genera using comparisons of DNA sequence data for the nuclear ribosomal internal transcribed spacer (ITS), calmodulin (cal), histone H3 (his3), partial translation elongation factor-1α (tef1) and β-tubulin (tub2) gene regions, as well as their morphological features. Three known species, D.biguttulata, D.eres and D.unshiuensis, were identified. In addition, twelve novel taxa were collected and are described as D.acerigena, D.alangii, D.betulina, D.caryae, D.cercidis, D.chensiensis, D.cinnamomi, D.conica, D.fraxinicola, D.kadsurae, D.padina and D.ukurunduensis. The current study improves the understanding of species causing diebacks on ecological and economic forest trees and provides useful information for the effective disease management of these hosts in China.

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Section: Resultsmentioning

confidence: 99%

High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described

2018

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“…Diaporthe spp. are pathogens of many different plant species and cause seed rot, stem cankers, lesions, and pod blight [68], but in common beans they are simply endophytes [69]. Therefore, it is remarkable that the two isolates of Diaporthe from seedlot of ‘INTA Rojo’ caused severe disease symptoms in common bean seedlings (Fig 4).…”

Section: Discussionmentioning

confidence: 99%

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

Marcenaro

Valkonen

2016

PLoS ONE

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Common bean (Phaseolus vulgaris L.) is an important legume with high nutritional value. In Nicaragua, certified healthy seeds of local bean varieties are not available, and seedborne fungi have gained little attention. Here, were surveyed seedborne pathogenic fungi in an important local bean cultivar, ‘INTA Rojo’. Beans grown in the four main production areas in Nicaragua (Boaco, Carazo, Estelí, Matagalpa) for future use as seed stock were sampled from four seed storehouses and six seed lots. A total of 133 fungal strains were isolated from surface-sterilized beans and inoculated to healthy lima beans (Phaseolus lunatus) under controlled conditions. Eighty-seven isolates caused symptoms of varying severity in the seedlings, including discoloration, necrotic lesions, cankers, rot, and lethal necrosis. Pathogenic isolates were divided into eight phenotypically distinguishable groups based on morphology and growth characteristics on artificial growth medium, and further identified by analysis of the internal transcribed spacer sequences (ITS1 and ITS2) of the ribosomal RNA genes. The pathogenic isolates belonged to eight genera. Fusarium spp. (F. chlamydosporum, F. equiseti, F. incarnatum), Lasiodiplodia theobromae, Macrophomina phaseolina, and Penicillium citrinum were the most damaging and common fungi found in the seed lots. Furthermore, Corynespora cassiicola, Colletotrichum capsisi, Colletotrichum gloeosporioides, Aspergillus flavus, and Diaporthe sp. (Phomopsis) were seedborne in cultivar ‘INTA Rojo’ and found to be pathogenic to bean seedlings. This study reveals, for the first time, many seedborne pathogenic fungi in beans in Nicaragua; furthermore, prior to this study, little information was available concerning F. equiseti, F. incarnatum, L. theobromae, C. cassiicola, and Diaporthe spp. as seedborne pathogens of common bean. Our results lay the basis for developing diagnostic tools for seed health inspection and for further study of the epidemiology, ecology, and control of the pathogenic fungi of common beans in the field.

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“…Phomopsis) have been primarily based on morphology, which has been shown to pay a minor role in species delimitation due to the simple and plastic morphological characters (Huang et al 2013, Gao et al 2016, Du et al 2016, Yang et al 2017a. Thus, analyses of rDNA ITS coupled with morphology, pathogenicity or multi-locus sequences data have been used in successful taxonomic revisions in contemporary molecular phylogenetic studies (Farr et al 2002a, Santos & Phillips 2009, Diogo et al 2010, Santos et al 2011, Thompson et al 2015, Udayanga et al 2011, 2012a, 2012b, 2014a, 2014c, Gomes et al 2013, Huang et al 2015, Gao et al 2015, Fan et al 2015, Du et al 2016. But confusion occurs when large number of species from a wide range of host species are analyzed.…”

Section: Discussionmentioning

confidence: 99%

Phylogeny and morphology reveal two new species of Diaporthe from Traditional Chinese Medicine in Northeast China

Yang

Tian

2018

Phytotaxa

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Diaporthe sambucusii sp. nov and D. schisandrae sp. nov., collected from diseased branches of Traditional Chinese Medicine in Northeast China, Sambucus williamsii Hance and Schisandra chinensis (Turcz.) Baill, are herein described and illustrated. Recognition of the two new species are supported by both holomorphic morphology and phylogenetic analysis. Morphologically, D. sambucusii is distinguished by hyaline, aseptate, smooth, ovoid to subfusiform, biguttulate alpha conidia (7.0-9.5 × 2.0−2.5 μm) and beta conidia hyaline, aseptate, straight to hamate, 18.5-23.5 × 0.9-1.1 μm, similar to most species of Diaporthe. Diaporthe schisandrae is characterized by hyaline, aseptate, ellipsoidal to fusoid, somewhat tubercular at one end and obtuse at other, 1-3-guttulate alpha conidia (8.5-11.5 × 2.7−3.3 μm). Phylogenetic analysis using CAL, HIS, ITS, TEF1-α, and TUB molecular data shows that the isolates of the new species form two distinct clade within Diaporthe (MP/ML/BI=100/100/1).

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Green and brown bridges between weeds and crops reveal novel <I>Diaporthe</I> species in Australia

Cited by 83 publications

References 50 publications

High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described

High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

Phylogeny and morphology reveal two new species of Diaporthe from Traditional Chinese Medicine in Northeast China

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